IDENTIFICATION OF WATER-SOLUBLE COMPOUNDS CONTAINED IN AQUEOUS EXTRACTS AND FRACTIONS OBTAINED FROM LEAVES OF Ugni Molinae TO DETERMINE THEIR EFFECT ON THE VIABILITY OF HUMAN GASTRIC CANCER CELLS

ABSTRACT Ugni molinae Turcz. Myrtaceae (Murtilla) is a plant native to Chile. There is evidence about the antiproliferative compounds present in fruits, but there is no evidence on the antiproliferative activity of the compounds in the leaves. These compounds have importance because they predominate...

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Autores principales:	Avello,M, Pastene,E, Eillen,Torres
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2020
Materias:	Aqueous extract viability AGS Ugni molinae
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072020000204849
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spelling	oai:scielo:S0717-970720200002048492020-08-14IDENTIFICATION OF WATER-SOLUBLE COMPOUNDS CONTAINED IN AQUEOUS EXTRACTS AND FRACTIONS OBTAINED FROM LEAVES OF Ugni Molinae TO DETERMINE THEIR EFFECT ON THE VIABILITY OF HUMAN GASTRIC CANCER CELLSAvello,MPastene,EEillen,Torres Aqueous extract viability AGS Ugni molinae ABSTRACT Ugni molinae Turcz. Myrtaceae (Murtilla) is a plant native to Chile. There is evidence about the antiproliferative compounds present in fruits, but there is no evidence on the antiproliferative activity of the compounds in the leaves. These compounds have importance because they predominate in the popular form of ingestion of leaves. In the present study, the identification of water-soluble components of leaves of U. molinae and a fractionation were carried out to determine the effect on viability of gastric adenocarcinoma cells (AGS). A decoct of leaves was performed at 1%. For the separation of fractions, a countercurrent system (CPC) was used. Leaves were collected and identified by thinlayer chromatography and liquid chromatography-mass spectrometry (HPLC MS/MS). Samples were evaluated to determine the viability of AGS cells by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidefor (MTT) assays. Three large fractions were identified. In the first one, the phenolic compounds of medium polarity and low molecular weight (phenolic acids) are found. In The second fraction shows low concentrations of phenolic compounds with greater molecular weight (glycosylated and gallolylated flavonoids); and the third, has a predominance of hydrolysable tannins, procianidins and other highly hydrophilic compounds. About cell viability, it was found that, the 1% extract is the one that presented the best effects on cell viability inhibition, in comparation to tannin and flavonoid fractions. In conclusion, there is synergy in the compounds present in the aqueous extract, which causes greater inhibition of cell viability, unlike the separated fractions.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.65 n.2 20202020-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072020000204849en10.4067/S0717-97072020000204849
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Aqueous extract viability AGS Ugni molinae
spellingShingle	Aqueous extract viability AGS Ugni molinae Avello,M Pastene,E Eillen,Torres IDENTIFICATION OF WATER-SOLUBLE COMPOUNDS CONTAINED IN AQUEOUS EXTRACTS AND FRACTIONS OBTAINED FROM LEAVES OF Ugni Molinae TO DETERMINE THEIR EFFECT ON THE VIABILITY OF HUMAN GASTRIC CANCER CELLS
description	ABSTRACT Ugni molinae Turcz. Myrtaceae (Murtilla) is a plant native to Chile. There is evidence about the antiproliferative compounds present in fruits, but there is no evidence on the antiproliferative activity of the compounds in the leaves. These compounds have importance because they predominate in the popular form of ingestion of leaves. In the present study, the identification of water-soluble components of leaves of U. molinae and a fractionation were carried out to determine the effect on viability of gastric adenocarcinoma cells (AGS). A decoct of leaves was performed at 1%. For the separation of fractions, a countercurrent system (CPC) was used. Leaves were collected and identified by thinlayer chromatography and liquid chromatography-mass spectrometry (HPLC MS/MS). Samples were evaluated to determine the viability of AGS cells by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidefor (MTT) assays. Three large fractions were identified. In the first one, the phenolic compounds of medium polarity and low molecular weight (phenolic acids) are found. In The second fraction shows low concentrations of phenolic compounds with greater molecular weight (glycosylated and gallolylated flavonoids); and the third, has a predominance of hydrolysable tannins, procianidins and other highly hydrophilic compounds. About cell viability, it was found that, the 1% extract is the one that presented the best effects on cell viability inhibition, in comparation to tannin and flavonoid fractions. In conclusion, there is synergy in the compounds present in the aqueous extract, which causes greater inhibition of cell viability, unlike the separated fractions.
author	Avello,M Pastene,E Eillen,Torres
author_facet	Avello,M Pastene,E Eillen,Torres
author_sort	Avello,M
title	IDENTIFICATION OF WATER-SOLUBLE COMPOUNDS CONTAINED IN AQUEOUS EXTRACTS AND FRACTIONS OBTAINED FROM LEAVES OF Ugni Molinae TO DETERMINE THEIR EFFECT ON THE VIABILITY OF HUMAN GASTRIC CANCER CELLS
title_short	IDENTIFICATION OF WATER-SOLUBLE COMPOUNDS CONTAINED IN AQUEOUS EXTRACTS AND FRACTIONS OBTAINED FROM LEAVES OF Ugni Molinae TO DETERMINE THEIR EFFECT ON THE VIABILITY OF HUMAN GASTRIC CANCER CELLS
title_full	IDENTIFICATION OF WATER-SOLUBLE COMPOUNDS CONTAINED IN AQUEOUS EXTRACTS AND FRACTIONS OBTAINED FROM LEAVES OF Ugni Molinae TO DETERMINE THEIR EFFECT ON THE VIABILITY OF HUMAN GASTRIC CANCER CELLS
title_fullStr	IDENTIFICATION OF WATER-SOLUBLE COMPOUNDS CONTAINED IN AQUEOUS EXTRACTS AND FRACTIONS OBTAINED FROM LEAVES OF Ugni Molinae TO DETERMINE THEIR EFFECT ON THE VIABILITY OF HUMAN GASTRIC CANCER CELLS
title_full_unstemmed	IDENTIFICATION OF WATER-SOLUBLE COMPOUNDS CONTAINED IN AQUEOUS EXTRACTS AND FRACTIONS OBTAINED FROM LEAVES OF Ugni Molinae TO DETERMINE THEIR EFFECT ON THE VIABILITY OF HUMAN GASTRIC CANCER CELLS
title_sort	identification of water-soluble compounds contained in aqueous extracts and fractions obtained from leaves of ugni molinae to determine their effect on the viability of human gastric cancer cells
publisher	Sociedad Chilena de Química
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072020000204849
work_keys_str_mv	AT avellom identificationofwatersolublecompoundscontainedinaqueousextractsandfractionsobtainedfromleavesofugnimolinaetodeterminetheireffectontheviabilityofhumangastriccancercells AT pastenee identificationofwatersolublecompoundscontainedinaqueousextractsandfractionsobtainedfromleavesofugnimolinaetodeterminetheireffectontheviabilityofhumangastriccancercells AT eillentorres identificationofwatersolublecompoundscontainedinaqueousextractsandfractionsobtainedfromleavesofugnimolinaetodeterminetheireffectontheviabilityofhumangastriccancercells
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IDENTIFICATION OF WATER-SOLUBLE COMPOUNDS CONTAINED IN AQUEOUS EXTRACTS AND FRACTIONS OBTAINED FROM LEAVES OF Ugni Molinae TO DETERMINE THEIR EFFECT ON THE VIABILITY OF HUMAN GASTRIC CANCER CELLS

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