Effect of culture medium and incubation on the characteristics of micropropagated agave plants

Agave potatorum Zucc adventitious shoots were obtained in vitro from stem tissues and rooted in different solid media, pH 5.8, containing the following: 1) different concentrations (50, 75, and 100%) of MS (Murashige and Skoog) mineral salts and 2) different quantities of indole-3-butyric acid (IBA)...

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Autores principales:	Enríquez-del Valle,José R, Antonio-Luis,Karla H, Rodríguez-Ortiz,Gerardo, Campos-Ángeles,Gisela V
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal 2016
Materias:	fluorescent lighting incubation environment indole butyric acid mineral salts solar radiation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202016000200009
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spelling	oai:scielo:S0718-162020160002000092016-10-07Effect of culture medium and incubation on the characteristics of micropropagated agave plantsEnríquez-del Valle,José RAntonio-Luis,Karla HRodríguez-Ortiz,GerardoCampos-Ángeles,Gisela V fluorescent lighting incubation environment indole butyric acid mineral salts solar radiation Agave potatorum Zucc adventitious shoots were obtained in vitro from stem tissues and rooted in different solid media, pH 5.8, containing the following: 1) different concentrations (50, 75, and 100%) of MS (Murashige and Skoog) mineral salts and 2) different quantities of indole-3-butyric acid (IBA) (0, 0.5, 1 mg L-1). The shoots in each medium were separated into two groups and incubated for 84 days under different environments, including a laboratory or greenhouse. The experimental design was completely randomized with a 3×3×2 factorial arrangement. Adventitious roots emerged after 10 to 14 days of incubation, depending on the condition of incubation. In both laboratory and greenhouse settings, after 28 days of incubation, 66.6 and 93.6% of shoots subjected to treatment medium with 75% inorganic salts, 0.5 mg L-1 IBA and medium with 50% inorganic salts, 1 mg L-1 IBA, respectively, had formed roots. On day 40, all shoots had roots. After the 84-day rooting stage, the plants grown from the shoots in the medium with 1 mg L-1 IBA were larger in terms of leaf width, stem diameter, and largest leaf base cross-section area, but similar in the number of roots relative to the plants obtained in the media without IBA. Plant size increased with increasing mineral salt concentrations in the medium. Micropropagated plants obtained from the cultures incubated in a greenhouse and laboratory had an 8.9 and 5.4 cm leaf length, 1.8 and 0.8 cm³ leaf volume, 1.8 and 0.5 cm³ stem diameter, and 6.6 and 5.2 roots, respectively.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería ForestalCiencia e investigación agraria v.43 n.2 20162016-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202016000200009en10.4067/S0718-16202016000200009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	fluorescent lighting incubation environment indole butyric acid mineral salts solar radiation
spellingShingle	fluorescent lighting incubation environment indole butyric acid mineral salts solar radiation Enríquez-del Valle,José R Antonio-Luis,Karla H Rodríguez-Ortiz,Gerardo Campos-Ángeles,Gisela V Effect of culture medium and incubation on the characteristics of micropropagated agave plants
description	Agave potatorum Zucc adventitious shoots were obtained in vitro from stem tissues and rooted in different solid media, pH 5.8, containing the following: 1) different concentrations (50, 75, and 100%) of MS (Murashige and Skoog) mineral salts and 2) different quantities of indole-3-butyric acid (IBA) (0, 0.5, 1 mg L-1). The shoots in each medium were separated into two groups and incubated for 84 days under different environments, including a laboratory or greenhouse. The experimental design was completely randomized with a 3×3×2 factorial arrangement. Adventitious roots emerged after 10 to 14 days of incubation, depending on the condition of incubation. In both laboratory and greenhouse settings, after 28 days of incubation, 66.6 and 93.6% of shoots subjected to treatment medium with 75% inorganic salts, 0.5 mg L-1 IBA and medium with 50% inorganic salts, 1 mg L-1 IBA, respectively, had formed roots. On day 40, all shoots had roots. After the 84-day rooting stage, the plants grown from the shoots in the medium with 1 mg L-1 IBA were larger in terms of leaf width, stem diameter, and largest leaf base cross-section area, but similar in the number of roots relative to the plants obtained in the media without IBA. Plant size increased with increasing mineral salt concentrations in the medium. Micropropagated plants obtained from the cultures incubated in a greenhouse and laboratory had an 8.9 and 5.4 cm leaf length, 1.8 and 0.8 cm³ leaf volume, 1.8 and 0.5 cm³ stem diameter, and 6.6 and 5.2 roots, respectively.
author	Enríquez-del Valle,José R Antonio-Luis,Karla H Rodríguez-Ortiz,Gerardo Campos-Ángeles,Gisela V
author_facet	Enríquez-del Valle,José R Antonio-Luis,Karla H Rodríguez-Ortiz,Gerardo Campos-Ángeles,Gisela V
author_sort	Enríquez-del Valle,José R
title	Effect of culture medium and incubation on the characteristics of micropropagated agave plants
title_short	Effect of culture medium and incubation on the characteristics of micropropagated agave plants
title_full	Effect of culture medium and incubation on the characteristics of micropropagated agave plants
title_fullStr	Effect of culture medium and incubation on the characteristics of micropropagated agave plants
title_full_unstemmed	Effect of culture medium and incubation on the characteristics of micropropagated agave plants
title_sort	effect of culture medium and incubation on the characteristics of micropropagated agave plants
publisher	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202016000200009
work_keys_str_mv	AT enriquezdelvallejoser effectofculturemediumandincubationonthecharacteristicsofmicropropagatedagaveplants AT antonioluiskarlah effectofculturemediumandincubationonthecharacteristicsofmicropropagatedagaveplants AT rodriguezortizgerardo effectofculturemediumandincubationonthecharacteristicsofmicropropagatedagaveplants AT camposangelesgiselav effectofculturemediumandincubationonthecharacteristicsofmicropropagatedagaveplants
_version_	1714202171681013760

Effect of culture medium and incubation on the characteristics of micropropagated agave plants

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