Effect of the inoculum size on the axenic in vitro production of Steinernema carpocapsae

The effect of inoculum size on the axenic in vitro production of Steinernema carpocapsae, using a transparent medium with hemoglobin and cholesterol, was studied. Axenic cultivation was performed with fertile eggs ofS. carpocapsae (hatching of eggs: 89 ± 4%), which were obtained by lyses of female n...

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Autores principales:	Suárez-Sánchez,Juan, Alatorre-Rosas,Raquel, Poggi-Varaldo,Héctor M, Barrera-Cortés,Josefina
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal 2012
Materias:	Axenic culture hemoglobin in vitro culture Steinernema carpocapsae transparent medium
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000100011
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spelling	oai:scielo:S0718-162020120001000112014-11-05Effect of the inoculum size on the axenic in vitro production of Steinernema carpocapsaeSuárez-Sánchez,JuanAlatorre-Rosas,RaquelPoggi-Varaldo,Héctor MBarrera-Cortés,Josefina Axenic culture hemoglobin in vitro culture Steinernema carpocapsae transparent medium The effect of inoculum size on the axenic in vitro production of Steinernema carpocapsae, using a transparent medium with hemoglobin and cholesterol, was studied. Axenic cultivation was performed with fertile eggs ofS. carpocapsae (hatching of eggs: 89 ± 4%), which were obtained by lyses of female nematodes and subsequent incubation. Two important aspects of this work include the use of a completely transparent medium enriched with hemoglobin and cholesterol and the high multiplication factor of the population (MFIP) of 87, which is considered high for in vitro axenic cultures of nematodes. In vivo methods have the advantage of high MFIP, but they also have the disadvantage of the high number and complexity of the operations involved for the purposes of harvesting axenic nematodes. It is assumed that the availability of a high number of nematodes in a transparent medium will further encourage the characterization of new native strains of nematodes.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería ForestalCiencia e investigación agraria v.39 n.1 20122012-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000100011en10.4067/S0718-16202012000100011
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Axenic culture hemoglobin in vitro culture Steinernema carpocapsae transparent medium
spellingShingle	Axenic culture hemoglobin in vitro culture Steinernema carpocapsae transparent medium Suárez-Sánchez,Juan Alatorre-Rosas,Raquel Poggi-Varaldo,Héctor M Barrera-Cortés,Josefina Effect of the inoculum size on the axenic in vitro production of Steinernema carpocapsae
description	The effect of inoculum size on the axenic in vitro production of Steinernema carpocapsae, using a transparent medium with hemoglobin and cholesterol, was studied. Axenic cultivation was performed with fertile eggs ofS. carpocapsae (hatching of eggs: 89 ± 4%), which were obtained by lyses of female nematodes and subsequent incubation. Two important aspects of this work include the use of a completely transparent medium enriched with hemoglobin and cholesterol and the high multiplication factor of the population (MFIP) of 87, which is considered high for in vitro axenic cultures of nematodes. In vivo methods have the advantage of high MFIP, but they also have the disadvantage of the high number and complexity of the operations involved for the purposes of harvesting axenic nematodes. It is assumed that the availability of a high number of nematodes in a transparent medium will further encourage the characterization of new native strains of nematodes.
author	Suárez-Sánchez,Juan Alatorre-Rosas,Raquel Poggi-Varaldo,Héctor M Barrera-Cortés,Josefina
author_facet	Suárez-Sánchez,Juan Alatorre-Rosas,Raquel Poggi-Varaldo,Héctor M Barrera-Cortés,Josefina
author_sort	Suárez-Sánchez,Juan
title	Effect of the inoculum size on the axenic in vitro production of Steinernema carpocapsae
title_short	Effect of the inoculum size on the axenic in vitro production of Steinernema carpocapsae
title_full	Effect of the inoculum size on the axenic in vitro production of Steinernema carpocapsae
title_fullStr	Effect of the inoculum size on the axenic in vitro production of Steinernema carpocapsae
title_full_unstemmed	Effect of the inoculum size on the axenic in vitro production of Steinernema carpocapsae
title_sort	effect of the inoculum size on the axenic in vitro production of steinernema carpocapsae
publisher	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000100011
work_keys_str_mv	AT suarezsanchezjuan effectoftheinoculumsizeontheaxenicinvitroproductionofsteinernemacarpocapsae AT alatorrerosasraquel effectoftheinoculumsizeontheaxenicinvitroproductionofsteinernemacarpocapsae AT poggivaraldohectorm effectoftheinoculumsizeontheaxenicinvitroproductionofsteinernemacarpocapsae AT barreracortesjosefina effectoftheinoculumsizeontheaxenicinvitroproductionofsteinernemacarpocapsae
_version_	1714202137422987264

Effect of the inoculum size on the axenic in vitro production of Steinernema carpocapsae

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