Molecular detection and classification of the phytoplasma that causes purple top in potatoes (Solanum tuberosum) in the State of Mexico

The Mexican potato (Solanum tuberosum L.) crop is affected by several diseases, including purple top (PPT). PPT is caused by phytoplasma and is transmitted by insects in the Cicadellidae family. In severe infections, this pathogen can cause yield losses of nearly 100%. The objective of this research...

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Autores principales:	Gutiérrez-Ibáñez,Ana T, Laguna-Cerda,Antonio, Rojas-Martínez,Reina, González-Garza,Ramiro, Salgado-Siclán,Martha L
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal 2012
Materias:	Phytoplasma potato diseases potato purple top
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000200010
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spelling	oai:scielo:S0718-162020120002000102012-10-24Molecular detection and classification of the phytoplasma that causes purple top in potatoes (Solanum tuberosum) in the State of MexicoGutiérrez-Ibáñez,Ana TLaguna-Cerda,AntonioRojas-Martínez,ReinaGonzález-Garza,RamiroSalgado-Siclán,Martha L Phytoplasma potato diseases potato purple top The Mexican potato (Solanum tuberosum L.) crop is affected by several diseases, including purple top (PPT). PPT is caused by phytoplasma and is transmitted by insects in the Cicadellidae family. In severe infections, this pathogen can cause yield losses of nearly 100%. The objective of this research was to identify and classify (at a molecular level) the causal agents of PPT. Potato plants were sampled in the state of Mexico (at Tenango del Valle, Zinacantepec, Atlacomulco and Donato Guerra) in the spring and summer of 2006. Approximately 40% of the plants tested positive for phytoplasma. The presence of plant DNA was determined by amplifying an endogenous gene with the 16s-1/16s-2 primers. To detect phytoplasma, the P1/P7 universal primers were used for PCR, the R16F2n/R16R2 primers were used for nested PCR, and the AluI, HpaII, and KpnI enzymes were used to perform RFLPs. The results indicated that the phytoplasma associated with the PPT disease belongs to the aster yellows (16Sr1) group and the I-B subgroup. The same phytoplasma was found in all three regions of study. When the sequence of the amplified fragment was compared with the registered Gene Bank (NCBI) sequences (using the BLAST package) a homology of 99% was found with the aster yellows (16Srl) group, "Candidatus Phytoplasma asteris".info:eu-repo/semantics/openAccessPontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería ForestalCiencia e investigación agraria v.39 n.2 20122012-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000200010en10.4067/S0718-16202012000200010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Phytoplasma potato diseases potato purple top
spellingShingle	Phytoplasma potato diseases potato purple top Gutiérrez-Ibáñez,Ana T Laguna-Cerda,Antonio Rojas-Martínez,Reina González-Garza,Ramiro Salgado-Siclán,Martha L Molecular detection and classification of the phytoplasma that causes purple top in potatoes (Solanum tuberosum) in the State of Mexico
description	The Mexican potato (Solanum tuberosum L.) crop is affected by several diseases, including purple top (PPT). PPT is caused by phytoplasma and is transmitted by insects in the Cicadellidae family. In severe infections, this pathogen can cause yield losses of nearly 100%. The objective of this research was to identify and classify (at a molecular level) the causal agents of PPT. Potato plants were sampled in the state of Mexico (at Tenango del Valle, Zinacantepec, Atlacomulco and Donato Guerra) in the spring and summer of 2006. Approximately 40% of the plants tested positive for phytoplasma. The presence of plant DNA was determined by amplifying an endogenous gene with the 16s-1/16s-2 primers. To detect phytoplasma, the P1/P7 universal primers were used for PCR, the R16F2n/R16R2 primers were used for nested PCR, and the AluI, HpaII, and KpnI enzymes were used to perform RFLPs. The results indicated that the phytoplasma associated with the PPT disease belongs to the aster yellows (16Sr1) group and the I-B subgroup. The same phytoplasma was found in all three regions of study. When the sequence of the amplified fragment was compared with the registered Gene Bank (NCBI) sequences (using the BLAST package) a homology of 99% was found with the aster yellows (16Srl) group, "Candidatus Phytoplasma asteris".
author	Gutiérrez-Ibáñez,Ana T Laguna-Cerda,Antonio Rojas-Martínez,Reina González-Garza,Ramiro Salgado-Siclán,Martha L
author_facet	Gutiérrez-Ibáñez,Ana T Laguna-Cerda,Antonio Rojas-Martínez,Reina González-Garza,Ramiro Salgado-Siclán,Martha L
author_sort	Gutiérrez-Ibáñez,Ana T
title	Molecular detection and classification of the phytoplasma that causes purple top in potatoes (Solanum tuberosum) in the State of Mexico
title_short	Molecular detection and classification of the phytoplasma that causes purple top in potatoes (Solanum tuberosum) in the State of Mexico
title_full	Molecular detection and classification of the phytoplasma that causes purple top in potatoes (Solanum tuberosum) in the State of Mexico
title_fullStr	Molecular detection and classification of the phytoplasma that causes purple top in potatoes (Solanum tuberosum) in the State of Mexico
title_full_unstemmed	Molecular detection and classification of the phytoplasma that causes purple top in potatoes (Solanum tuberosum) in the State of Mexico
title_sort	molecular detection and classification of the phytoplasma that causes purple top in potatoes (solanum tuberosum) in the state of mexico
publisher	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000200010
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Molecular detection and classification of the phytoplasma that causes purple top in potatoes (Solanum tuberosum) in the State of Mexico

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