Mn-DOPPED ZnS QUANTUM DOTS AS SENSITIVE SENSOR FOR DETERMINATION OF CIPROFLOXACIN IN PHARMACEUTICAL AND BIOLOGICAL SAMPLES

ABSTRACT A simple and fast spectrofluorimetric method for determination of ciprofloxacin based on its quenching effect on the fluorescence intensity of colloidal water soluble TGA caped, Mn doped ZnS quantum dots (QDs) has been described. The QDs having characteristic fluorescence spectra with maxim...

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Autores principales:	Muhammad,Mian, Ara,Behisht, Ali,Faiz, Ahmad,Izaz, Ullah,Hafeez
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2021
Materias:	Spectrofluorimetric Ciprofloxacin Quenching Quantum dots Fluorescence intensity
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072021000205130
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spelling	oai:scielo:S0717-970720210002051302021-07-05Mn-DOPPED ZnS QUANTUM DOTS AS SENSITIVE SENSOR FOR DETERMINATION OF CIPROFLOXACIN IN PHARMACEUTICAL AND BIOLOGICAL SAMPLESMuhammad,MianAra,BehishtAli,FaizAhmad,IzazUllah,Hafeez Spectrofluorimetric Ciprofloxacin Quenching Quantum dots Fluorescence intensity ABSTRACT A simple and fast spectrofluorimetric method for determination of ciprofloxacin based on its quenching effect on the fluorescence intensity of colloidal water soluble TGA caped, Mn doped ZnS quantum dots (QDs) has been described. The QDs having characteristic fluorescence spectra with maximum excitation at 315 nm followed by an emission peak at 632 nm were characterized using Energy Dispersive X-Ray and X-ray Diffraction techniques. The effect of various parameters such as concentration of QDs, time, pH, common excipients and metal ions on the quenching phenomenon was investigated. Fluorescence quenching was found to be maximum with 10 µg mL-1 of QDs at pH 8 and fluorescence intensity was observed to be constant upto 45 min. The Stern-Volmer calibration plot of F0/F as a function of ciprofloxacin concentration was found to be linear in the range of 0.5-10 µg mL-1 with r² = 0.993. Under optimal experimental conditions, the method was found to be interference free. The percent relative standard deviation of the proposed method, calculated against method blank, was found to be 1.10%. The limit of detection (LOD) and the limit of quantitation (LOQ) for ciprofloxacin (signal to noise ratio 3:1 for LOD and 10:1 for LOQ) were calculated to be 0.15 and 0.50 μg mL-1 respectively. Average percent recoveries (±SD) obtained for spiked commercial formulations; ciprol and ciproquine, and serum and urine samples were found to be in the range of 92.2±1.8% to 99.5±1.5%, 90.5±1.2% to 96.7±1.4% and 94.0±1.2% to 98.5±1.4% respectively. The data evidently proves the potential of the proposed sensor QDs in biological analysis.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.66 n.2 20212021-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072021000205130en10.4067/S0717-97072021000205130
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Spectrofluorimetric Ciprofloxacin Quenching Quantum dots Fluorescence intensity
spellingShingle	Spectrofluorimetric Ciprofloxacin Quenching Quantum dots Fluorescence intensity Muhammad,Mian Ara,Behisht Ali,Faiz Ahmad,Izaz Ullah,Hafeez Mn-DOPPED ZnS QUANTUM DOTS AS SENSITIVE SENSOR FOR DETERMINATION OF CIPROFLOXACIN IN PHARMACEUTICAL AND BIOLOGICAL SAMPLES
