Effect of substrate on the in vitro protein digestibility of extracts generated by Saccharomyces cerevisiae

A. Velásquez, and R. Arias. 2013. Effect of substrate on the in vitro protein digestibility of extracts generated by Saccharomyces cerevisiae. Cien. Inv. Agr. 40(3): 503-511. The in vitro crude protein (CP) digestibility (Divcp), true protein digestibility (Dtp) and degradation rate (kd cp) were mea...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Velásquez,Alejandro, Arias,Rodrigo
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal 2013
Materias:	In vitro protein digestibility protein extract Saccharomyces cerevisiae vegetable substrates
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000300004
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0718-16202013000300004
record_format	dspace
spelling	oai:scielo:S0718-162020130003000042014-09-08Effect of substrate on the in vitro protein digestibility of extracts generated by Saccharomyces cerevisiaeVelásquez,AlejandroArias,Rodrigo In vitro protein digestibility protein extract Saccharomyces cerevisiae vegetable substrates A. Velásquez, and R. Arias. 2013. Effect of substrate on the in vitro protein digestibility of extracts generated by Saccharomyces cerevisiae. Cien. Inv. Agr. 40(3): 503-511. The in vitro crude protein (CP) digestibility (Divcp), true protein digestibility (Dtp) and degradation rate (kd cp) were measured in different protein extracts of Saccharomyces cerevisiae (Sc) yeast. These extracts were generated through a biotechnological method (solid state fermentation). Wheat straw (TWS), barley straw (TBS), chili stubble (TCS), oats hull (TOH) and starch-glucose powder (TSP; control) were used as substrates for fermentation by Sc, and their effect on the aforementioned kinetic parameters in the generated cellular biomass was evaluated. In 12 mL cultivation medium, 2 mL Sc solution inoculum (6.7×10(6) spores mL-1) was added together with 2 g vegetable substrate (dry) and additional nitrogen source, and the mixture was incubated for 7 days at a constant temperature (26°C at pH 5) under aerobic conditions. The average CP content of the yeast biomass (DM) was of 45%. The Divcp and Dtp were analyzed (two phases: pepsin/trypsin-pancreatin) after the extraction of the yeast biomass, and an effect of the fermented substrate on these values (P≤0.001 and P≤0.01, respectively) was observed. The greatest digestibility (Divcp) was obtained with TSP (78.9% CP) and the lowest with TCS (67.1% CP). In addition, differences in the kd cp between the treatments were also observed (P≤0.01). The high contents of CP, Divcp (71.8% on average) and kd cp (16.4% h-1 on average) suggest that Sc yeast biomass has the potential to become an alternative for the production of animal protein supplements.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería ForestalCiencia e investigación agraria v.40 n.3 20132013-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000300004en10.4067/S0718-16202013000300004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	In vitro protein digestibility protein extract Saccharomyces cerevisiae vegetable substrates
spellingShingle	In vitro protein digestibility protein extract Saccharomyces cerevisiae vegetable substrates Velásquez,Alejandro Arias,Rodrigo Effect of substrate on the in vitro protein digestibility of extracts generated by Saccharomyces cerevisiae
description	A. Velásquez, and R. Arias. 2013. Effect of substrate on the in vitro protein digestibility of extracts generated by Saccharomyces cerevisiae. Cien. Inv. Agr. 40(3): 503-511. The in vitro crude protein (CP) digestibility (Divcp), true protein digestibility (Dtp) and degradation rate (kd cp) were measured in different protein extracts of Saccharomyces cerevisiae (Sc) yeast. These extracts were generated through a biotechnological method (solid state fermentation). Wheat straw (TWS), barley straw (TBS), chili stubble (TCS), oats hull (TOH) and starch-glucose powder (TSP; control) were used as substrates for fermentation by Sc, and their effect on the aforementioned kinetic parameters in the generated cellular biomass was evaluated. In 12 mL cultivation medium, 2 mL Sc solution inoculum (6.7×10(6) spores mL-1) was added together with 2 g vegetable substrate (dry) and additional nitrogen source, and the mixture was incubated for 7 days at a constant temperature (26°C at pH 5) under aerobic conditions. The average CP content of the yeast biomass (DM) was of 45%. The Divcp and Dtp were analyzed (two phases: pepsin/trypsin-pancreatin) after the extraction of the yeast biomass, and an effect of the fermented substrate on these values (P≤0.001 and P≤0.01, respectively) was observed. The greatest digestibility (Divcp) was obtained with TSP (78.9% CP) and the lowest with TCS (67.1% CP). In addition, differences in the kd cp between the treatments were also observed (P≤0.01). The high contents of CP, Divcp (71.8% on average) and kd cp (16.4% h-1 on average) suggest that Sc yeast biomass has the potential to become an alternative for the production of animal protein supplements.
author	Velásquez,Alejandro Arias,Rodrigo
author_facet	Velásquez,Alejandro Arias,Rodrigo
author_sort	Velásquez,Alejandro
title	Effect of substrate on the in vitro protein digestibility of extracts generated by Saccharomyces cerevisiae
title_short	Effect of substrate on the in vitro protein digestibility of extracts generated by Saccharomyces cerevisiae
title_full	Effect of substrate on the in vitro protein digestibility of extracts generated by Saccharomyces cerevisiae
title_fullStr	Effect of substrate on the in vitro protein digestibility of extracts generated by Saccharomyces cerevisiae
title_full_unstemmed	Effect of substrate on the in vitro protein digestibility of extracts generated by Saccharomyces cerevisiae
title_sort	effect of substrate on the in vitro protein digestibility of extracts generated by saccharomyces cerevisiae
publisher	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000300004
work_keys_str_mv	AT velasquezalejandro effectofsubstrateontheinvitroproteindigestibilityofextractsgeneratedbysaccharomycescerevisiae AT ariasrodrigo effectofsubstrateontheinvitroproteindigestibilityofextractsgeneratedbysaccharomycescerevisiae
_version_	1714202151612317696

Effect of substrate on the in vitro protein digestibility of extracts generated by Saccharomyces cerevisiae

Ejemplares similares