Molecular characterization of Lotus corniculatus cultivars using transferable microsatellite markers

Lotus corniculatus L. is the most important agricultural species in the genus Lotus and is the most widely distributed Lotus species worldwide. L. corniculatus genotypes form complex groups that are difficult to recognize both morphologically and biochemically. Given the extensive and expensive proc...

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Autores principales:	Alem,Diego, Narancio,Rafael, Díaz Dellavalle,Paola, Rebuffo,Mónica, Zarza,Rodrigo, Dalla Rizza,Marco
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal 2011
Materias:	Genetic variability Lotus japonicus SSR
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202011000300015
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spelling	oai:scielo:S0718-162020110003000152012-01-23Molecular characterization of Lotus corniculatus cultivars using transferable microsatellite markersAlem,DiegoNarancio,RafaelDíaz Dellavalle,PaolaRebuffo,MónicaZarza,RodrigoDalla Rizza,Marco Genetic variability Lotus japonicus SSR Lotus corniculatus L. is the most important agricultural species in the genus Lotus and is the most widely distributed Lotus species worldwide. L. corniculatus genotypes form complex groups that are difficult to recognize both morphologically and biochemically. Given the extensive and expensive process of isolating Simple Sequence Repeats (SSR, also called microsatellites), the possibility of using microsatellites already identified in related species is highly attractive. The aim of this work was the identification and validation of transferable microsatellite markers in L. corniculatus, and using those markers to study the genetic variability among four cultivars. Each cultivar of L. corniculatus was represented by 15 genotypes. Ten microsatellite markers were evaluated, and from those, four were selected based on their discriminative values observed among cultivars. We detected 29 alleles for the four markers, and there was an average of 7.25 alleles per locus. The marker TM0197 had the fewest number of alleles (5) and TM0083 had the highest number of alleles (10). The polymorphic information content (PIC) for the selected markers varied from 0.19 to 0.35, and the markers were therefore classified as highly informative. Based on the markers, we found high variability between individuals of the same cultivar. The use of transferable microsatellite markers could be useful to differentiate individuals at a relatively low cost, showing a great potential for use in breeding programs.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería ForestalCiencia e investigación agraria v.38 n.3 20112011-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202011000300015en10.4067/S0718-16202011000300015
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Genetic variability Lotus japonicus SSR
spellingShingle	Genetic variability Lotus japonicus SSR Alem,Diego Narancio,Rafael Díaz Dellavalle,Paola Rebuffo,Mónica Zarza,Rodrigo Dalla Rizza,Marco Molecular characterization of Lotus corniculatus cultivars using transferable microsatellite markers
description	Lotus corniculatus L. is the most important agricultural species in the genus Lotus and is the most widely distributed Lotus species worldwide. L. corniculatus genotypes form complex groups that are difficult to recognize both morphologically and biochemically. Given the extensive and expensive process of isolating Simple Sequence Repeats (SSR, also called microsatellites), the possibility of using microsatellites already identified in related species is highly attractive. The aim of this work was the identification and validation of transferable microsatellite markers in L. corniculatus, and using those markers to study the genetic variability among four cultivars. Each cultivar of L. corniculatus was represented by 15 genotypes. Ten microsatellite markers were evaluated, and from those, four were selected based on their discriminative values observed among cultivars. We detected 29 alleles for the four markers, and there was an average of 7.25 alleles per locus. The marker TM0197 had the fewest number of alleles (5) and TM0083 had the highest number of alleles (10). The polymorphic information content (PIC) for the selected markers varied from 0.19 to 0.35, and the markers were therefore classified as highly informative. Based on the markers, we found high variability between individuals of the same cultivar. The use of transferable microsatellite markers could be useful to differentiate individuals at a relatively low cost, showing a great potential for use in breeding programs.
author	Alem,Diego Narancio,Rafael Díaz Dellavalle,Paola Rebuffo,Mónica Zarza,Rodrigo Dalla Rizza,Marco
author_facet	Alem,Diego Narancio,Rafael Díaz Dellavalle,Paola Rebuffo,Mónica Zarza,Rodrigo Dalla Rizza,Marco
author_sort	Alem,Diego
title	Molecular characterization of Lotus corniculatus cultivars using transferable microsatellite markers
title_short	Molecular characterization of Lotus corniculatus cultivars using transferable microsatellite markers
title_full	Molecular characterization of Lotus corniculatus cultivars using transferable microsatellite markers
title_fullStr	Molecular characterization of Lotus corniculatus cultivars using transferable microsatellite markers
title_full_unstemmed	Molecular characterization of Lotus corniculatus cultivars using transferable microsatellite markers
title_sort	molecular characterization of lotus corniculatus cultivars using transferable microsatellite markers
publisher	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202011000300015
work_keys_str_mv	AT alemdiego molecularcharacterizationoflotuscorniculatuscultivarsusingtransferablemicrosatellitemarkers AT naranciorafael molecularcharacterizationoflotuscorniculatuscultivarsusingtransferablemicrosatellitemarkers AT diazdellavallepaola molecularcharacterizationoflotuscorniculatuscultivarsusingtransferablemicrosatellitemarkers AT rebuffomonica molecularcharacterizationoflotuscorniculatuscultivarsusingtransferablemicrosatellitemarkers AT zarzarodrigo molecularcharacterizationoflotuscorniculatuscultivarsusingtransferablemicrosatellitemarkers AT dallarizzamarco molecularcharacterizationoflotuscorniculatuscultivarsusingtransferablemicrosatellitemarkers
_version_	1714202135580639232

Molecular characterization of Lotus corniculatus cultivars using transferable microsatellite markers

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