New seed collections of North American pitseed goosefoot (Chenopodium berlandieri) and efforts to identify its diploid ancestors through whole-genome sequencing

Abstract Pitseed goosefoot (Chenopodium berlandieri) is an ecologically diverse wild/weedy North American species within the primary gene pool for improving South American quinoa (Chenopodium quinoa). Both taxa are 36-chromosome allotetraploids with subgenomes AA and BB. The A genome is found in a l...

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Autores principales:	Jellen,Eric N., Jarvis,David E., Hunt,Spencer P., Mangelsen,Hayley H., Maughan,Peter J.
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal 2019
Materias:	Chenopodium DNA sequencing genetic resources quinoa
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202019000200187
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spelling	oai:scielo:S0718-162020190002001872019-10-07New seed collections of North American pitseed goosefoot (Chenopodium berlandieri) and efforts to identify its diploid ancestors through whole-genome sequencingJellen,Eric N.Jarvis,David E.Hunt,Spencer P.Mangelsen,Hayley H.Maughan,Peter J. Chenopodium DNA sequencing genetic resources quinoa Abstract Pitseed goosefoot (Chenopodium berlandieri) is an ecologically diverse wild/weedy North American species within the primary gene pool for improving South American quinoa (Chenopodium quinoa). Both taxa are 36-chromosome allotetraploids with subgenomes AA and BB. The A genome is found in a large number of diploids in the Americas, along with one Northeast Asian taxon, and was recently shown to be the maternal ancestor, while the paternal B genome is closely related to several extant Eurasian diploids. Two of our primary objectives were 1) to determine the extent of genetic diversity in the allotetraploid C. berlandieri-quinoa-hircinum complex and 2) to characterize the evolutionary path from polyploidization to domestication in these taxa. In an effort to survey genetic diversity, in 2018, we made seed collections of southern Texas, southern Great Plains, and New England coastal ecotypes of C. berlandieri as well as sympatric diploids. With respect to the second goal, we performed whole-genome sequencing of two Sonoran Desert Chenopodium A-genome diploids in subsection Cellulata and Andean cultivated C. pallidicaule in subsection Leiosperma. When paired reads were aligned to the whole-genome reference of C. quinoa strain ‘QQ74’, the match percentages were 99.31, 99.23, and 98.53 for C. watsonii, C. sonorense, and C. pallidicaule, respectively. These data strongly support C. watsonii as being the most closely related of these three species to the A-genome ancestor of quinoa. Ongoing sequencing efforts with a larger panel of diploids are aimed at identifying the maternal ancestor of C. quinoa and C. berlandieri, if extant.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería ForestalCiencia e investigación agraria v.46 n.2 20192019-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202019000200187en10.7764/rcia.v46i2.2150
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Chenopodium DNA sequencing genetic resources quinoa
spellingShingle	Chenopodium DNA sequencing genetic resources quinoa Jellen,Eric N. Jarvis,David E. Hunt,Spencer P. Mangelsen,Hayley H. Maughan,Peter J. New seed collections of North American pitseed goosefoot (Chenopodium berlandieri) and efforts to identify its diploid ancestors through whole-genome sequencing
description	Abstract Pitseed goosefoot (Chenopodium berlandieri) is an ecologically diverse wild/weedy North American species within the primary gene pool for improving South American quinoa (Chenopodium quinoa). Both taxa are 36-chromosome allotetraploids with subgenomes AA and BB. The A genome is found in a large number of diploids in the Americas, along with one Northeast Asian taxon, and was recently shown to be the maternal ancestor, while the paternal B genome is closely related to several extant Eurasian diploids. Two of our primary objectives were 1) to determine the extent of genetic diversity in the allotetraploid C. berlandieri-quinoa-hircinum complex and 2) to characterize the evolutionary path from polyploidization to domestication in these taxa. In an effort to survey genetic diversity, in 2018, we made seed collections of southern Texas, southern Great Plains, and New England coastal ecotypes of C. berlandieri as well as sympatric diploids. With respect to the second goal, we performed whole-genome sequencing of two Sonoran Desert Chenopodium A-genome diploids in subsection Cellulata and Andean cultivated C. pallidicaule in subsection Leiosperma. When paired reads were aligned to the whole-genome reference of C. quinoa strain ‘QQ74’, the match percentages were 99.31, 99.23, and 98.53 for C. watsonii, C. sonorense, and C. pallidicaule, respectively. These data strongly support C. watsonii as being the most closely related of these three species to the A-genome ancestor of quinoa. Ongoing sequencing efforts with a larger panel of diploids are aimed at identifying the maternal ancestor of C. quinoa and C. berlandieri, if extant.
author	Jellen,Eric N. Jarvis,David E. Hunt,Spencer P. Mangelsen,Hayley H. Maughan,Peter J.
author_facet	Jellen,Eric N. Jarvis,David E. Hunt,Spencer P. Mangelsen,Hayley H. Maughan,Peter J.
author_sort	Jellen,Eric N.
title	New seed collections of North American pitseed goosefoot (Chenopodium berlandieri) and efforts to identify its diploid ancestors through whole-genome sequencing
title_short	New seed collections of North American pitseed goosefoot (Chenopodium berlandieri) and efforts to identify its diploid ancestors through whole-genome sequencing
title_full	New seed collections of North American pitseed goosefoot (Chenopodium berlandieri) and efforts to identify its diploid ancestors through whole-genome sequencing
title_fullStr	New seed collections of North American pitseed goosefoot (Chenopodium berlandieri) and efforts to identify its diploid ancestors through whole-genome sequencing
title_full_unstemmed	New seed collections of North American pitseed goosefoot (Chenopodium berlandieri) and efforts to identify its diploid ancestors through whole-genome sequencing
title_sort	new seed collections of north american pitseed goosefoot (chenopodium berlandieri) and efforts to identify its diploid ancestors through whole-genome sequencing
publisher	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202019000200187
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New seed collections of North American pitseed goosefoot (Chenopodium berlandieri) and efforts to identify its diploid ancestors through whole-genome sequencing

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