Simultaneous detection of CTV, CEVd and HSVd using Arizona 861 S1 Citron and RT-PCR

R. Camps, M. Castro, and X. Besoain. 2014. Simultaneous detection of CTV, CEVd and HSVd using Arizona 861 S1 Citron and RT-PCR. Cien. Inv. Agr. 41(2): 255-261. Citrus tristeza virus (CTV), Citrus exocortis viroid (CEVd) and Hop stunt viroid (HSVd) are pathogens that affect citrus trees in Chile. The...

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Autores principales:	Camps,Rocío, Castro,Mónica, Besoain,Ximena
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal 2014
Materias:	CEVd citrus CTV diagnostic methods HSVd rapid detection
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202014000200012
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spelling	oai:scielo:S0718-162020140002000122014-09-24Simultaneous detection of CTV, CEVd and HSVd using Arizona 861 S1 Citron and RT-PCRCamps,RocíoCastro,MónicaBesoain,Ximena CEVd citrus CTV diagnostic methods HSVd rapid detection R. Camps, M. Castro, and X. Besoain. 2014. Simultaneous detection of CTV, CEVd and HSVd using Arizona 861 S1 Citron and RT-PCR. Cien. Inv. Agr. 41(2): 255-261. Citrus tristeza virus (CTV), Citrus exocortis viroid (CEVd) and Hop stunt viroid (HSVd) are pathogens that affect citrus trees in Chile. The simultaneous use of a rapid diagnostic method with the effective cleaning of infected budwood is a powerful preventative method for controlling viral diseases. The objective of this study was the multiple detection of CTV, CEVd, and HSVd, through bio-amplification in Arizona 861-S1 citron plants, combined with two incubation temperatures and multiple RT-PCR in two steps. Citron indicator plants were inoculated with the three pathogens, both individually and simultaneously, with four un-inoculated plants as controls. After four months of incubation at the two different temperatures, both the multiple and individually inoculated plants showed symptoms associated with these pathogens, which were subsequently corroborated through the use of multiple RT-PCR in two steps. This diagnostic method could be employed as an efficient, cost-effective alternative to the methods that are currently in use for detecting CTV, CEVd and HSVd, nevertheless its effectiveness should also be evaluated with tests using different isolates of these pathogens.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería ForestalCiencia e investigación agraria v.41 n.2 20142014-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202014000200012en10.4067/S0718-16202014000200012
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	CEVd citrus CTV diagnostic methods HSVd rapid detection
spellingShingle	CEVd citrus CTV diagnostic methods HSVd rapid detection Camps,Rocío Castro,Mónica Besoain,Ximena Simultaneous detection of CTV, CEVd and HSVd using Arizona 861 S1 Citron and RT-PCR
description	R. Camps, M. Castro, and X. Besoain. 2014. Simultaneous detection of CTV, CEVd and HSVd using Arizona 861 S1 Citron and RT-PCR. Cien. Inv. Agr. 41(2): 255-261. Citrus tristeza virus (CTV), Citrus exocortis viroid (CEVd) and Hop stunt viroid (HSVd) are pathogens that affect citrus trees in Chile. The simultaneous use of a rapid diagnostic method with the effective cleaning of infected budwood is a powerful preventative method for controlling viral diseases. The objective of this study was the multiple detection of CTV, CEVd, and HSVd, through bio-amplification in Arizona 861-S1 citron plants, combined with two incubation temperatures and multiple RT-PCR in two steps. Citron indicator plants were inoculated with the three pathogens, both individually and simultaneously, with four un-inoculated plants as controls. After four months of incubation at the two different temperatures, both the multiple and individually inoculated plants showed symptoms associated with these pathogens, which were subsequently corroborated through the use of multiple RT-PCR in two steps. This diagnostic method could be employed as an efficient, cost-effective alternative to the methods that are currently in use for detecting CTV, CEVd and HSVd, nevertheless its effectiveness should also be evaluated with tests using different isolates of these pathogens.
author	Camps,Rocío Castro,Mónica Besoain,Ximena
author_facet	Camps,Rocío Castro,Mónica Besoain,Ximena
author_sort	Camps,Rocío
title	Simultaneous detection of CTV, CEVd and HSVd using Arizona 861 S1 Citron and RT-PCR
title_short	Simultaneous detection of CTV, CEVd and HSVd using Arizona 861 S1 Citron and RT-PCR
title_full	Simultaneous detection of CTV, CEVd and HSVd using Arizona 861 S1 Citron and RT-PCR
title_fullStr	Simultaneous detection of CTV, CEVd and HSVd using Arizona 861 S1 Citron and RT-PCR
title_full_unstemmed	Simultaneous detection of CTV, CEVd and HSVd using Arizona 861 S1 Citron and RT-PCR
title_sort	simultaneous detection of ctv, cevd and hsvd using arizona 861 s1 citron and rt-pcr
publisher	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202014000200012
work_keys_str_mv	AT campsrocio simultaneousdetectionofctvcevdandhsvdusingarizona861s1citronandrtpcr AT castromonica simultaneousdetectionofctvcevdandhsvdusingarizona861s1citronandrtpcr AT besoainximena simultaneousdetectionofctvcevdandhsvdusingarizona861s1citronandrtpcr
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Simultaneous detection of CTV, CEVd and HSVd using Arizona 861 S1 Citron and RT-PCR

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