Enhanced secretion of biocontrol enzymes by Trichoderma harzianum mutant strains in the presence of Rhizoctonia solani cell walls

The secretion of enzymes involved in biocontrol from the wild T. harzianum strains Th11 and Th12 and the mutants Th11A80.1, Th11C40.1 and Th12A10.1 was studied after their cultivation in the presence of R. solani cell walls as the sole carbon source. The results showed that endoprotease activity in...

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Autores principales: Polanco,Rubén, Pino,Constanza, Besoain,Ximena, Montealegre,Jaime, Pérez,Luz M
Lenguaje:English
Publicado: Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal 2015
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202015000200010
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spelling oai:scielo:S0718-162020150002000102015-09-16Enhanced secretion of biocontrol enzymes by Trichoderma harzianum mutant strains in the presence of Rhizoctonia solani cell wallsPolanco,RubénPino,ConstanzaBesoain,XimenaMontealegre,JaimePérez,Luz M ß-1,3-glucanases endoproteases ß-1,4-chitinases The secretion of enzymes involved in biocontrol from the wild T. harzianum strains Th11 and Th12 and the mutants Th11A80.1, Th11C40.1 and Th12A10.1 was studied after their cultivation in the presence of R. solani cell walls as the sole carbon source. The results showed that endoprotease activity in the supernatants from Th11A80.1 and Th11C40.1 increased 2.77 and 4.79-fold, respectively, and the P-1,3-glucanase and P-1,4-chitinase activity from Th12A10.1 increased 1.86 and 1.54-fold, respectively, compared with the corresponding parental strains. The role of the three enzymes in the biocontrol of R. solani is discussed.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería ForestalCiencia e investigación agraria v.42 n.2 20152015-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202015000200010en10.4067/S0718-162020150002000010
institution Scielo Chile
collection Scielo Chile
language English
topic ß-1,3-glucanases
endoproteases
ß-1,4-chitinases
spellingShingle ß-1,3-glucanases
endoproteases
ß-1,4-chitinases
Polanco,Rubén
Pino,Constanza
Besoain,Ximena
Montealegre,Jaime
Pérez,Luz M
Enhanced secretion of biocontrol enzymes by Trichoderma harzianum mutant strains in the presence of Rhizoctonia solani cell walls
description The secretion of enzymes involved in biocontrol from the wild T. harzianum strains Th11 and Th12 and the mutants Th11A80.1, Th11C40.1 and Th12A10.1 was studied after their cultivation in the presence of R. solani cell walls as the sole carbon source. The results showed that endoprotease activity in the supernatants from Th11A80.1 and Th11C40.1 increased 2.77 and 4.79-fold, respectively, and the P-1,3-glucanase and P-1,4-chitinase activity from Th12A10.1 increased 1.86 and 1.54-fold, respectively, compared with the corresponding parental strains. The role of the three enzymes in the biocontrol of R. solani is discussed.
author Polanco,Rubén
Pino,Constanza
Besoain,Ximena
Montealegre,Jaime
Pérez,Luz M
author_facet Polanco,Rubén
Pino,Constanza
Besoain,Ximena
Montealegre,Jaime
Pérez,Luz M
author_sort Polanco,Rubén
title Enhanced secretion of biocontrol enzymes by Trichoderma harzianum mutant strains in the presence of Rhizoctonia solani cell walls
title_short Enhanced secretion of biocontrol enzymes by Trichoderma harzianum mutant strains in the presence of Rhizoctonia solani cell walls
title_full Enhanced secretion of biocontrol enzymes by Trichoderma harzianum mutant strains in the presence of Rhizoctonia solani cell walls
title_fullStr Enhanced secretion of biocontrol enzymes by Trichoderma harzianum mutant strains in the presence of Rhizoctonia solani cell walls
title_full_unstemmed Enhanced secretion of biocontrol enzymes by Trichoderma harzianum mutant strains in the presence of Rhizoctonia solani cell walls
title_sort enhanced secretion of biocontrol enzymes by trichoderma harzianum mutant strains in the presence of rhizoctonia solani cell walls
publisher Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
publishDate 2015
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202015000200010
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