Identification of proanthocyanidins extracted from Pinus radiata D. Don bark

The bark of Pinus radiata D. Don is recognized as a rich natural source of proanthocyanidins (PAs). Barks of Pinus radiata D. Don were extracted to produce a raw extract, and then purified to produce 4 fractions, with the aim of correlating its structural characteristics with the extract antioxidant...

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Autores principales:	Cortés,Soledad, Pulgar,Hugo, Sanhueza,Verónica, Aspé,Estrella, Fernández,Katherina
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal 2010
Materias:	Antioxidant activity bark Pinus radiata tannins
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202010000200002
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spelling	oai:scielo:S0718-162020100002000022010-10-14Identification of proanthocyanidins extracted from Pinus radiata D. Don barkCortés,SoledadPulgar,HugoSanhueza,VerónicaAspé,EstrellaFernández,Katherina Antioxidant activity bark Pinus radiata tannins The bark of Pinus radiata D. Don is recognized as a rich natural source of proanthocyanidins (PAs). Barks of Pinus radiata D. Don were extracted to produce a raw extract, and then purified to produce 4 fractions, with the aim of correlating its structural characteristics with the extract antioxidant capacity. The structural characterization was performed by acid catalysis in presence of phloroglucinol, foliowed by the detection of the components by High Precision Liquid Chromatography (HPLC). The subunits identified in the bark of Pinus radiata were: epicatechin- (4ß-2) - phloroglucinol (EC-P), catechin- (4á-2) - phloroglucinol (C-F) and (+) -catechin (C). The purified fractions showed different mean degree of polymerization (mDP), average molecular weight (aMW), concentration and reaction yield (R) with phloroglucinol. The concentration of total phenols decreased according to the order in which the fractions were eluted. All the fractions presented a high antiradical activity (evaluated as scavenging capacity of radical DPPH>). Nevertheless, the fraction FU presented the greatest tannin concentration and antioxidant activity. The structural characteristics of the extract of Pinus radiata D. Don bark could be correlated with the antioxidant activity of the extract.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería ForestalCiencia e investigación agraria v.37 n.2 20102010-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202010000200002en10.4067/S0718-16202010000200002
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Antioxidant activity bark Pinus radiata tannins
spellingShingle	Antioxidant activity bark Pinus radiata tannins Cortés,Soledad Pulgar,Hugo Sanhueza,Verónica Aspé,Estrella Fernández,Katherina Identification of proanthocyanidins extracted from Pinus radiata D. Don bark
description	The bark of Pinus radiata D. Don is recognized as a rich natural source of proanthocyanidins (PAs). Barks of Pinus radiata D. Don were extracted to produce a raw extract, and then purified to produce 4 fractions, with the aim of correlating its structural characteristics with the extract antioxidant capacity. The structural characterization was performed by acid catalysis in presence of phloroglucinol, foliowed by the detection of the components by High Precision Liquid Chromatography (HPLC). The subunits identified in the bark of Pinus radiata were: epicatechin- (4ß-2) - phloroglucinol (EC-P), catechin- (4á-2) - phloroglucinol (C-F) and (+) -catechin (C). The purified fractions showed different mean degree of polymerization (mDP), average molecular weight (aMW), concentration and reaction yield (R) with phloroglucinol. The concentration of total phenols decreased according to the order in which the fractions were eluted. All the fractions presented a high antiradical activity (evaluated as scavenging capacity of radical DPPH>). Nevertheless, the fraction FU presented the greatest tannin concentration and antioxidant activity. The structural characteristics of the extract of Pinus radiata D. Don bark could be correlated with the antioxidant activity of the extract.
author	Cortés,Soledad Pulgar,Hugo Sanhueza,Verónica Aspé,Estrella Fernández,Katherina
author_facet	Cortés,Soledad Pulgar,Hugo Sanhueza,Verónica Aspé,Estrella Fernández,Katherina
author_sort	Cortés,Soledad
title	Identification of proanthocyanidins extracted from Pinus radiata D. Don bark
title_short	Identification of proanthocyanidins extracted from Pinus radiata D. Don bark
title_full	Identification of proanthocyanidins extracted from Pinus radiata D. Don bark
title_fullStr	Identification of proanthocyanidins extracted from Pinus radiata D. Don bark
title_full_unstemmed	Identification of proanthocyanidins extracted from Pinus radiata D. Don bark
title_sort	identification of proanthocyanidins extracted from pinus radiata d. don bark
publisher	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202010000200002
work_keys_str_mv	AT cortessoledad identificationofproanthocyanidinsextractedfrompinusradiataddonbark AT pulgarhugo identificationofproanthocyanidinsextractedfrompinusradiataddonbark AT sanhuezaveronica identificationofproanthocyanidinsextractedfrompinusradiataddonbark AT aspeestrella identificationofproanthocyanidinsextractedfrompinusradiataddonbark AT fernandezkatherina identificationofproanthocyanidinsextractedfrompinusradiataddonbark
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Identification of proanthocyanidins extracted from Pinus radiata D. Don bark

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