VIBRATIONAL SPECTRUM CHARACTERIZATION OF OUTER SURFACE OF HELICOBACTER PYLORI BIOFILMS BY FUNCTIONALLY-ENHANCED DERIVATIVE SPECTROSCOPY (FEDS)

ABSTRACT Mid-infrared spectroscopy in conjunction with Functionally-Enhanced Derivative Spectroscopy (IR+FEDS) is a powerful analytical tool for the improvement of analysis of microorganism IR spectra. The objective of this research is to characterize the outer surface of two Helicobacter pylori str...

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Autores principales:	Palencia,Sixta L., García,Apolinaria, Palencia,Manuel
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2020
Materias:	Helicobacter pylori infrared spectrum biofilm FEDS transform surface sensing
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072020000405015
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spelling	oai:scielo:S0717-970720200004050152020-12-03VIBRATIONAL SPECTRUM CHARACTERIZATION OF OUTER SURFACE OF HELICOBACTER PYLORI BIOFILMS BY FUNCTIONALLY-ENHANCED DERIVATIVE SPECTROSCOPY (FEDS)Palencia,Sixta L.García,ApolinariaPalencia,Manuel Helicobacter pylori infrared spectrum biofilm FEDS transform surface sensing ABSTRACT Mid-infrared spectroscopy in conjunction with Functionally-Enhanced Derivative Spectroscopy (IR+FEDS) is a powerful analytical tool for the improvement of analysis of microorganism IR spectra. The objective of this research is to characterize the outer surface of two Helicobacter pylori strains by IR+FEDS. This work is a key stage for the study of cell-cell and cell-surface interactions between microorganisms, as well as, for polymicrobial biofilm characterizations where H. pylori species are involved. Artificial bacterial biofilms were deposited on ultrafiltration cellulose membranes covalently modified by insertion of one spectral marker and used as sensing surface for analysis of bacterial biolayers. Biolayers were analyzed using an infrared spectrophotometer with ATR. Data were analyzed by classic procedures and by deconvolution based on FEDS transform. It is concluded that, for correct application of technique is required a minimum amount of noise in the working spectra which can be achieved by simple smoothing algorithms; in addition, reproducibility must be warranted by the implementation of standardized protocols and the use of an appropriate number of samples. It is concluded that in addition to typical signals associated with the IR spectrum of microorganisms, by FEDS, a better and more detailed description of the outer membrane of H. pylori biofilms can be performed. In particular, it is concluded that the detecting and monitoring of cysteine-rich proteins can be satisfactorily performed by IR+FEDS.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.65 n.4 20202020-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072020000405015en10.4067/S0717-97072020000405015
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Helicobacter pylori infrared spectrum biofilm FEDS transform surface sensing
spellingShingle	Helicobacter pylori infrared spectrum biofilm FEDS transform surface sensing Palencia,Sixta L. García,Apolinaria Palencia,Manuel VIBRATIONAL SPECTRUM CHARACTERIZATION OF OUTER SURFACE OF HELICOBACTER PYLORI BIOFILMS BY FUNCTIONALLY-ENHANCED DERIVATIVE SPECTROSCOPY (FEDS)
description	ABSTRACT Mid-infrared spectroscopy in conjunction with Functionally-Enhanced Derivative Spectroscopy (IR+FEDS) is a powerful analytical tool for the improvement of analysis of microorganism IR spectra. The objective of this research is to characterize the outer surface of two Helicobacter pylori strains by IR+FEDS. This work is a key stage for the study of cell-cell and cell-surface interactions between microorganisms, as well as, for polymicrobial biofilm characterizations where H. pylori species are involved. Artificial bacterial biofilms were deposited on ultrafiltration cellulose membranes covalently modified by insertion of one spectral marker and used as sensing surface for analysis of bacterial biolayers. Biolayers were analyzed using an infrared spectrophotometer with ATR. Data were analyzed by classic procedures and by deconvolution based on FEDS transform. It is concluded that, for correct application of technique is required a minimum amount of noise in the working spectra which can be achieved by simple smoothing algorithms; in addition, reproducibility must be warranted by the implementation of standardized protocols and the use of an appropriate number of samples. It is concluded that in addition to typical signals associated with the IR spectrum of microorganisms, by FEDS, a better and more detailed description of the outer membrane of H. pylori biofilms can be performed. In particular, it is concluded that the detecting and monitoring of cysteine-rich proteins can be satisfactorily performed by IR+FEDS.
author	Palencia,Sixta L. García,Apolinaria Palencia,Manuel
author_facet	Palencia,Sixta L. García,Apolinaria Palencia,Manuel
author_sort	Palencia,Sixta L.
title	VIBRATIONAL SPECTRUM CHARACTERIZATION OF OUTER SURFACE OF HELICOBACTER PYLORI BIOFILMS BY FUNCTIONALLY-ENHANCED DERIVATIVE SPECTROSCOPY (FEDS)
title_short	VIBRATIONAL SPECTRUM CHARACTERIZATION OF OUTER SURFACE OF HELICOBACTER PYLORI BIOFILMS BY FUNCTIONALLY-ENHANCED DERIVATIVE SPECTROSCOPY (FEDS)
title_full	VIBRATIONAL SPECTRUM CHARACTERIZATION OF OUTER SURFACE OF HELICOBACTER PYLORI BIOFILMS BY FUNCTIONALLY-ENHANCED DERIVATIVE SPECTROSCOPY (FEDS)
title_fullStr	VIBRATIONAL SPECTRUM CHARACTERIZATION OF OUTER SURFACE OF HELICOBACTER PYLORI BIOFILMS BY FUNCTIONALLY-ENHANCED DERIVATIVE SPECTROSCOPY (FEDS)
title_full_unstemmed	VIBRATIONAL SPECTRUM CHARACTERIZATION OF OUTER SURFACE OF HELICOBACTER PYLORI BIOFILMS BY FUNCTIONALLY-ENHANCED DERIVATIVE SPECTROSCOPY (FEDS)
title_sort	vibrational spectrum characterization of outer surface of helicobacter pylori biofilms by functionally-enhanced derivative spectroscopy (feds)
publisher	Sociedad Chilena de Química
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072020000405015
work_keys_str_mv	AT palenciasixtal vibrationalspectrumcharacterizationofoutersurfaceofhelicobacterpyloribiofilmsbyfunctionallyenhancedderivativespectroscopyfeds AT garciaapolinaria vibrationalspectrumcharacterizationofoutersurfaceofhelicobacterpyloribiofilmsbyfunctionallyenhancedderivativespectroscopyfeds AT palenciamanuel vibrationalspectrumcharacterizationofoutersurfaceofhelicobacterpyloribiofilmsbyfunctionallyenhancedderivativespectroscopyfeds
_version_	1714200954115457024

VIBRATIONAL SPECTRUM CHARACTERIZATION OF OUTER SURFACE OF HELICOBACTER PYLORI BIOFILMS BY FUNCTIONALLY-ENHANCED DERIVATIVE SPECTROSCOPY (FEDS)

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