A PROMISING DNA GROOVE BINDER AND PHOTOCLEAVER BASED ON A DINUCLEAR RUTHENIUM(II) COMPLEX

ABSTRACT A dinuclear ruthenium complex [Ru2(bpy)4(bip-phenol)](ClO4)4 {bpy = 2,2′-bipyridine, bip-phenol = 2,4-bis(1H-imidazo[4,5-f][1,10]phenanthrolin-2-yl)phenol} has been synthesized and characterized. The calf thymus (ct) DNA binding properties of the complex are investigated by means...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mi,Ya-Xuan, Wang,Shuang, Xu,Xue-Xue, Zhao,Hua-Qian, Zheng,Ze-Bao, Zhao,Xiao-Long
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2019
Materias:	Ruthenium(II) complex Calf thymus DNA Groove mode
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072019000104392
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-97072019000104392
record_format	dspace
spelling	oai:scielo:S0717-970720190001043922019-04-11A PROMISING DNA GROOVE BINDER AND PHOTOCLEAVER BASED ON A DINUCLEAR RUTHENIUM(II) COMPLEXMi,Ya-XuanWang,ShuangXu,Xue-XueZhao,Hua-QianZheng,Ze-BaoZhao,Xiao-Long Ruthenium(II) complex Calf thymus DNA Groove mode ABSTRACT A dinuclear ruthenium complex [Ru2(bpy)4(bip-phenol)](ClO4)4 {bpy = 2,2′-bipyridine, bip-phenol = 2,4-bis(1H-imidazo[4,5-f][1,10]phenanthrolin-2-yl)phenol} has been synthesized and characterized. The calf thymus (ct) DNA binding properties of the complex are investigated by means of DNA viscosity and optical spectroscopic techniques of UV-visible absorption and emission spectral titrations, steady-state emission quenching with ferrocyanide, ethidium bromide competitive binding, DNA thermal denaturation and reverse salt effect, together with molecular simulation technology. The results suggest that the complex is a promising DNA groove binder with a large DNA binding constant on 106 M−1 order of magnitude. The fluorescence of the complex manifests by 6.3-fold upon binding saturately to DNA. The complex is also demonstrated to be an efficient photocleaver of pBR 322 DNA.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.64 n.1 20192019-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072019000104392en10.4067/s0717-97072019000104392
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Ruthenium(II) complex Calf thymus DNA Groove mode
spellingShingle	Ruthenium(II) complex Calf thymus DNA Groove mode Mi,Ya-Xuan Wang,Shuang Xu,Xue-Xue Zhao,Hua-Qian Zheng,Ze-Bao Zhao,Xiao-Long A PROMISING DNA GROOVE BINDER AND PHOTOCLEAVER BASED ON A DINUCLEAR RUTHENIUM(II) COMPLEX
description	ABSTRACT A dinuclear ruthenium complex [Ru2(bpy)4(bip-phenol)](ClO4)4 {bpy = 2,2′-bipyridine, bip-phenol = 2,4-bis(1H-imidazo[4,5-f][1,10]phenanthrolin-2-yl)phenol} has been synthesized and characterized. The calf thymus (ct) DNA binding properties of the complex are investigated by means of DNA viscosity and optical spectroscopic techniques of UV-visible absorption and emission spectral titrations, steady-state emission quenching with ferrocyanide, ethidium bromide competitive binding, DNA thermal denaturation and reverse salt effect, together with molecular simulation technology. The results suggest that the complex is a promising DNA groove binder with a large DNA binding constant on 106 M−1 order of magnitude. The fluorescence of the complex manifests by 6.3-fold upon binding saturately to DNA. The complex is also demonstrated to be an efficient photocleaver of pBR 322 DNA.
author	Mi,Ya-Xuan Wang,Shuang Xu,Xue-Xue Zhao,Hua-Qian Zheng,Ze-Bao Zhao,Xiao-Long
author_facet	Mi,Ya-Xuan Wang,Shuang Xu,Xue-Xue Zhao,Hua-Qian Zheng,Ze-Bao Zhao,Xiao-Long
author_sort	Mi,Ya-Xuan
title	A PROMISING DNA GROOVE BINDER AND PHOTOCLEAVER BASED ON A DINUCLEAR RUTHENIUM(II) COMPLEX
title_short	A PROMISING DNA GROOVE BINDER AND PHOTOCLEAVER BASED ON A DINUCLEAR RUTHENIUM(II) COMPLEX
title_full	A PROMISING DNA GROOVE BINDER AND PHOTOCLEAVER BASED ON A DINUCLEAR RUTHENIUM(II) COMPLEX
title_fullStr	A PROMISING DNA GROOVE BINDER AND PHOTOCLEAVER BASED ON A DINUCLEAR RUTHENIUM(II) COMPLEX
title_full_unstemmed	A PROMISING DNA GROOVE BINDER AND PHOTOCLEAVER BASED ON A DINUCLEAR RUTHENIUM(II) COMPLEX
title_sort	promising dna groove binder and photocleaver based on a dinuclear ruthenium(ii) complex
publisher	Sociedad Chilena de Química
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072019000104392
work_keys_str_mv	AT miyaxuan apromisingdnagroovebinderandphotocleaverbasedonadinuclearrutheniumiicomplex AT wangshuang apromisingdnagroovebinderandphotocleaverbasedonadinuclearrutheniumiicomplex AT xuxuexue apromisingdnagroovebinderandphotocleaverbasedonadinuclearrutheniumiicomplex AT zhaohuaqian apromisingdnagroovebinderandphotocleaverbasedonadinuclearrutheniumiicomplex AT zhengzebao apromisingdnagroovebinderandphotocleaverbasedonadinuclearrutheniumiicomplex AT zhaoxiaolong apromisingdnagroovebinderandphotocleaverbasedonadinuclearrutheniumiicomplex AT miyaxuan promisingdnagroovebinderandphotocleaverbasedonadinuclearrutheniumiicomplex AT wangshuang promisingdnagroovebinderandphotocleaverbasedonadinuclearrutheniumiicomplex AT xuxuexue promisingdnagroovebinderandphotocleaverbasedonadinuclearrutheniumiicomplex AT zhaohuaqian promisingdnagroovebinderandphotocleaverbasedonadinuclearrutheniumiicomplex AT zhengzebao promisingdnagroovebinderandphotocleaverbasedonadinuclearrutheniumiicomplex AT zhaoxiaolong promisingdnagroovebinderandphotocleaverbasedonadinuclearrutheniumiicomplex
_version_	1714200936182710272

A PROMISING DNA GROOVE BINDER AND PHOTOCLEAVER BASED ON A DINUCLEAR RUTHENIUM(II) COMPLEX

Ejemplares similares