Propagation and bulblet enhancement of Rhodophiala pratensis from seeds germinated in vitro

Abstract Rhodophiala pratensis is an endemic geophyte from Chile with great importance to the national market of cut flowers and potted and garden plants. However, many geophytes have conservation issues and are considered endangered species. Efficient and controlled propagation techniques have been...

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Autores principales:	Salazar,Carolina, Landeros,Felipe, Bustos,Evelyn, Bravo,Paulina, Pérez,Claudia, Becerra,José, Ríos,Darcy, Uribe,Matilde
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal 2019
Materias:	Endemic species germination Rhodophiala pratensis sucrose
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202019000100012
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spelling	oai:scielo:S0718-162020190001000122019-05-30Propagation and bulblet enhancement of Rhodophiala pratensis from seeds germinated in vitroSalazar,CarolinaLanderos,FelipeBustos,EvelynBravo,PaulinaPérez,ClaudiaBecerra,JoséRíos,DarcyUribe,Matilde Endemic species germination Rhodophiala pratensis sucrose Abstract Rhodophiala pratensis is an endemic geophyte from Chile with great importance to the national market of cut flowers and potted and garden plants. However, many geophytes have conservation issues and are considered endangered species. Efficient and controlled propagation techniques have been useful for the commercial production of this species and for its conservation. A micropropagation protocol using seeds was developed to determine an optimum method to increase bulb production rates and plant production. The effect of sucrose levels in the medium on the growth of bulbs was tested in vitro (30, 60 and 90 g L-1). Then, the effects on shoot and bulb induction of the cytokinin BAP at concentrations of 1.0, 2.0 and 4.0 mg L-1 with the auxin NAA at concentrations of 0.1, 1.0 and 2.0 mg L-1 were analyzed. The germination rate after eight weeks increased to 86% with very low contamination (7%). Bulb growth increased when high levels of sucrose were added to the medium; the optimum sucrose concentration was 90 g L-1. Acclimatization was successful, with a plant survival rate of 63% and an average bulb growth of 0.5 cm. This is the first study to demonstrate the possibility of propagating R. pratensis seeds in vitro.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería ForestalCiencia e investigación agraria v.46 n.1 20192019-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202019000100012en10.7764/rcia.v46i1.1847
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Endemic species germination Rhodophiala pratensis sucrose
spellingShingle	Endemic species germination Rhodophiala pratensis sucrose Salazar,Carolina Landeros,Felipe Bustos,Evelyn Bravo,Paulina Pérez,Claudia Becerra,José Ríos,Darcy Uribe,Matilde Propagation and bulblet enhancement of Rhodophiala pratensis from seeds germinated in vitro
description	Abstract Rhodophiala pratensis is an endemic geophyte from Chile with great importance to the national market of cut flowers and potted and garden plants. However, many geophytes have conservation issues and are considered endangered species. Efficient and controlled propagation techniques have been useful for the commercial production of this species and for its conservation. A micropropagation protocol using seeds was developed to determine an optimum method to increase bulb production rates and plant production. The effect of sucrose levels in the medium on the growth of bulbs was tested in vitro (30, 60 and 90 g L-1). Then, the effects on shoot and bulb induction of the cytokinin BAP at concentrations of 1.0, 2.0 and 4.0 mg L-1 with the auxin NAA at concentrations of 0.1, 1.0 and 2.0 mg L-1 were analyzed. The germination rate after eight weeks increased to 86% with very low contamination (7%). Bulb growth increased when high levels of sucrose were added to the medium; the optimum sucrose concentration was 90 g L-1. Acclimatization was successful, with a plant survival rate of 63% and an average bulb growth of 0.5 cm. This is the first study to demonstrate the possibility of propagating R. pratensis seeds in vitro.
author	Salazar,Carolina Landeros,Felipe Bustos,Evelyn Bravo,Paulina Pérez,Claudia Becerra,José Ríos,Darcy Uribe,Matilde
author_facet	Salazar,Carolina Landeros,Felipe Bustos,Evelyn Bravo,Paulina Pérez,Claudia Becerra,José Ríos,Darcy Uribe,Matilde
author_sort	Salazar,Carolina
title	Propagation and bulblet enhancement of Rhodophiala pratensis from seeds germinated in vitro
title_short	Propagation and bulblet enhancement of Rhodophiala pratensis from seeds germinated in vitro
title_full	Propagation and bulblet enhancement of Rhodophiala pratensis from seeds germinated in vitro
title_fullStr	Propagation and bulblet enhancement of Rhodophiala pratensis from seeds germinated in vitro
title_full_unstemmed	Propagation and bulblet enhancement of Rhodophiala pratensis from seeds germinated in vitro
title_sort	propagation and bulblet enhancement of rhodophiala pratensis from seeds germinated in vitro
publisher	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202019000100012
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_version_	1714202185408970752

Propagation and bulblet enhancement of Rhodophiala pratensis from seeds germinated in vitro

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