ANALYTICAL APPROACHES IN UNVEILING THE COMPATIBILITY OF COLLAGENASE WITH ADDITIVES IN VIEW OF ITS COLLOIDAL DELIVERY

ANALYTICAL APPROACHES IN UNVEILING THE COMPATIBILITY OF COLLAGENASE WITH ADDITIVES IN VIEW OF ITS COLLOIDAL DELIVERY

ABSTRACT The core objective of present work was to investigate the chemical interactions of selected additives with therapeutic enzyme Collagenase (COLG) and determination of its compatibility for futuristic colloidal formulation. Initially, solubility of COLG with additives was determined and emuls...

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Autores principales: Vijay,Landge Anil, Kannan,Krishnamoorthy
Lenguaje:English
Publicado: Sociedad Chilena de Química 2019
Materias:
Collagenase
FTIR-ATR
SDS-PAGE
Circular Dichroism
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072019000304558
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spelling oai:scielo:S0717-970720190003045582019-11-06ANALYTICAL APPROACHES IN UNVEILING THE COMPATIBILITY OF COLLAGENASE WITH ADDITIVES IN VIEW OF ITS COLLOIDAL DELIVERYVijay,Landge AnilKannan,Krishnamoorthy Collagenase FTIR-ATR SDS-PAGE Circular Dichroism ABSTRACT The core objective of present work was to investigate the chemical interactions of selected additives with therapeutic enzyme Collagenase (COLG) and determination of its compatibility for futuristic colloidal formulation. Initially, solubility of COLG with additives was determined and emulsification efficiency of surfactants was examined. FTIR spectra of COLG with its binary blends were compared. Non-denaturing SDS-PAGE and Circular Dichroism (CD) used to investigate primary and secondary structure of enzyme in binary mixture. Finally, the control and accelerated studies was performed by spectroscopy.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.64 n.3 20192019-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072019000304558en10.4067/S0717-97072019000304558
institution Scielo Chile
collection Scielo Chile
language English
topic Collagenase
FTIR-ATR
SDS-PAGE
Circular Dichroism
spellingShingle Collagenase
FTIR-ATR
SDS-PAGE
Circular Dichroism
Vijay,Landge Anil
Kannan,Krishnamoorthy
ANALYTICAL APPROACHES IN UNVEILING THE COMPATIBILITY OF COLLAGENASE WITH ADDITIVES IN VIEW OF ITS COLLOIDAL DELIVERY
description ABSTRACT The core objective of present work was to investigate the chemical interactions of selected additives with therapeutic enzyme Collagenase (COLG) and determination of its compatibility for futuristic colloidal formulation. Initially, solubility of COLG with additives was determined and emulsification efficiency of surfactants was examined. FTIR spectra of COLG with its binary blends were compared. Non-denaturing SDS-PAGE and Circular Dichroism (CD) used to investigate primary and secondary structure of enzyme in binary mixture. Finally, the control and accelerated studies was performed by spectroscopy.
author Vijay,Landge Anil
Kannan,Krishnamoorthy
author_facet Vijay,Landge Anil
Kannan,Krishnamoorthy
author_sort Vijay,Landge Anil
title ANALYTICAL APPROACHES IN UNVEILING THE COMPATIBILITY OF COLLAGENASE WITH ADDITIVES IN VIEW OF ITS COLLOIDAL DELIVERY
title_short ANALYTICAL APPROACHES IN UNVEILING THE COMPATIBILITY OF COLLAGENASE WITH ADDITIVES IN VIEW OF ITS COLLOIDAL DELIVERY
title_full ANALYTICAL APPROACHES IN UNVEILING THE COMPATIBILITY OF COLLAGENASE WITH ADDITIVES IN VIEW OF ITS COLLOIDAL DELIVERY
title_fullStr ANALYTICAL APPROACHES IN UNVEILING THE COMPATIBILITY OF COLLAGENASE WITH ADDITIVES IN VIEW OF ITS COLLOIDAL DELIVERY
title_full_unstemmed ANALYTICAL APPROACHES IN UNVEILING THE COMPATIBILITY OF COLLAGENASE WITH ADDITIVES IN VIEW OF ITS COLLOIDAL DELIVERY
title_sort analytical approaches in unveiling the compatibility of collagenase with additives in view of its colloidal delivery
publisher Sociedad Chilena de Química
publishDate 2019
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072019000304558
work_keys_str_mv AT vijaylandgeanil analyticalapproachesinunveilingthecompatibilityofcollagenasewithadditivesinviewofitscolloidaldelivery
AT kannankrishnamoorthy analyticalapproachesinunveilingthecompatibilityofcollagenasewithadditivesinviewofitscolloidaldelivery
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