Decrease of flavonol synthase enzymatic activity in Ugni molinae Turcz due to the domestication process

Abstract Flavonoid biosynthesis may be affected by plant domestication, with flavonoid production being reduced in proportion to the degree of domestication. In this context, kaempferol (3,4´,5,7-tetrahydroxyflavone) has been identified in the leaves of wild and cultivated Ugni molinae, a berry ende...

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Autores principales:	Chacón-Fuentes,Manuel, Mutis,Ana, Bardehle,Leonardo, Seguel,Ivette, Urzúa,Alejandro, Quiroz,Andrés
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal 2019
Materias:	Cultivated enzyme activity Kaempferol naringenin wild
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202019000100030
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spelling	oai:scielo:S0718-162020190001000302019-05-30Decrease of flavonol synthase enzymatic activity in Ugni molinae Turcz due to the domestication processChacón-Fuentes,ManuelMutis,AnaBardehle,LeonardoSeguel,IvetteUrzúa,AlejandroQuiroz,Andrés Cultivated enzyme activity Kaempferol naringenin wild Abstract Flavonoid biosynthesis may be affected by plant domestication, with flavonoid production being reduced in proportion to the degree of domestication. In this context, kaempferol (3,4´,5,7-tetrahydroxyflavone) has been identified in the leaves of wild and cultivated Ugni molinae, a berry endemic to Chile. The biosynthetic pathway of kaempferol production begins with naringenin (4´,5,7-trihydroxyflavanone), which is converted to dihydrokaempferol (3,4´,5,7-tetrahydroxyflavanone), catalyzed by flavanone 3ß-hydroxylase (FHT) and then converted to kaempferol by a bifunctional enzyme called flavonol synthase (FLS). Therefore, our study aims to evaluate how FLS activity is affected in murtilla plants that are subjected to the domestication process. Kaempferol was quantified from methanolic extracts of leaf samples collected from both cultivated and wild U. molinae plants using high-performance liquid chromatography, and enzyme extraction was performed to determine FLS activity. The results showed that kaempferol concentration in wild plants from the Soloyo (0.14 μg g-1), Mehuín (0.18 μg g-1) and Queule (0.25 μg g-1) sampling areas was higher than in their cultivated counterparts. Our data are consistent with the FLS activity detected in samples obtained from Manzanal Alto (134.79 pKatal, Soloyo (96.48 pKatal), and Mehuín (119.97 pKatal). These samples also exhibited higher enzymatic activity than their cultivated counterparts. Together, these data suggest that FLS activity is negatively affected by the domestication process.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería ForestalCiencia e investigación agraria v.46 n.1 20192019-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202019000100030en10.7764/rcia.v46i1.1955
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Cultivated enzyme activity Kaempferol naringenin wild
spellingShingle	Cultivated enzyme activity Kaempferol naringenin wild Chacón-Fuentes,Manuel Mutis,Ana Bardehle,Leonardo Seguel,Ivette Urzúa,Alejandro Quiroz,Andrés Decrease of flavonol synthase enzymatic activity in Ugni molinae Turcz due to the domestication process
description	Abstract Flavonoid biosynthesis may be affected by plant domestication, with flavonoid production being reduced in proportion to the degree of domestication. In this context, kaempferol (3,4´,5,7-tetrahydroxyflavone) has been identified in the leaves of wild and cultivated Ugni molinae, a berry endemic to Chile. The biosynthetic pathway of kaempferol production begins with naringenin (4´,5,7-trihydroxyflavanone), which is converted to dihydrokaempferol (3,4´,5,7-tetrahydroxyflavanone), catalyzed by flavanone 3ß-hydroxylase (FHT) and then converted to kaempferol by a bifunctional enzyme called flavonol synthase (FLS). Therefore, our study aims to evaluate how FLS activity is affected in murtilla plants that are subjected to the domestication process. Kaempferol was quantified from methanolic extracts of leaf samples collected from both cultivated and wild U. molinae plants using high-performance liquid chromatography, and enzyme extraction was performed to determine FLS activity. The results showed that kaempferol concentration in wild plants from the Soloyo (0.14 μg g-1), Mehuín (0.18 μg g-1) and Queule (0.25 μg g-1) sampling areas was higher than in their cultivated counterparts. Our data are consistent with the FLS activity detected in samples obtained from Manzanal Alto (134.79 pKatal, Soloyo (96.48 pKatal), and Mehuín (119.97 pKatal). These samples also exhibited higher enzymatic activity than their cultivated counterparts. Together, these data suggest that FLS activity is negatively affected by the domestication process.
author	Chacón-Fuentes,Manuel Mutis,Ana Bardehle,Leonardo Seguel,Ivette Urzúa,Alejandro Quiroz,Andrés
author_facet	Chacón-Fuentes,Manuel Mutis,Ana Bardehle,Leonardo Seguel,Ivette Urzúa,Alejandro Quiroz,Andrés
author_sort	Chacón-Fuentes,Manuel
title	Decrease of flavonol synthase enzymatic activity in Ugni molinae Turcz due to the domestication process
title_short	Decrease of flavonol synthase enzymatic activity in Ugni molinae Turcz due to the domestication process
title_full	Decrease of flavonol synthase enzymatic activity in Ugni molinae Turcz due to the domestication process
title_fullStr	Decrease of flavonol synthase enzymatic activity in Ugni molinae Turcz due to the domestication process
title_full_unstemmed	Decrease of flavonol synthase enzymatic activity in Ugni molinae Turcz due to the domestication process
title_sort	decrease of flavonol synthase enzymatic activity in ugni molinae turcz due to the domestication process
publisher	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202019000100030
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_version_	1714202185708863488

Decrease of flavonol synthase enzymatic activity in Ugni molinae Turcz due to the domestication process

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