Diazotrophic bacteria isolated from Brachiaria spp.: genetic and physiological diversity

Abstract Grass from the genus Brachiaria spp. predominates in pastures with low fertile soils. This scenario highlights the importance of the association with microorganisms to foster plant growth, which becomes essential to the successful establishment of this forage in such environments. This stud...

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Autores principales:	Oliveira,João Tiago Correia, Silva,Gilka Talita, Diniz,Williane Patrícia da Silva, Figueredo,Everthon Fernandes, Santos,Isaneli Batista dos, Lima,Danubia Ramos Moreira de, Quecine,Maria Carolina, Kuklinsky-Sobral,Júlia, Freire,Fernando José
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal 2018
Materias:	N-Acyl homoserine lactones plant growth promoting bacteria plant-microbe interactions
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202018000300277
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spelling	oai:scielo:S0718-162020180003002772019-02-04Diazotrophic bacteria isolated from Brachiaria spp.: genetic and physiological diversityOliveira,João Tiago CorreiaSilva,Gilka TalitaDiniz,Williane Patrícia da SilvaFigueredo,Everthon FernandesSantos,Isaneli Batista dosLima,Danubia Ramos Moreira deQuecine,Maria CarolinaKuklinsky-Sobral,JúliaFreire,Fernando José N-Acyl homoserine lactones plant growth promoting bacteria plant-microbe interactions Abstract Grass from the genus Brachiaria spp. predominates in pastures with low fertile soils. This scenario highlights the importance of the association with microorganisms to foster plant growth, which becomes essential to the successful establishment of this forage in such environments. This study aimed to evaluate the genetic variability and identify the mechanisms of plant growth promotion, in vitro, of bacteria associated with Brachiaria decumbens Stapf. and Brachiaria humidicola (Rendle.) Schweickerdt in Pernambuco, Brazil. We evaluated 20 isolates of diazotrophic bacteria obtained from the endophyte or rhizosphere communities. The genetic characteristics were determined via sequencing the 16S rRNA region, which allowed us to identify ten different bacterial genera: Bacillus sp., Burkholderia sp., Enterobacter sp., Klebsiella sp., Microbacterium sp., Pantoea sp., Ralstonia sp., Rhizobium sp., Sinomonas sp., and Sphingomonas sp., with a specificity of the genus Rhizobium sp. to Brachiaria decumbens Stapf.. The phenotypic and functional characteristics revealed that 100% of the bacterial strains produced indol-3-acetic acid (IAA) with the addition of L-tryptophan, and 60% presented IAA production independent of the L-tryptophan pathway. We also detected that 70% of the isolated bacteria possessed the capacity to solubilize phosphorus. The analysis of the enzymatic output revealed that 30% of the bacterial isolates produced cellulase, 60% produced pectate lyase, 15% produced polygalacturonase, and 30% produced amylase. We also detected the production of N-acyl homoserine lactones in 65% of bacterial strains. In summary, our results showed that plants of B. decumbens Stapf. and B. humidicola (Rendle.) Schweickerdt interacted with different bacterial genera capable of promoting plant growth.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería ForestalCiencia e investigación agraria v.45 n.3 20182018-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202018000300277en10.7764/rcia.v45i3.1949
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	N-Acyl homoserine lactones plant growth promoting bacteria plant-microbe interactions
spellingShingle	N-Acyl homoserine lactones plant growth promoting bacteria plant-microbe interactions Oliveira,João Tiago Correia Silva,Gilka Talita Diniz,Williane Patrícia da Silva Figueredo,Everthon Fernandes Santos,Isaneli Batista dos Lima,Danubia Ramos Moreira de Quecine,Maria Carolina Kuklinsky-Sobral,Júlia Freire,Fernando José Diazotrophic bacteria isolated from Brachiaria spp.: genetic and physiological diversity
description	Abstract Grass from the genus Brachiaria spp. predominates in pastures with low fertile soils. This scenario highlights the importance of the association with microorganisms to foster plant growth, which becomes essential to the successful establishment of this forage in such environments. This study aimed to evaluate the genetic variability and identify the mechanisms of plant growth promotion, in vitro, of bacteria associated with Brachiaria decumbens Stapf. and Brachiaria humidicola (Rendle.) Schweickerdt in Pernambuco, Brazil. We evaluated 20 isolates of diazotrophic bacteria obtained from the endophyte or rhizosphere communities. The genetic characteristics were determined via sequencing the 16S rRNA region, which allowed us to identify ten different bacterial genera: Bacillus sp., Burkholderia sp., Enterobacter sp., Klebsiella sp., Microbacterium sp., Pantoea sp., Ralstonia sp., Rhizobium sp., Sinomonas sp., and Sphingomonas sp., with a specificity of the genus Rhizobium sp. to Brachiaria decumbens Stapf.. The phenotypic and functional characteristics revealed that 100% of the bacterial strains produced indol-3-acetic acid (IAA) with the addition of L-tryptophan, and 60% presented IAA production independent of the L-tryptophan pathway. We also detected that 70% of the isolated bacteria possessed the capacity to solubilize phosphorus. The analysis of the enzymatic output revealed that 30% of the bacterial isolates produced cellulase, 60% produced pectate lyase, 15% produced polygalacturonase, and 30% produced amylase. We also detected the production of N-acyl homoserine lactones in 65% of bacterial strains. In summary, our results showed that plants of B. decumbens Stapf. and B. humidicola (Rendle.) Schweickerdt interacted with different bacterial genera capable of promoting plant growth.
author	Oliveira,João Tiago Correia Silva,Gilka Talita Diniz,Williane Patrícia da Silva Figueredo,Everthon Fernandes Santos,Isaneli Batista dos Lima,Danubia Ramos Moreira de Quecine,Maria Carolina Kuklinsky-Sobral,Júlia Freire,Fernando José
author_facet	Oliveira,João Tiago Correia Silva,Gilka Talita Diniz,Williane Patrícia da Silva Figueredo,Everthon Fernandes Santos,Isaneli Batista dos Lima,Danubia Ramos Moreira de Quecine,Maria Carolina Kuklinsky-Sobral,Júlia Freire,Fernando José
author_sort	Oliveira,João Tiago Correia
title	Diazotrophic bacteria isolated from Brachiaria spp.: genetic and physiological diversity
title_short	Diazotrophic bacteria isolated from Brachiaria spp.: genetic and physiological diversity
title_full	Diazotrophic bacteria isolated from Brachiaria spp.: genetic and physiological diversity
title_fullStr	Diazotrophic bacteria isolated from Brachiaria spp.: genetic and physiological diversity
title_full_unstemmed	Diazotrophic bacteria isolated from Brachiaria spp.: genetic and physiological diversity
title_sort	diazotrophic bacteria isolated from brachiaria spp.: genetic and physiological diversity
publisher	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202018000300277
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_version_	1714202184793456640

Diazotrophic bacteria isolated from Brachiaria spp.: genetic and physiological diversity

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