DEVELOPMENT OF A SIMPLE LC METHOD FOR QUANTIFICATION OF LAMOTRIGINE IN HUMAN SERUM

ABSTRACT A liquid chromatographic (LC) method for quantitative analysis of lamotrigine in human serum was developed using liquid –liquid extraction with ethyl acetate. Quantitation was achieved over the concentration range of 1.0 to 40.0 µg/mL (r = 0.999), using a mixture of acetonitrile:...

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Autores principales:	Albornoz P.,M., Mennickent C.,S., de Diego G.,M., Ríos G.,G.
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2020
Materias:	Lamotrigine, antiepileptics human serum liquid chromatography
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072020000404962
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spelling	oai:scielo:S0717-970720200004049622020-12-03DEVELOPMENT OF A SIMPLE LC METHOD FOR QUANTIFICATION OF LAMOTRIGINE IN HUMAN SERUMAlbornoz P.,M.Mennickent C.,S.de Diego G.,M.Ríos G.,G. Lamotrigine, antiepileptics human serum liquid chromatography ABSTRACT A liquid chromatographic (LC) method for quantitative analysis of lamotrigine in human serum was developed using liquid –liquid extraction with ethyl acetate. Quantitation was achieved over the concentration range of 1.0 to 40.0 µg/mL (r = 0.999), using a mixture of acetonitrile: phosphate buffer (0.5 M) of pH 4.5 (69:31 v/v) as mobile phase, with a flow of 1 mL min− 1. Column was C18 (150 mm x 4.6 mm, 5 cm; Merck), chloramphenicol was used as internal standard, and UV detection at α 306 nm. The intra-assay variation was between 1.22 % and 1.85 % and the inter-assay was between 1.72 % and 2.91 %. The detection limit was 0.14 µg/mL, and the quantification limit was 0.42 µg/mL. The method proved to be accurate, with a recovery between 94.02 % and 109.95 %, with RSD not higher than 2.91 % and was selective for lamotrigine (Rs between lamotrigine and chloramphenicol was 4.9). This method was successfully applied to quantify lamotrigine in patient serum samples. In conclusion, the method is precise, accurate, reproducible and selective for the analysis of lamotrigine in human serum. Therefore, it could be an important tool to evaluate drug level in this matrix and, of this way, to obtain a better drug effect.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.65 n.4 20202020-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072020000404962en10.4067/S0717-97072020000404962
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Lamotrigine, antiepileptics human serum liquid chromatography
spellingShingle	Lamotrigine, antiepileptics human serum liquid chromatography Albornoz P.,M. Mennickent C.,S. de Diego G.,M. Ríos G.,G. DEVELOPMENT OF A SIMPLE LC METHOD FOR QUANTIFICATION OF LAMOTRIGINE IN HUMAN SERUM
description	ABSTRACT A liquid chromatographic (LC) method for quantitative analysis of lamotrigine in human serum was developed using liquid –liquid extraction with ethyl acetate. Quantitation was achieved over the concentration range of 1.0 to 40.0 µg/mL (r = 0.999), using a mixture of acetonitrile: phosphate buffer (0.5 M) of pH 4.5 (69:31 v/v) as mobile phase, with a flow of 1 mL min− 1. Column was C18 (150 mm x 4.6 mm, 5 cm; Merck), chloramphenicol was used as internal standard, and UV detection at α 306 nm. The intra-assay variation was between 1.22 % and 1.85 % and the inter-assay was between 1.72 % and 2.91 %. The detection limit was 0.14 µg/mL, and the quantification limit was 0.42 µg/mL. The method proved to be accurate, with a recovery between 94.02 % and 109.95 %, with RSD not higher than 2.91 % and was selective for lamotrigine (Rs between lamotrigine and chloramphenicol was 4.9). This method was successfully applied to quantify lamotrigine in patient serum samples. In conclusion, the method is precise, accurate, reproducible and selective for the analysis of lamotrigine in human serum. Therefore, it could be an important tool to evaluate drug level in this matrix and, of this way, to obtain a better drug effect.
author	Albornoz P.,M. Mennickent C.,S. de Diego G.,M. Ríos G.,G.
author_facet	Albornoz P.,M. Mennickent C.,S. de Diego G.,M. Ríos G.,G.
author_sort	Albornoz P.,M.
title	DEVELOPMENT OF A SIMPLE LC METHOD FOR QUANTIFICATION OF LAMOTRIGINE IN HUMAN SERUM
title_short	DEVELOPMENT OF A SIMPLE LC METHOD FOR QUANTIFICATION OF LAMOTRIGINE IN HUMAN SERUM
title_full	DEVELOPMENT OF A SIMPLE LC METHOD FOR QUANTIFICATION OF LAMOTRIGINE IN HUMAN SERUM
title_fullStr	DEVELOPMENT OF A SIMPLE LC METHOD FOR QUANTIFICATION OF LAMOTRIGINE IN HUMAN SERUM
title_full_unstemmed	DEVELOPMENT OF A SIMPLE LC METHOD FOR QUANTIFICATION OF LAMOTRIGINE IN HUMAN SERUM
title_sort	development of a simple lc method for quantification of lamotrigine in human serum
publisher	Sociedad Chilena de Química
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072020000404962
work_keys_str_mv	AT albornozpm developmentofasimplelcmethodforquantificationoflamotrigineinhumanserum AT mennickentcs developmentofasimplelcmethodforquantificationoflamotrigineinhumanserum AT dediegogm developmentofasimplelcmethodforquantificationoflamotrigineinhumanserum AT riosgg developmentofasimplelcmethodforquantificationoflamotrigineinhumanserum
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DEVELOPMENT OF A SIMPLE LC METHOD FOR QUANTIFICATION OF LAMOTRIGINE IN HUMAN SERUM

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