PHYTOCHEMICAL ASSESSMENT, TOTAL PHENOLIC CONTENT, CYTOTOXIC, ANTIOXIDANT AND ANTIDIABETIC ACTIVITIES OF HYOSCYAMUS INSANUS

ABSTRACT Hyoscyamus insanus (family Solanaceae) was traditionally used for treatment of asthma, relieving pain and increasing female body weight. The present project was intended to assess the phytochemicals, total phenolic content, cytotoxic, antioxidant and antidiabetic activities of Hyoscyamus in...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Rehman-Shah,Muhammad Abdur, Khan,Rahmat Ali, Mushtaq,Ahmed
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2021
Materias:	Antidiabetic antioxidant cytotoxic Hyoscyamus insanus
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072021000305285
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-97072021000305285
record_format	dspace
spelling	oai:scielo:S0717-970720210003052852021-10-14PHYTOCHEMICAL ASSESSMENT, TOTAL PHENOLIC CONTENT, CYTOTOXIC, ANTIOXIDANT AND ANTIDIABETIC ACTIVITIES OF HYOSCYAMUS INSANUSRehman-Shah,Muhammad AbdurKhan,Rahmat AliMushtaq,Ahmed Antidiabetic antioxidant cytotoxic Hyoscyamus insanus ABSTRACT Hyoscyamus insanus (family Solanaceae) was traditionally used for treatment of asthma, relieving pain and increasing female body weight. The present project was intended to assess the phytochemicals, total phenolic content, cytotoxic, antioxidant and antidiabetic activities of Hyoscyamus insanus leaves methanolic extract and its special fractions. After extraction, the methanolic extract was sequentially fractionated with n -hexane, chloroform and water followed by phytochemicals analysis of each sample. Brine shrimp lethality bioassay, Folin-Ciocalteu phenol reagent, DPPH, ABTSo+, H2O2 and alpha-amylase inhibition assays were employed for the assessment of cytotoxic property, total phenolic content, antioxidant and anti-diabetic potency of methanolic extract and its fractions. Amino acids and protein, carbohydrates, flavonoids and saponins were found in the methanolic extract and aqueous fraction while chloroform fraction exhibited carbohydrates, flavonoids and saponins. The highest cytotoxic activity (80.6±1.2%) was exhibited by methanolic extract while maximum total phenolic content (21.93±1.17 mg GAE/g) were found in the chloroform fraction. The employed antioxidant assays expressed different results i.e. methanolic extract demonstrated highest; 83.99%, 74.19% and 51.15% free radical scavenging characteristics in ABTS, DPPH and H2O2 assays respectively. The methanolic extract showed significant (53.44%) antidiabetic properties. The correlation of total phenolic content with percentage antioxidant and anti-diabetic capabilities of methanolic extract and its special fractions was observed to be non-significant (P > 0.05). It is concluded that the Hyoscyamus insanus is a potential source of phenolic, cytotoxic, antioxidant and antidiabetic compounds.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.66 n.3 20212021-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072021000305285en10.4067/S0717-97072021000305285
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Antidiabetic antioxidant cytotoxic Hyoscyamus insanus
spellingShingle	Antidiabetic antioxidant cytotoxic Hyoscyamus insanus Rehman-Shah,Muhammad Abdur Khan,Rahmat Ali Mushtaq,Ahmed PHYTOCHEMICAL ASSESSMENT, TOTAL PHENOLIC CONTENT, CYTOTOXIC, ANTIOXIDANT AND ANTIDIABETIC ACTIVITIES OF HYOSCYAMUS INSANUS
