Genetic variation among and within tritipyrum (Thinopyrum bessarabicum x Triticum durum) lines using PCR-based molecular markers

Primary hexaploid tritipyrum lines are amphiploids between Triticum durum and Thinopyrum bessarabicum, which can set seed in at least 250 mM NaCl. Genetic variation within and among 14 lines of primary tritipyrum were investigated by using 2 random and 6 semi random primers that produced clear and r...

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Autores principales:	Siahsar,Barat Ali, Allahdoo,Maryam, Shahsevand Hassani,Hossein
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal 2011
Materias:	Aneuploidy genetic variation random primers semi random primers tritipyrum lines
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202011000100012
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spelling	oai:scielo:S0718-162020110001000122011-08-18Genetic variation among and within tritipyrum (Thinopyrum bessarabicum x Triticum durum) lines using PCR-based molecular markersSiahsar,Barat AliAllahdoo,MaryamShahsevand Hassani,Hossein Aneuploidy genetic variation random primers semi random primers tritipyrum lines Primary hexaploid tritipyrum lines are amphiploids between Triticum durum and Thinopyrum bessarabicum, which can set seed in at least 250 mM NaCl. Genetic variation within and among 14 lines of primary tritipyrum were investigated by using 2 random and 6 semi random primers that produced clear and reproducible DNA amplicons. Average values for Nei's gene diversity (h) and Shannon indices (I) were 0.115 and 0.178, respectively. These results indicated low variation within primary tritipyrum lines, probably related to unbiased chromosome and partial aneuploidy. Total genetic diversity (Ht) and gene diversity within lines (Hs) were 0.2114 and 0.1156, respectively. The coefficient of genetic differentiation (Gst) was equal to 0.4534. AMOVA showed highly significant (P<0.001) genetic differences among lines. From the total genetic diversity, 51.54% is attributable to 14 tritipyrum lines and 48.16% to differences within lines. Fixation index (FST) was 0.4815. Genetic distance, calculated on the basis of allele frequencies among lines, ranged from 0.064 to 0.226 with an average of 0.1156. Grouping of populations based on UPGMA and Nei's gene diversity are indications of genetic similarity between some populations. Pop6 and populations located in group III had the highest genetic distance. These results could be used in breeding and cross programs to breed a new tritipyrum plant.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería ForestalCiencia e investigación agraria v.38 n.1 20112011-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202011000100012en10.4067/S0718-16202011000100012
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Aneuploidy genetic variation random primers semi random primers tritipyrum lines
spellingShingle	Aneuploidy genetic variation random primers semi random primers tritipyrum lines Siahsar,Barat Ali Allahdoo,Maryam Shahsevand Hassani,Hossein Genetic variation among and within tritipyrum (Thinopyrum bessarabicum x Triticum durum) lines using PCR-based molecular markers
description	Primary hexaploid tritipyrum lines are amphiploids between Triticum durum and Thinopyrum bessarabicum, which can set seed in at least 250 mM NaCl. Genetic variation within and among 14 lines of primary tritipyrum were investigated by using 2 random and 6 semi random primers that produced clear and reproducible DNA amplicons. Average values for Nei's gene diversity (h) and Shannon indices (I) were 0.115 and 0.178, respectively. These results indicated low variation within primary tritipyrum lines, probably related to unbiased chromosome and partial aneuploidy. Total genetic diversity (Ht) and gene diversity within lines (Hs) were 0.2114 and 0.1156, respectively. The coefficient of genetic differentiation (Gst) was equal to 0.4534. AMOVA showed highly significant (P<0.001) genetic differences among lines. From the total genetic diversity, 51.54% is attributable to 14 tritipyrum lines and 48.16% to differences within lines. Fixation index (FST) was 0.4815. Genetic distance, calculated on the basis of allele frequencies among lines, ranged from 0.064 to 0.226 with an average of 0.1156. Grouping of populations based on UPGMA and Nei's gene diversity are indications of genetic similarity between some populations. Pop6 and populations located in group III had the highest genetic distance. These results could be used in breeding and cross programs to breed a new tritipyrum plant.
author	Siahsar,Barat Ali Allahdoo,Maryam Shahsevand Hassani,Hossein
author_facet	Siahsar,Barat Ali Allahdoo,Maryam Shahsevand Hassani,Hossein
author_sort	Siahsar,Barat Ali
title	Genetic variation among and within tritipyrum (Thinopyrum bessarabicum x Triticum durum) lines using PCR-based molecular markers
title_short	Genetic variation among and within tritipyrum (Thinopyrum bessarabicum x Triticum durum) lines using PCR-based molecular markers
title_full	Genetic variation among and within tritipyrum (Thinopyrum bessarabicum x Triticum durum) lines using PCR-based molecular markers
title_fullStr	Genetic variation among and within tritipyrum (Thinopyrum bessarabicum x Triticum durum) lines using PCR-based molecular markers
title_full_unstemmed	Genetic variation among and within tritipyrum (Thinopyrum bessarabicum x Triticum durum) lines using PCR-based molecular markers
title_sort	genetic variation among and within tritipyrum (thinopyrum bessarabicum x triticum durum) lines using pcr-based molecular markers
publisher	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202011000100012
work_keys_str_mv	AT siahsarbaratali geneticvariationamongandwithintritipyrumthinopyrumbessarabicumxtriticumdurumlinesusingpcrbasedmolecularmarkers AT allahdoomaryam geneticvariationamongandwithintritipyrumthinopyrumbessarabicumxtriticumdurumlinesusingpcrbasedmolecularmarkers AT shahsevandhassanihossein geneticvariationamongandwithintritipyrumthinopyrumbessarabicumxtriticumdurumlinesusingpcrbasedmolecularmarkers
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Genetic variation among and within tritipyrum (Thinopyrum bessarabicum x Triticum durum) lines using PCR-based molecular markers

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