IMMOBILIZATION OF LEVANSUCRASE: STRATEGIES AND BIOTECHNOLOGICAL APPLICATIONS

ABSTRACT Immobilization is an excellent tool for enzymatic stabilization, improving the biocatalytic processes, allowing the reuse of the enzyme and promoting an easier separation of the molecule of interest. Currently, new enzymatic bonding processes are arising on solid supports, based on classica...

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Autores principales:	Bersaneti,Gabrielly Terassi, Baldo,Cristiani, Colabone Celligoi,Maria Antonia Pedrine
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2019
Materias:	Applications enzymatic immobilization levansucrase supports
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072019000104377
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spelling	oai:scielo:S0717-970720190001043772019-04-11IMMOBILIZATION OF LEVANSUCRASE: STRATEGIES AND BIOTECHNOLOGICAL APPLICATIONSBersaneti,Gabrielly TerassiBaldo,CristianiColabone Celligoi,Maria Antonia Pedrine Applications enzymatic immobilization levansucrase supports ABSTRACT Immobilization is an excellent tool for enzymatic stabilization, improving the biocatalytic processes, allowing the reuse of the enzyme and promoting an easier separation of the molecule of interest. Currently, new enzymatic bonding processes are arising on solid supports, based on classical immobilization methods. Amongst the supports used, chitosan is a polysaccharide that offers a unique set of characteristics, as biocompatibility, biodegradability, non-toxicity and antibacterial properties. Thus, many enzymes has being immobilized on this support, including levansucrase, that is able to synthesize levan and fructooligosaccharides, two important biomolecules which have beneficial health properties. These review present different methods of immobilization (physical adsorption, entrapment, crosslinking and covalent bonding) for fructosyltransferases, as well as different immobilization matrices that can be applied in biotechnological processes. However, studies are still needed in order to adopt efficient immobilization techniques, in which the biocatalyst remains more stable, in order to become the process attractive to the industrial sector.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.64 n.1 20192019-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072019000104377en10.4067/s0717-97072019000104377
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Applications enzymatic immobilization levansucrase supports
spellingShingle	Applications enzymatic immobilization levansucrase supports Bersaneti,Gabrielly Terassi Baldo,Cristiani Colabone Celligoi,Maria Antonia Pedrine IMMOBILIZATION OF LEVANSUCRASE: STRATEGIES AND BIOTECHNOLOGICAL APPLICATIONS
description	ABSTRACT Immobilization is an excellent tool for enzymatic stabilization, improving the biocatalytic processes, allowing the reuse of the enzyme and promoting an easier separation of the molecule of interest. Currently, new enzymatic bonding processes are arising on solid supports, based on classical immobilization methods. Amongst the supports used, chitosan is a polysaccharide that offers a unique set of characteristics, as biocompatibility, biodegradability, non-toxicity and antibacterial properties. Thus, many enzymes has being immobilized on this support, including levansucrase, that is able to synthesize levan and fructooligosaccharides, two important biomolecules which have beneficial health properties. These review present different methods of immobilization (physical adsorption, entrapment, crosslinking and covalent bonding) for fructosyltransferases, as well as different immobilization matrices that can be applied in biotechnological processes. However, studies are still needed in order to adopt efficient immobilization techniques, in which the biocatalyst remains more stable, in order to become the process attractive to the industrial sector.
author	Bersaneti,Gabrielly Terassi Baldo,Cristiani Colabone Celligoi,Maria Antonia Pedrine
author_facet	Bersaneti,Gabrielly Terassi Baldo,Cristiani Colabone Celligoi,Maria Antonia Pedrine
author_sort	Bersaneti,Gabrielly Terassi
title	IMMOBILIZATION OF LEVANSUCRASE: STRATEGIES AND BIOTECHNOLOGICAL APPLICATIONS
title_short	IMMOBILIZATION OF LEVANSUCRASE: STRATEGIES AND BIOTECHNOLOGICAL APPLICATIONS
title_full	IMMOBILIZATION OF LEVANSUCRASE: STRATEGIES AND BIOTECHNOLOGICAL APPLICATIONS
title_fullStr	IMMOBILIZATION OF LEVANSUCRASE: STRATEGIES AND BIOTECHNOLOGICAL APPLICATIONS
title_full_unstemmed	IMMOBILIZATION OF LEVANSUCRASE: STRATEGIES AND BIOTECHNOLOGICAL APPLICATIONS
title_sort	immobilization of levansucrase: strategies and biotechnological applications
publisher	Sociedad Chilena de Química
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072019000104377
work_keys_str_mv	AT bersanetigabriellyterassi immobilizationoflevansucrasestrategiesandbiotechnologicalapplications AT baldocristiani immobilizationoflevansucrasestrategiesandbiotechnologicalapplications AT colabonecelligoimariaantoniapedrine immobilizationoflevansucrasestrategiesandbiotechnologicalapplications
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IMMOBILIZATION OF LEVANSUCRASE: STRATEGIES AND BIOTECHNOLOGICAL APPLICATIONS

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