In vitro culture of Luma chequen from vegetative buds

H. Mancilla, K. Quiroz, A. Arencibia, B. Carrasco, and R. García-Gonzales. 2013. In vitro culture of Luma chequen from vegetative buds. Cien. Inv. Agr. 40(3): 609-615. Luma chequen, a small tree or large shrub belonging to the Myrtaceae family, is endemic to South America and has medicinal, nutritio...

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Autores principales: Mancilla,Héctor, Quiroz,Karla, Arencibia,Ariel, Carrasco,Basilio, García-Gonzales,Rolando
Lenguaje:English
Publicado: Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal 2013
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000300013
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spelling oai:scielo:S0718-162020130003000132014-09-08In vitro culture of Luma chequen from vegetative budsMancilla,HéctorQuiroz,KarlaArencibia,ArielCarrasco,BasilioGarcía-Gonzales,Rolando Aseptic nodal segments in vitro culture Myrtaceae family organogenesis H. Mancilla, K. Quiroz, A. Arencibia, B. Carrasco, and R. García-Gonzales. 2013. In vitro culture of Luma chequen from vegetative buds. Cien. Inv. Agr. 40(3): 609-615. Luma chequen, a small tree or large shrub belonging to the Myrtaceae family, is endemic to South America and has medicinal, nutritional and ornamental potential. However, its native habitat is deteriorating gradually, and it is suffering from the effects of fragmentation that is being caused by the conversion of forest land to agricultural land and the natural expansion of monocultural plantations of exotic species, such as Pinus radiata. The purpose of this work is to develop an effective procedure for establishing in vitro cultures of the native Chilean species L. chequen. Aseptic nodal segments were evaluated after exposure to a disinfecting agent (1% solution of sodium hypochlorite) for different lengths of time. Murashige and Skoog (MS) or Woody Plant (WPM) culture media with 6-Bencilaminopurine (BAP) or 2-isopentenil adenine (2-iP) added to a concentration of 1 mg L-1 were evaluated. Although no significant differences were observed between cultures with and without additives, 40.43% of the explant cultures were successfully established. Furthermore, the choice of basal medium or the addition of plant growth regulators was not found to affect the shoot formation efficiency.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería ForestalCiencia e investigación agraria v.40 n.3 20132013-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000300013en10.4067/S0718-16202013000300013
institution Scielo Chile
collection Scielo Chile
language English
topic Aseptic nodal segments
in vitro culture
Myrtaceae family
organogenesis
spellingShingle Aseptic nodal segments
in vitro culture
Myrtaceae family
organogenesis
Mancilla,Héctor
Quiroz,Karla
Arencibia,Ariel
Carrasco,Basilio
García-Gonzales,Rolando
In vitro culture of Luma chequen from vegetative buds
description H. Mancilla, K. Quiroz, A. Arencibia, B. Carrasco, and R. García-Gonzales. 2013. In vitro culture of Luma chequen from vegetative buds. Cien. Inv. Agr. 40(3): 609-615. Luma chequen, a small tree or large shrub belonging to the Myrtaceae family, is endemic to South America and has medicinal, nutritional and ornamental potential. However, its native habitat is deteriorating gradually, and it is suffering from the effects of fragmentation that is being caused by the conversion of forest land to agricultural land and the natural expansion of monocultural plantations of exotic species, such as Pinus radiata. The purpose of this work is to develop an effective procedure for establishing in vitro cultures of the native Chilean species L. chequen. Aseptic nodal segments were evaluated after exposure to a disinfecting agent (1% solution of sodium hypochlorite) for different lengths of time. Murashige and Skoog (MS) or Woody Plant (WPM) culture media with 6-Bencilaminopurine (BAP) or 2-isopentenil adenine (2-iP) added to a concentration of 1 mg L-1 were evaluated. Although no significant differences were observed between cultures with and without additives, 40.43% of the explant cultures were successfully established. Furthermore, the choice of basal medium or the addition of plant growth regulators was not found to affect the shoot formation efficiency.
author Mancilla,Héctor
Quiroz,Karla
Arencibia,Ariel
Carrasco,Basilio
García-Gonzales,Rolando
author_facet Mancilla,Héctor
Quiroz,Karla
Arencibia,Ariel
Carrasco,Basilio
García-Gonzales,Rolando
author_sort Mancilla,Héctor
title In vitro culture of Luma chequen from vegetative buds
title_short In vitro culture of Luma chequen from vegetative buds
title_full In vitro culture of Luma chequen from vegetative buds
title_fullStr In vitro culture of Luma chequen from vegetative buds
title_full_unstemmed In vitro culture of Luma chequen from vegetative buds
title_sort in vitro culture of luma chequen from vegetative buds
publisher Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
publishDate 2013
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000300013
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