A SINGLE COLUMN CHIROPTICAL HPLC BATCH ANALYSIS OF TEN AROMATIC ALCOHOLS

A SINGLE COLUMN CHIROPTICAL HPLC BATCH ANALYSIS OF TEN AROMATIC ALCOHOLS

ABSTRACT The HPLC chiral resolution of ten chiral aromatic alcohols were screened using six different polysaccharides-based chiral stationary phases (CSP) and under four normal phase conditions, using UV absorbance as detection. The screening showed that all compounds can be baseline resolved in at...

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Autores principales: Muñoz,Marcelo A., Orellana,María-Paz, Poblete,Daniela
Lenguaje:English
Publicado: Sociedad Chilena de Química 2019
Materias:
HPLC-OR
HPLC-CD
aromatic alcohols
optical rotation detection
circular dichroism detection
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072019000204488
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spelling oai:scielo:S0717-970720190002044882019-10-11A SINGLE COLUMN CHIROPTICAL HPLC BATCH ANALYSIS OF TEN AROMATIC ALCOHOLSMuñoz,Marcelo A.Orellana,María-PazPoblete,Daniela HPLC-OR HPLC-CD aromatic alcohols optical rotation detection circular dichroism detection ABSTRACT The HPLC chiral resolution of ten chiral aromatic alcohols were screened using six different polysaccharides-based chiral stationary phases (CSP) and under four normal phase conditions, using UV absorbance as detection. The screening showed that all compounds can be baseline resolved in at least one of the tested conditions, being the cellulose-methylbenzoate CSP the one with the best performance by resolving the full set. Furthermore, the use of two chiroptical detectors, based in optical rotation and electronic circular dichroism, permitted the ascertain of the elution order for each of the baseline separations. Finally, several options for a single-column batch analysis, in which samples of all compounds are tested for enantiomeric excess and absolute configuration, are proposed to analyze samples from the asymmetric reduction of the corresponding ketones. These options vary in the number of injections, analysis time and reliability, and their choice will mostly depend on the hardware and standards availability.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.64 n.2 20192019-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072019000204488en10.4067/S0717-97072019000204488
institution Scielo Chile
collection Scielo Chile
language English
topic HPLC-OR
HPLC-CD
aromatic alcohols
optical rotation detection
circular dichroism detection
spellingShingle HPLC-OR
HPLC-CD
aromatic alcohols
optical rotation detection
circular dichroism detection
Muñoz,Marcelo A.
Orellana,María-Paz
Poblete,Daniela
A SINGLE COLUMN CHIROPTICAL HPLC BATCH ANALYSIS OF TEN AROMATIC ALCOHOLS
description ABSTRACT The HPLC chiral resolution of ten chiral aromatic alcohols were screened using six different polysaccharides-based chiral stationary phases (CSP) and under four normal phase conditions, using UV absorbance as detection. The screening showed that all compounds can be baseline resolved in at least one of the tested conditions, being the cellulose-methylbenzoate CSP the one with the best performance by resolving the full set. Furthermore, the use of two chiroptical detectors, based in optical rotation and electronic circular dichroism, permitted the ascertain of the elution order for each of the baseline separations. Finally, several options for a single-column batch analysis, in which samples of all compounds are tested for enantiomeric excess and absolute configuration, are proposed to analyze samples from the asymmetric reduction of the corresponding ketones. These options vary in the number of injections, analysis time and reliability, and their choice will mostly depend on the hardware and standards availability.
author Muñoz,Marcelo A.
Orellana,María-Paz
Poblete,Daniela
author_facet Muñoz,Marcelo A.
Orellana,María-Paz
Poblete,Daniela
author_sort Muñoz,Marcelo A.
title A SINGLE COLUMN CHIROPTICAL HPLC BATCH ANALYSIS OF TEN AROMATIC ALCOHOLS
title_short A SINGLE COLUMN CHIROPTICAL HPLC BATCH ANALYSIS OF TEN AROMATIC ALCOHOLS
title_full A SINGLE COLUMN CHIROPTICAL HPLC BATCH ANALYSIS OF TEN AROMATIC ALCOHOLS
title_fullStr A SINGLE COLUMN CHIROPTICAL HPLC BATCH ANALYSIS OF TEN AROMATIC ALCOHOLS
title_full_unstemmed A SINGLE COLUMN CHIROPTICAL HPLC BATCH ANALYSIS OF TEN AROMATIC ALCOHOLS
title_sort single column chiroptical hplc batch analysis of ten aromatic alcohols
publisher Sociedad Chilena de Química
publishDate 2019
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072019000204488
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AT pobletedaniela asinglecolumnchiropticalhplcbatchanalysisoftenaromaticalcohols
AT munozmarceloa singlecolumnchiropticalhplcbatchanalysisoftenaromaticalcohols
AT orellanamariapaz singlecolumnchiropticalhplcbatchanalysisoftenaromaticalcohols
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