Aging gene pathway of microRNAs 156/157 and 172 is altered in juvenile and adult plants from in vitro propagated Prunus sp.

A. Bastías, R. Almada, P. Rojas, J.M. Donoso, P. Hinrichsen, and B. Sagredo. 2016. Aging gene pathway of microRNAs 156/157 and 172 is altered in juvenile and adult plants from in vitro propagated Prunus sp. Cien. Inv. Agr. 43(3):429-441. In vitro culture is a very popular technique to mass propagate...

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Autores principales:	Bastías,Adriana, Almada,Rubén, Rojas,Pamela, Donoso,José Manuel, Hinrichsen,Patricio, Sagredo,Boris
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal 2016
Materias:	development in vitro culture hase change rejuvenation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202016000300009
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spelling	oai:scielo:S0718-162020160003000092017-02-03Aging gene pathway of microRNAs 156/157 and 172 is altered in juvenile and adult plants from in vitro propagated Prunus sp.Bastías,AdrianaAlmada,RubénRojas,PamelaDonoso,José ManuelHinrichsen,PatricioSagredo,Boris development in vitro culture hase change rejuvenation A. Bastías, R. Almada, P. Rojas, J.M. Donoso, P. Hinrichsen, and B. Sagredo. 2016. Aging gene pathway of microRNAs 156/157 and 172 is altered in juvenile and adult plants from in vitro propagated Prunus sp. Cien. Inv. Agr. 43(3):429-441. In vitro culture is a very popular technique to mass propagate valuable plant genotypes, including Prunus sp. cultivars. Plants that undergo tissue culture processes often change their morphology and behavior due to the "rejuvenation" caused by the plant growth regulators included in the medium. To evaluate the effects of rejuvenation by tissue culture in Prunus sp., the expression patterns of the aging gene pathway described in plant models, which include the highly conserved microRNA (miRNA or miR) 156/157 and 172 families and several of their respective target genes, were analyzed in distinct Prunus sp. genotypes at different phases of maturity, including true seedling and tissue culture micropropagated plants. In genotypes from true seedling plants, the expression of miR156 and miR157 was higher in the leaves of juvenile plants (one year old) than in those of adult plants (six year old). The opposite pattern was observed with miRNA172 expression. Our results suggest that the aging gene pathway is relatively conserved in Prunus and likely plays a key role in vegetative phase change. However, Prunus sp. plants that were rejuvenated and propagated by in vitro methods showed more erratic behavior for miR156 and miR157 and their target genes, suggesting that tissue culture alters the normal control of the aging pathway.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería ForestalCiencia e investigación agraria v.43 n.3 20162016-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202016000300009en10.4067/S0718-16202016000300009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	development in vitro culture hase change rejuvenation
spellingShingle	development in vitro culture hase change rejuvenation Bastías,Adriana Almada,Rubén Rojas,Pamela Donoso,José Manuel Hinrichsen,Patricio Sagredo,Boris Aging gene pathway of microRNAs 156/157 and 172 is altered in juvenile and adult plants from in vitro propagated Prunus sp.
description	A. Bastías, R. Almada, P. Rojas, J.M. Donoso, P. Hinrichsen, and B. Sagredo. 2016. Aging gene pathway of microRNAs 156/157 and 172 is altered in juvenile and adult plants from in vitro propagated Prunus sp. Cien. Inv. Agr. 43(3):429-441. In vitro culture is a very popular technique to mass propagate valuable plant genotypes, including Prunus sp. cultivars. Plants that undergo tissue culture processes often change their morphology and behavior due to the "rejuvenation" caused by the plant growth regulators included in the medium. To evaluate the effects of rejuvenation by tissue culture in Prunus sp., the expression patterns of the aging gene pathway described in plant models, which include the highly conserved microRNA (miRNA or miR) 156/157 and 172 families and several of their respective target genes, were analyzed in distinct Prunus sp. genotypes at different phases of maturity, including true seedling and tissue culture micropropagated plants. In genotypes from true seedling plants, the expression of miR156 and miR157 was higher in the leaves of juvenile plants (one year old) than in those of adult plants (six year old). The opposite pattern was observed with miRNA172 expression. Our results suggest that the aging gene pathway is relatively conserved in Prunus and likely plays a key role in vegetative phase change. However, Prunus sp. plants that were rejuvenated and propagated by in vitro methods showed more erratic behavior for miR156 and miR157 and their target genes, suggesting that tissue culture alters the normal control of the aging pathway.
author	Bastías,Adriana Almada,Rubén Rojas,Pamela Donoso,José Manuel Hinrichsen,Patricio Sagredo,Boris
author_facet	Bastías,Adriana Almada,Rubén Rojas,Pamela Donoso,José Manuel Hinrichsen,Patricio Sagredo,Boris
author_sort	Bastías,Adriana
title	Aging gene pathway of microRNAs 156/157 and 172 is altered in juvenile and adult plants from in vitro propagated Prunus sp.
title_short	Aging gene pathway of microRNAs 156/157 and 172 is altered in juvenile and adult plants from in vitro propagated Prunus sp.
title_full	Aging gene pathway of microRNAs 156/157 and 172 is altered in juvenile and adult plants from in vitro propagated Prunus sp.
title_fullStr	Aging gene pathway of microRNAs 156/157 and 172 is altered in juvenile and adult plants from in vitro propagated Prunus sp.
title_full_unstemmed	Aging gene pathway of microRNAs 156/157 and 172 is altered in juvenile and adult plants from in vitro propagated Prunus sp.
title_sort	aging gene pathway of micrornas 156/157 and 172 is altered in juvenile and adult plants from in vitro propagated prunus sp.
publisher	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202016000300009
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Aging gene pathway of microRNAs 156/157 and 172 is altered in juvenile and adult plants from in vitro propagated Prunus sp.

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