SIMPLIFIED LIQUID-LIQUID EXTRACTION METHOD COUPLED WITH LC/DAD BY QUANTITATIVE DETERMINATION OF RISPERIDONE IN HUMAN SERUM

ABSTRACT Risperidone, an antipsychotic drug, was determined in human serum, using an optimized liquid-liquid extraction for isolation from biological fluid, with ethyl acetate as extraction solvent. This sample extraction method was chosen to increase extraction efficiency and reduce matrix interfer...

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Autores principales:	Mennickent,Sigrid, Diego,Marta De, Liser,Bernd, Trujillo,Lina
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2019
Materias:	Risperidone human serum liquid chromatography quantitative determination
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072019000104265
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spelling	oai:scielo:S0717-970720190001042652019-04-11SIMPLIFIED LIQUID-LIQUID EXTRACTION METHOD COUPLED WITH LC/DAD BY QUANTITATIVE DETERMINATION OF RISPERIDONE IN HUMAN SERUMMennickent,SigridDiego,Marta DeLiser,BerndTrujillo,Lina Risperidone human serum liquid chromatography quantitative determination ABSTRACT Risperidone, an antipsychotic drug, was determined in human serum, using an optimized liquid-liquid extraction for isolation from biological fluid, with ethyl acetate as extraction solvent. This sample extraction method was chosen to increase extraction efficiency and reduce matrix interferences. Quantitation was achieved by LC/DAD at 294 nm over the concentration range of 1 ng/mL to 60 ng/mL (r=0.999). Desipramine was used as internal standard (tR for Risperidone: 4.23; tR Desipramine: 7.31). Mobile phase was water: acetonitrile: triethilamine: glacial acetic acid (70:29.16:0.52: 0.33 v/v), using a Purosphere STAR RP-18e 250 × 4.5 mm (5µ) column. RSD, for the intra-assay study, was between 0.52 % – 2.09 % (n=3), and between 0.79 % −3.25 % (n=9) for the inter-assay. LOD was 0.40 ng/mL and LOQ was 0.87 ng/mL. Recovery percentage for the accuracy study was between 90.82% and 98.56% ((RSD ≤ 3.93). The method is simple, fast, precise, accurate, sensible and selective. This method was successfully applied to quantify risperidone in patient serum samples. In conclusion, the method is useful for quantitative determination of risperidone in human serum.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.64 n.1 20192019-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072019000104265en10.4067/s0717-97072019000104265
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Risperidone human serum liquid chromatography quantitative determination
spellingShingle	Risperidone human serum liquid chromatography quantitative determination Mennickent,Sigrid Diego,Marta De Liser,Bernd Trujillo,Lina SIMPLIFIED LIQUID-LIQUID EXTRACTION METHOD COUPLED WITH LC/DAD BY QUANTITATIVE DETERMINATION OF RISPERIDONE IN HUMAN SERUM
description	ABSTRACT Risperidone, an antipsychotic drug, was determined in human serum, using an optimized liquid-liquid extraction for isolation from biological fluid, with ethyl acetate as extraction solvent. This sample extraction method was chosen to increase extraction efficiency and reduce matrix interferences. Quantitation was achieved by LC/DAD at 294 nm over the concentration range of 1 ng/mL to 60 ng/mL (r=0.999). Desipramine was used as internal standard (tR for Risperidone: 4.23; tR Desipramine: 7.31). Mobile phase was water: acetonitrile: triethilamine: glacial acetic acid (70:29.16:0.52: 0.33 v/v), using a Purosphere STAR RP-18e 250 × 4.5 mm (5µ) column. RSD, for the intra-assay study, was between 0.52 % – 2.09 % (n=3), and between 0.79 % −3.25 % (n=9) for the inter-assay. LOD was 0.40 ng/mL and LOQ was 0.87 ng/mL. Recovery percentage for the accuracy study was between 90.82% and 98.56% ((RSD ≤ 3.93). The method is simple, fast, precise, accurate, sensible and selective. This method was successfully applied to quantify risperidone in patient serum samples. In conclusion, the method is useful for quantitative determination of risperidone in human serum.
author	Mennickent,Sigrid Diego,Marta De Liser,Bernd Trujillo,Lina
author_facet	Mennickent,Sigrid Diego,Marta De Liser,Bernd Trujillo,Lina
author_sort	Mennickent,Sigrid
title	SIMPLIFIED LIQUID-LIQUID EXTRACTION METHOD COUPLED WITH LC/DAD BY QUANTITATIVE DETERMINATION OF RISPERIDONE IN HUMAN SERUM
title_short	SIMPLIFIED LIQUID-LIQUID EXTRACTION METHOD COUPLED WITH LC/DAD BY QUANTITATIVE DETERMINATION OF RISPERIDONE IN HUMAN SERUM
title_full	SIMPLIFIED LIQUID-LIQUID EXTRACTION METHOD COUPLED WITH LC/DAD BY QUANTITATIVE DETERMINATION OF RISPERIDONE IN HUMAN SERUM
title_fullStr	SIMPLIFIED LIQUID-LIQUID EXTRACTION METHOD COUPLED WITH LC/DAD BY QUANTITATIVE DETERMINATION OF RISPERIDONE IN HUMAN SERUM
title_full_unstemmed	SIMPLIFIED LIQUID-LIQUID EXTRACTION METHOD COUPLED WITH LC/DAD BY QUANTITATIVE DETERMINATION OF RISPERIDONE IN HUMAN SERUM
title_sort	simplified liquid-liquid extraction method coupled with lc/dad by quantitative determination of risperidone in human serum
publisher	Sociedad Chilena de Química
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072019000104265
work_keys_str_mv	AT mennickentsigrid simplifiedliquidliquidextractionmethodcoupledwithlcdadbyquantitativedeterminationofrisperidoneinhumanserum AT diegomartade simplifiedliquidliquidextractionmethodcoupledwithlcdadbyquantitativedeterminationofrisperidoneinhumanserum AT liserbernd simplifiedliquidliquidextractionmethodcoupledwithlcdadbyquantitativedeterminationofrisperidoneinhumanserum AT trujillolina simplifiedliquidliquidextractionmethodcoupledwithlcdadbyquantitativedeterminationofrisperidoneinhumanserum
_version_	1714200933268717568

SIMPLIFIED LIQUID-LIQUID EXTRACTION METHOD COUPLED WITH LC/DAD BY QUANTITATIVE DETERMINATION OF RISPERIDONE IN HUMAN SERUM

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