Probing transfer of an IncP replicon to natural marine bacteria

An IncP plasmid probe (pUCV2), coding for Cm-r, Km-r and bearing Ap (am) and Tc (am) resistance determinants was constructed by transposition of Tn9 (Cm-r) onto plasmid pLM2 for an efficient selection of potential recipients among natural marine bacteria. Using a Dap- E. coli donor, transmission of...

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Autores principales:	Robeson,James, Skarmeta,Ana María
Lenguaje:	English
Publicado:	Universidad de Valparaíso. Facultad de Ciencias del Mar 2000
Materias:	Horizontal transfer IncP probe marine bacteria
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572000000100001
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spelling	oai:scielo:S0718-195720000001000012009-05-05Probing transfer of an IncP replicon to natural marine bacteriaRobeson,JamesSkarmeta,Ana María Horizontal transfer IncP probe marine bacteria An IncP plasmid probe (pUCV2), coding for Cm-r, Km-r and bearing Ap (am) and Tc (am) resistance determinants was constructed by transposition of Tn9 (Cm-r) onto plasmid pLM2 for an efficient selection of potential recipients among natural marine bacteria. Using a Dap- E. coli donor, transmission of pUCV2 to marine bacteria was tested. pUCV2 is transferred to about 4-8% of natural, marine bacterial cells capable of forming colonies on a low nutrient, marine agar medium. The following bacterial genera, commonly found in the marine environment, could be detected when twenty of the transconjugant colonies obtained were identified: Vibrio, Pseudomonas and Aeromonas.info:eu-repo/semantics/openAccessUniversidad de Valparaíso. Facultad de Ciencias del MarRevista de biología marina y oceanografía v.35 n.2 20002000-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572000000100001en10.4067/S0718-19572000000100001
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Horizontal transfer IncP probe marine bacteria
spellingShingle	Horizontal transfer IncP probe marine bacteria Robeson,James Skarmeta,Ana María Probing transfer of an IncP replicon to natural marine bacteria
description	An IncP plasmid probe (pUCV2), coding for Cm-r, Km-r and bearing Ap (am) and Tc (am) resistance determinants was constructed by transposition of Tn9 (Cm-r) onto plasmid pLM2 for an efficient selection of potential recipients among natural marine bacteria. Using a Dap- E. coli donor, transmission of pUCV2 to marine bacteria was tested. pUCV2 is transferred to about 4-8% of natural, marine bacterial cells capable of forming colonies on a low nutrient, marine agar medium. The following bacterial genera, commonly found in the marine environment, could be detected when twenty of the transconjugant colonies obtained were identified: Vibrio, Pseudomonas and Aeromonas.
author	Robeson,James Skarmeta,Ana María
author_facet	Robeson,James Skarmeta,Ana María
author_sort	Robeson,James
title	Probing transfer of an IncP replicon to natural marine bacteria
title_short	Probing transfer of an IncP replicon to natural marine bacteria
title_full	Probing transfer of an IncP replicon to natural marine bacteria
title_fullStr	Probing transfer of an IncP replicon to natural marine bacteria
title_full_unstemmed	Probing transfer of an IncP replicon to natural marine bacteria
title_sort	probing transfer of an incp replicon to natural marine bacteria
publisher	Universidad de Valparaíso. Facultad de Ciencias del Mar
publishDate	2000
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572000000100001
work_keys_str_mv	AT robesonjames probingtransferofanincpreplicontonaturalmarinebacteria AT skarmetaanamaria probingtransferofanincpreplicontonaturalmarinebacteria
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Probing transfer of an IncP replicon to natural marine bacteria

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