Benzylaminopurine and indol-3-acetic acid concentrations in in vitro proliferation of Agave angustifolia adventitious shoots

Abstract Today, a micropropagation method for Agave angustifolia exists, but for the multiplication of propagules, more information is needed on the diverse components of the culture medium, cytokinins and auxins and their effect on organogenetic response. The objective of this study was to evaluate...

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Autores principales:	Ríos-Ramírez,Suzel C., Enríquez-delValle,José R., Rodríguez-Ortiz,Gerardo, Ruíz-Luna,Judith
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal 2017
Materias:	Benzylaminopurine explant growth regulator indol-3-acetic acid organogenesis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202017000300285
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spelling	oai:scielo:S0718-162020170003002852018-02-20Benzylaminopurine and indol-3-acetic acid concentrations in in vitro proliferation of Agave angustifolia adventitious shootsRíos-Ramírez,Suzel C.Enríquez-delValle,José R.Rodríguez-Ortiz,GerardoRuíz-Luna,Judith Benzylaminopurine explant growth regulator indol-3-acetic acid organogenesis Abstract Today, a micropropagation method for Agave angustifolia exists, but for the multiplication of propagules, more information is needed on the diverse components of the culture medium, cytokinins and auxins and their effect on organogenetic response. The objective of this study was to evaluate dosages of benzylaminopurine and indol-3-acetic acid (IAA) in a culture medium and their effect on the formation of new adventitious shoots (organogenesis). The explants used were clusters of shoots on a common base of A. angustifolia stem tissue. Clusters of three to four shoots were established in different variants of a Murashige and Skoog culture medium that had different concentrations of benzylaminopurine (BAP; 0.5, 1, 2, and 4 mg L−1) and IAA) (0.1, 0.3, and 1 mg L−1). After 16 wk, it was found that a higher proliferation of shoots was positively related to the concentration of growth regulators. Explants formed a greater quantity of shoots in a culture medium with an increase in BAP of up to 4 mg L−1 and in IAA of up to 1 mg L−1, and there was a synergistic effect with BAP to induce the formation of the greatest number of shoots.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería ForestalCiencia e investigación agraria v.44 n.3 20172017-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202017000300285en10.7764/rcia.v44i3.1810
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Benzylaminopurine explant growth regulator indol-3-acetic acid organogenesis
spellingShingle	Benzylaminopurine explant growth regulator indol-3-acetic acid organogenesis Ríos-Ramírez,Suzel C. Enríquez-delValle,José R. Rodríguez-Ortiz,Gerardo Ruíz-Luna,Judith Benzylaminopurine and indol-3-acetic acid concentrations in in vitro proliferation of Agave angustifolia adventitious shoots
description	Abstract Today, a micropropagation method for Agave angustifolia exists, but for the multiplication of propagules, more information is needed on the diverse components of the culture medium, cytokinins and auxins and their effect on organogenetic response. The objective of this study was to evaluate dosages of benzylaminopurine and indol-3-acetic acid (IAA) in a culture medium and their effect on the formation of new adventitious shoots (organogenesis). The explants used were clusters of shoots on a common base of A. angustifolia stem tissue. Clusters of three to four shoots were established in different variants of a Murashige and Skoog culture medium that had different concentrations of benzylaminopurine (BAP; 0.5, 1, 2, and 4 mg L−1) and IAA) (0.1, 0.3, and 1 mg L−1). After 16 wk, it was found that a higher proliferation of shoots was positively related to the concentration of growth regulators. Explants formed a greater quantity of shoots in a culture medium with an increase in BAP of up to 4 mg L−1 and in IAA of up to 1 mg L−1, and there was a synergistic effect with BAP to induce the formation of the greatest number of shoots.
author	Ríos-Ramírez,Suzel C. Enríquez-delValle,José R. Rodríguez-Ortiz,Gerardo Ruíz-Luna,Judith
author_facet	Ríos-Ramírez,Suzel C. Enríquez-delValle,José R. Rodríguez-Ortiz,Gerardo Ruíz-Luna,Judith
author_sort	Ríos-Ramírez,Suzel C.
title	Benzylaminopurine and indol-3-acetic acid concentrations in in vitro proliferation of Agave angustifolia adventitious shoots
title_short	Benzylaminopurine and indol-3-acetic acid concentrations in in vitro proliferation of Agave angustifolia adventitious shoots
title_full	Benzylaminopurine and indol-3-acetic acid concentrations in in vitro proliferation of Agave angustifolia adventitious shoots
title_fullStr	Benzylaminopurine and indol-3-acetic acid concentrations in in vitro proliferation of Agave angustifolia adventitious shoots
title_full_unstemmed	Benzylaminopurine and indol-3-acetic acid concentrations in in vitro proliferation of Agave angustifolia adventitious shoots
title_sort	benzylaminopurine and indol-3-acetic acid concentrations in in vitro proliferation of agave angustifolia adventitious shoots
publisher	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202017000300285
work_keys_str_mv	AT riosramirezsuzelc benzylaminopurineandindol3aceticacidconcentrationsininvitroproliferationofagaveangustifoliaadventitiousshoots AT enriquezdelvallejoser benzylaminopurineandindol3aceticacidconcentrationsininvitroproliferationofagaveangustifoliaadventitiousshoots AT rodriguezortizgerardo benzylaminopurineandindol3aceticacidconcentrationsininvitroproliferationofagaveangustifoliaadventitiousshoots AT ruizlunajudith benzylaminopurineandindol3aceticacidconcentrationsininvitroproliferationofagaveangustifoliaadventitiousshoots
_version_	1714202179715203072

Benzylaminopurine and indol-3-acetic acid concentrations in in vitro proliferation of Agave angustifolia adventitious shoots

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