description	ABSTRACT Hyoscyamus insanus (family Solanaceae) was traditionally used for treatment of asthma, relieving pain and increasing female body weight. The present project was intended to assess the phytochemicals, total phenolic content, cytotoxic, antioxidant and antidiabetic activities of Hyoscyamus insanus leaves methanolic extract and its special fractions. After extraction, the methanolic extract was sequentially fractionated with n -hexane, chloroform and water followed by phytochemicals analysis of each sample. Brine shrimp lethality bioassay, Folin-Ciocalteu phenol reagent, DPPH, ABTSo+, H2O2 and alpha-amylase inhibition assays were employed for the assessment of cytotoxic property, total phenolic content, antioxidant and anti-diabetic potency of methanolic extract and its fractions. Amino acids and protein, carbohydrates, flavonoids and saponins were found in the methanolic extract and aqueous fraction while chloroform fraction exhibited carbohydrates, flavonoids and saponins. The highest cytotoxic activity (80.6±1.2%) was exhibited by methanolic extract while maximum total phenolic content (21.93±1.17 mg GAE/g) were found in the chloroform fraction. The employed antioxidant assays expressed different results i.e. methanolic extract demonstrated highest; 83.99%, 74.19% and 51.15% free radical scavenging characteristics in ABTS, DPPH and H2O2 assays respectively. The methanolic extract showed significant (53.44%) antidiabetic properties. The correlation of total phenolic content with percentage antioxidant and anti-diabetic capabilities of methanolic extract and its special fractions was observed to be non-significant (P > 0.05). It is concluded that the Hyoscyamus insanus is a potential source of phenolic, cytotoxic, antioxidant and antidiabetic compounds.
author	Rehman-Shah,Muhammad Abdur Khan,Rahmat Ali Mushtaq,Ahmed
author_facet	Rehman-Shah,Muhammad Abdur Khan,Rahmat Ali Mushtaq,Ahmed
author_sort	Rehman-Shah,Muhammad Abdur
title	PHYTOCHEMICAL ASSESSMENT, TOTAL PHENOLIC CONTENT, CYTOTOXIC, ANTIOXIDANT AND ANTIDIABETIC ACTIVITIES OF HYOSCYAMUS INSANUS
title_short	PHYTOCHEMICAL ASSESSMENT, TOTAL PHENOLIC CONTENT, CYTOTOXIC, ANTIOXIDANT AND ANTIDIABETIC ACTIVITIES OF HYOSCYAMUS INSANUS
title_full	PHYTOCHEMICAL ASSESSMENT, TOTAL PHENOLIC CONTENT, CYTOTOXIC, ANTIOXIDANT AND ANTIDIABETIC ACTIVITIES OF HYOSCYAMUS INSANUS
title_fullStr	PHYTOCHEMICAL ASSESSMENT, TOTAL PHENOLIC CONTENT, CYTOTOXIC, ANTIOXIDANT AND ANTIDIABETIC ACTIVITIES OF HYOSCYAMUS INSANUS
title_full_unstemmed	PHYTOCHEMICAL ASSESSMENT, TOTAL PHENOLIC CONTENT, CYTOTOXIC, ANTIOXIDANT AND ANTIDIABETIC ACTIVITIES OF HYOSCYAMUS INSANUS
title_sort	phytochemical assessment, total phenolic content, cytotoxic, antioxidant and antidiabetic activities of hyoscyamus insanus
publisher	Sociedad Chilena de Química
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072021000305285
work_keys_str_mv	AT rehmanshahmuhammadabdur phytochemicalassessmenttotalphenoliccontentcytotoxicantioxidantandantidiabeticactivitiesofhyoscyamusinsanus AT khanrahmatali phytochemicalassessmenttotalphenoliccontentcytotoxicantioxidantandantidiabeticactivitiesofhyoscyamusinsanus AT mushtaqahmed phytochemicalassessmenttotalphenoliccontentcytotoxicantioxidantandantidiabeticactivitiesofhyoscyamusinsanus
_version_	1718324218863550464

PHYTOCHEMICAL ASSESSMENT, TOTAL PHENOLIC CONTENT, CYTOTOXIC, ANTIOXIDANT AND ANTIDIABETIC ACTIVITIES OF HYOSCYAMUS INSANUS

Ejemplares similares