Effect of Chilean propolis on cariogenic bacteria Lactobacillus fermentum

Dental caries is an infectious disease of worldwide public health concern. Among the bacteria involved in this pathology are Streptococcus mutans, Streptococcus sobrinus and organisms belonging to the genera Actinomyces and Lactobacillus. The pharmaceutical industry is focussing on the discovery of...

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Autores principales:	Saavedra,Nicolás, Barrientos,Leticia, Herrera,Christian L, Alvear,Marysol, Montenegro,Gloria, Salazar,Luis A
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal 2011
Materias:	Lactobacillus fermentum dental caries propolis antibacterial activity
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202011000100011
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spelling	oai:scielo:S0718-162020110001000112011-08-18Effect of Chilean propolis on cariogenic bacteria Lactobacillus fermentumSaavedra,NicolásBarrientos,LeticiaHerrera,Christian LAlvear,MarysolMontenegro,GloriaSalazar,Luis A Lactobacillus fermentum dental caries propolis antibacterial activity Dental caries is an infectious disease of worldwide public health concern. Among the bacteria involved in this pathology are Streptococcus mutans, Streptococcus sobrinus and organisms belonging to the genera Actinomyces and Lactobacillus. The pharmaceutical industry is focussing on the discovery of new antibacterial products after a greater resistance to those already known. Propolis has been used since ancient times, so their effects against various microorganisms have been already investigated. In our study, we evaluated the antimicrobial effect of 6 commercial ethanolic propolis extracts on the bacterium Lactobacillus fermentum. This bacterium was isolated after its identification by Polymerase Chain Reaction using species specific primers, and after growing microbiological samples from cavities of patients diagnosed with dental caries and with indication of tooth extraction. L. fermentum was detected in 9 of 40 patients, corresponding to 22%. The susceptibility study, carried out by microplate dilution, found antimicrobial activity in four of the six ethanolic extract of propolis used. These differ in the effective concentration against the microorganism, which can be attributed to factors such as the botanical origin, geographic location and harvest season. Among the results, it was noticed that these polyphenols showed concentrations ranging between 9 ± 0.3 and 85 ±2.1 mg/mL. The chromatographic analysis allowed the identification of caffeic acid, myricetin, quercetin, kaempherol, apigenin, pinocembrin, galangin and caffeic acid phenethyl ester (CAPE). Our study demonstrates the antimicrobial action of propolis on L. fermentum, the patogen related to caries development.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería ForestalCiencia e investigación agraria v.38 n.1 20112011-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202011000100011en10.4067/S0718-16202011000100011
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Lactobacillus fermentum dental caries propolis antibacterial activity
spellingShingle	Lactobacillus fermentum dental caries propolis antibacterial activity Saavedra,Nicolás Barrientos,Leticia Herrera,Christian L Alvear,Marysol Montenegro,Gloria Salazar,Luis A Effect of Chilean propolis on cariogenic bacteria Lactobacillus fermentum
description	Dental caries is an infectious disease of worldwide public health concern. Among the bacteria involved in this pathology are Streptococcus mutans, Streptococcus sobrinus and organisms belonging to the genera Actinomyces and Lactobacillus. The pharmaceutical industry is focussing on the discovery of new antibacterial products after a greater resistance to those already known. Propolis has been used since ancient times, so their effects against various microorganisms have been already investigated. In our study, we evaluated the antimicrobial effect of 6 commercial ethanolic propolis extracts on the bacterium Lactobacillus fermentum. This bacterium was isolated after its identification by Polymerase Chain Reaction using species specific primers, and after growing microbiological samples from cavities of patients diagnosed with dental caries and with indication of tooth extraction. L. fermentum was detected in 9 of 40 patients, corresponding to 22%. The susceptibility study, carried out by microplate dilution, found antimicrobial activity in four of the six ethanolic extract of propolis used. These differ in the effective concentration against the microorganism, which can be attributed to factors such as the botanical origin, geographic location and harvest season. Among the results, it was noticed that these polyphenols showed concentrations ranging between 9 ± 0.3 and 85 ±2.1 mg/mL. The chromatographic analysis allowed the identification of caffeic acid, myricetin, quercetin, kaempherol, apigenin, pinocembrin, galangin and caffeic acid phenethyl ester (CAPE). Our study demonstrates the antimicrobial action of propolis on L. fermentum, the patogen related to caries development.
author	Saavedra,Nicolás Barrientos,Leticia Herrera,Christian L Alvear,Marysol Montenegro,Gloria Salazar,Luis A
author_facet	Saavedra,Nicolás Barrientos,Leticia Herrera,Christian L Alvear,Marysol Montenegro,Gloria Salazar,Luis A
author_sort	Saavedra,Nicolás
title	Effect of Chilean propolis on cariogenic bacteria Lactobacillus fermentum
title_short	Effect of Chilean propolis on cariogenic bacteria Lactobacillus fermentum
title_full	Effect of Chilean propolis on cariogenic bacteria Lactobacillus fermentum
title_fullStr	Effect of Chilean propolis on cariogenic bacteria Lactobacillus fermentum
title_full_unstemmed	Effect of Chilean propolis on cariogenic bacteria Lactobacillus fermentum
title_sort	effect of chilean propolis on cariogenic bacteria lactobacillus fermentum
publisher	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202011000100011
work_keys_str_mv	AT saavedranicolas effectofchileanpropolisoncariogenicbacterialactobacillusfermentum AT barrientosleticia effectofchileanpropolisoncariogenicbacterialactobacillusfermentum AT herrerachristianl effectofchileanpropolisoncariogenicbacterialactobacillusfermentum AT alvearmarysol effectofchileanpropolisoncariogenicbacterialactobacillusfermentum AT montenegrogloria effectofchileanpropolisoncariogenicbacterialactobacillusfermentum AT salazarluisa effectofchileanpropolisoncariogenicbacterialactobacillusfermentum
_version_	1714202129878482944

Effect of Chilean propolis on cariogenic bacteria Lactobacillus fermentum

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