Characterization of phenolic profile alterations in metal-polluted bee pollen via capillary electrophoresis

Abstract Bee pollen is a conglomerate of plant pollens, and its nutritional contents include bioactive compounds with antioxidant/antiradical potentials. These potentials are conditioned by botanical origin. In Chile, the native flora is diverse and promising in terms of bioactive compounds, but man...

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Autores principales:	Mejías,Enrique, Gómez,Carlos J., Gareil,Pierre, Delaunay,Nathalie, Montenegro,Gloria
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal 2018
Materias:	Antioxidant antiradical activity capillary electrophoresis metals phenol
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202018000100051
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spelling	oai:scielo:S0718-162020180001000512018-05-18Characterization of phenolic profile alterations in metal-polluted bee pollen via capillary electrophoresisMejías,EnriqueGómez,Carlos J.Gareil,PierreDelaunay,NathalieMontenegro,Gloria Antioxidant antiradical activity capillary electrophoresis metals phenol Abstract Bee pollen is a conglomerate of plant pollens, and its nutritional contents include bioactive compounds with antioxidant/antiradical potentials. These potentials are conditioned by botanical origin. In Chile, the native flora is diverse and promising in terms of bioactive compounds, but many plants grow in metal-polluted areas. The associated bioaccumulation could negatively affect the antioxidant/antiradical abilities of bee pollen. To assess the relationship between the bioaccumulation of metals and the antioxidant activity of pollen, complete bee pollen was collected near and far from the Llaima Volcano, albeit in ranges that ensured the same botanical origins. Mellisopalynological analysis determined Escallonia rubra pollen was the most abundant native flora in complete bee pollen. Therefore, E. rubra pollen collected near and far from the Llaima Volcano was evaluated for the following: phenolic compounds via colorimetric assays; antioxidant activity via ferric reducing/antioxidant power assays; antiradical activity via 1,1-diphenyl-2-picrylhydrazyl radical assays; and metal contents via inductively coupled plasma optical emission spectrometry. Llaima samples had higher Cu and Fe but lower Mn contents and lower antioxidant and antiradical capacities than did the control samples. These results were supported by subsequent fortification assays in Llaima E. rubra samples. In fortified samples with significantly higher metal contents, antiradical and antioxidant abilities decreased. Moreover, shifts in migration times were found for naringenin, rutine, and caffeic acid after capillary electrophoresis (CE) analysis in fortified samples. In conclusion, the results indicated an inverse correlation between metal contents and antioxidant/antiradical potentials in bee pollen.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería ForestalCiencia e investigación agraria v.45 n.1 20182018-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202018000100051en10.7764/rcia.v45i1.1890
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Antioxidant antiradical activity capillary electrophoresis metals phenol
spellingShingle	Antioxidant antiradical activity capillary electrophoresis metals phenol Mejías,Enrique Gómez,Carlos J. Gareil,Pierre Delaunay,Nathalie Montenegro,Gloria Characterization of phenolic profile alterations in metal-polluted bee pollen via capillary electrophoresis
description	Abstract Bee pollen is a conglomerate of plant pollens, and its nutritional contents include bioactive compounds with antioxidant/antiradical potentials. These potentials are conditioned by botanical origin. In Chile, the native flora is diverse and promising in terms of bioactive compounds, but many plants grow in metal-polluted areas. The associated bioaccumulation could negatively affect the antioxidant/antiradical abilities of bee pollen. To assess the relationship between the bioaccumulation of metals and the antioxidant activity of pollen, complete bee pollen was collected near and far from the Llaima Volcano, albeit in ranges that ensured the same botanical origins. Mellisopalynological analysis determined Escallonia rubra pollen was the most abundant native flora in complete bee pollen. Therefore, E. rubra pollen collected near and far from the Llaima Volcano was evaluated for the following: phenolic compounds via colorimetric assays; antioxidant activity via ferric reducing/antioxidant power assays; antiradical activity via 1,1-diphenyl-2-picrylhydrazyl radical assays; and metal contents via inductively coupled plasma optical emission spectrometry. Llaima samples had higher Cu and Fe but lower Mn contents and lower antioxidant and antiradical capacities than did the control samples. These results were supported by subsequent fortification assays in Llaima E. rubra samples. In fortified samples with significantly higher metal contents, antiradical and antioxidant abilities decreased. Moreover, shifts in migration times were found for naringenin, rutine, and caffeic acid after capillary electrophoresis (CE) analysis in fortified samples. In conclusion, the results indicated an inverse correlation between metal contents and antioxidant/antiradical potentials in bee pollen.
author	Mejías,Enrique Gómez,Carlos J. Gareil,Pierre Delaunay,Nathalie Montenegro,Gloria
author_facet	Mejías,Enrique Gómez,Carlos J. Gareil,Pierre Delaunay,Nathalie Montenegro,Gloria
author_sort	Mejías,Enrique
title	Characterization of phenolic profile alterations in metal-polluted bee pollen via capillary electrophoresis
title_short	Characterization of phenolic profile alterations in metal-polluted bee pollen via capillary electrophoresis
title_full	Characterization of phenolic profile alterations in metal-polluted bee pollen via capillary electrophoresis
title_fullStr	Characterization of phenolic profile alterations in metal-polluted bee pollen via capillary electrophoresis
title_full_unstemmed	Characterization of phenolic profile alterations in metal-polluted bee pollen via capillary electrophoresis
title_sort	characterization of phenolic profile alterations in metal-polluted bee pollen via capillary electrophoresis
publisher	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202018000100051
work_keys_str_mv	AT mejiasenrique characterizationofphenolicprofilealterationsinmetalpollutedbeepollenviacapillaryelectrophoresis AT gomezcarlosj characterizationofphenolicprofilealterationsinmetalpollutedbeepollenviacapillaryelectrophoresis AT gareilpierre characterizationofphenolicprofilealterationsinmetalpollutedbeepollenviacapillaryelectrophoresis AT delaunaynathalie characterizationofphenolicprofilealterationsinmetalpollutedbeepollenviacapillaryelectrophoresis AT montenegrogloria characterizationofphenolicprofilealterationsinmetalpollutedbeepollenviacapillaryelectrophoresis
_version_	1714202181190549504

Characterization of phenolic profile alterations in metal-polluted bee pollen via capillary electrophoresis

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