description	ABSTRACT A simple and fast spectrofluorimetric method for determination of ciprofloxacin based on its quenching effect on the fluorescence intensity of colloidal water soluble TGA caped, Mn doped ZnS quantum dots (QDs) has been described. The QDs having characteristic fluorescence spectra with maximum excitation at 315 nm followed by an emission peak at 632 nm were characterized using Energy Dispersive X-Ray and X-ray Diffraction techniques. The effect of various parameters such as concentration of QDs, time, pH, common excipients and metal ions on the quenching phenomenon was investigated. Fluorescence quenching was found to be maximum with 10 µg mL-1 of QDs at pH 8 and fluorescence intensity was observed to be constant upto 45 min. The Stern-Volmer calibration plot of F0/F as a function of ciprofloxacin concentration was found to be linear in the range of 0.5-10 µg mL-1 with r² = 0.993. Under optimal experimental conditions, the method was found to be interference free. The percent relative standard deviation of the proposed method, calculated against method blank, was found to be 1.10%. The limit of detection (LOD) and the limit of quantitation (LOQ) for ciprofloxacin (signal to noise ratio 3:1 for LOD and 10:1 for LOQ) were calculated to be 0.15 and 0.50 μg mL-1 respectively. Average percent recoveries (±SD) obtained for spiked commercial formulations; ciprol and ciproquine, and serum and urine samples were found to be in the range of 92.2±1.8% to 99.5±1.5%, 90.5±1.2% to 96.7±1.4% and 94.0±1.2% to 98.5±1.4% respectively. The data evidently proves the potential of the proposed sensor QDs in biological analysis.
author	Muhammad,Mian Ara,Behisht Ali,Faiz Ahmad,Izaz Ullah,Hafeez
author_facet	Muhammad,Mian Ara,Behisht Ali,Faiz Ahmad,Izaz Ullah,Hafeez
author_sort	Muhammad,Mian
title	Mn-DOPPED ZnS QUANTUM DOTS AS SENSITIVE SENSOR FOR DETERMINATION OF CIPROFLOXACIN IN PHARMACEUTICAL AND BIOLOGICAL SAMPLES
title_short	Mn-DOPPED ZnS QUANTUM DOTS AS SENSITIVE SENSOR FOR DETERMINATION OF CIPROFLOXACIN IN PHARMACEUTICAL AND BIOLOGICAL SAMPLES
title_full	Mn-DOPPED ZnS QUANTUM DOTS AS SENSITIVE SENSOR FOR DETERMINATION OF CIPROFLOXACIN IN PHARMACEUTICAL AND BIOLOGICAL SAMPLES
title_fullStr	Mn-DOPPED ZnS QUANTUM DOTS AS SENSITIVE SENSOR FOR DETERMINATION OF CIPROFLOXACIN IN PHARMACEUTICAL AND BIOLOGICAL SAMPLES
title_full_unstemmed	Mn-DOPPED ZnS QUANTUM DOTS AS SENSITIVE SENSOR FOR DETERMINATION OF CIPROFLOXACIN IN PHARMACEUTICAL AND BIOLOGICAL SAMPLES
title_sort	mn-dopped zns quantum dots as sensitive sensor for determination of ciprofloxacin in pharmaceutical and biological samples
publisher	Sociedad Chilena de Química
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072021000205130
work_keys_str_mv	AT muhammadmian mndoppedznsquantumdotsassensitivesensorfordeterminationofciprofloxacininpharmaceuticalandbiologicalsamples AT arabehisht mndoppedznsquantumdotsassensitivesensorfordeterminationofciprofloxacininpharmaceuticalandbiologicalsamples AT alifaiz mndoppedznsquantumdotsassensitivesensorfordeterminationofciprofloxacininpharmaceuticalandbiologicalsamples AT ahmadizaz mndoppedznsquantumdotsassensitivesensorfordeterminationofciprofloxacininpharmaceuticalandbiologicalsamples AT ullahhafeez mndoppedznsquantumdotsassensitivesensorfordeterminationofciprofloxacininpharmaceuticalandbiologicalsamples
_version_	1714200957040984064

Mn-DOPPED ZnS QUANTUM DOTS AS SENSITIVE SENSOR FOR DETERMINATION OF CIPROFLOXACIN IN PHARMACEUTICAL AND BIOLOGICAL SAMPLES

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