Cytogenetic characterization of the silverside fish Odontesthes regia (Humboldt, 1833) (Teleostei: Atheriniformes: Atherinopsidae) from Iquique, Chile

This paper describes the karyotype of Odontesthes regia by means of Giemsa staining, C-banding, to reveal the distribution of the constitutive heterochromatin, and by Ag-staining and fluorescent in situ hybridization (FISH), to locate ribosomal genes (rDNA). The chromosome diploid modal count in the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Muñoz,Carlos, Nirchio,Mauro, Pérez,Julio E, Ron,Ernesto, Oliveira,Claudio, Ferreira,Irani A
Lenguaje:	English
Publicado:	Universidad de Valparaíso. Facultad de Ciencias del Mar 2006
Materias:	Karyotype NOR C-bands FISH
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572006000100008
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0718-19572006000100008
record_format	dspace
spelling	oai:scielo:S0718-195720060001000082006-11-23Cytogenetic characterization of the silverside fish Odontesthes regia (Humboldt, 1833) (Teleostei: Atheriniformes: Atherinopsidae) from Iquique, ChileMuñoz,CarlosNirchio,MauroPérez,Julio ERon,ErnestoOliveira,ClaudioFerreira,Irani A Karyotype NOR C-bands FISH This paper describes the karyotype of Odontesthes regia by means of Giemsa staining, C-banding, to reveal the distribution of the constitutive heterochromatin, and by Ag-staining and fluorescent in situ hybridization (FISH), to locate ribosomal genes (rDNA). The chromosome diploid modal count in the species was 2n = 48. The karyotype is composed of one submetacentric pair (pair 1), 16 subtelocentric pairs (pairs 2 to 17), and 7 acrocentric pairs (pairs 18 to 24). With the exception of pair 1 it was not possible to classify the homologous chromosomes accurately because differences in chromosome size were too slight between adjacent pairs. The distribution of C-banded heterochromatin allowed for a more accurate matching of the majority of chromosomes of the subtelocentric series. Silver staining of metaphase spreads allowed for the identification of Nucleolus Organizer Regions (Ag-NOR) on pair 1. FISH experiments showed that 18S rDNA sequences were located, as expected, in the same chromosome pair identified as the Ag-NOR-bearing oneinfo:eu-repo/semantics/openAccessUniversidad de Valparaíso. Facultad de Ciencias del MarRevista de biología marina y oceanografía v.41 n.1 20062006-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572006000100008en10.4067/S0718-19572006000100008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Karyotype NOR C-bands FISH
spellingShingle	Karyotype NOR C-bands FISH Muñoz,Carlos Nirchio,Mauro Pérez,Julio E Ron,Ernesto Oliveira,Claudio Ferreira,Irani A Cytogenetic characterization of the silverside fish Odontesthes regia (Humboldt, 1833) (Teleostei: Atheriniformes: Atherinopsidae) from Iquique, Chile
description	This paper describes the karyotype of Odontesthes regia by means of Giemsa staining, C-banding, to reveal the distribution of the constitutive heterochromatin, and by Ag-staining and fluorescent in situ hybridization (FISH), to locate ribosomal genes (rDNA). The chromosome diploid modal count in the species was 2n = 48. The karyotype is composed of one submetacentric pair (pair 1), 16 subtelocentric pairs (pairs 2 to 17), and 7 acrocentric pairs (pairs 18 to 24). With the exception of pair 1 it was not possible to classify the homologous chromosomes accurately because differences in chromosome size were too slight between adjacent pairs. The distribution of C-banded heterochromatin allowed for a more accurate matching of the majority of chromosomes of the subtelocentric series. Silver staining of metaphase spreads allowed for the identification of Nucleolus Organizer Regions (Ag-NOR) on pair 1. FISH experiments showed that 18S rDNA sequences were located, as expected, in the same chromosome pair identified as the Ag-NOR-bearing one
author	Muñoz,Carlos Nirchio,Mauro Pérez,Julio E Ron,Ernesto Oliveira,Claudio Ferreira,Irani A
author_facet	Muñoz,Carlos Nirchio,Mauro Pérez,Julio E Ron,Ernesto Oliveira,Claudio Ferreira,Irani A
author_sort	Muñoz,Carlos
title	Cytogenetic characterization of the silverside fish Odontesthes regia (Humboldt, 1833) (Teleostei: Atheriniformes: Atherinopsidae) from Iquique, Chile
title_short	Cytogenetic characterization of the silverside fish Odontesthes regia (Humboldt, 1833) (Teleostei: Atheriniformes: Atherinopsidae) from Iquique, Chile
title_full	Cytogenetic characterization of the silverside fish Odontesthes regia (Humboldt, 1833) (Teleostei: Atheriniformes: Atherinopsidae) from Iquique, Chile
title_fullStr	Cytogenetic characterization of the silverside fish Odontesthes regia (Humboldt, 1833) (Teleostei: Atheriniformes: Atherinopsidae) from Iquique, Chile
title_full_unstemmed	Cytogenetic characterization of the silverside fish Odontesthes regia (Humboldt, 1833) (Teleostei: Atheriniformes: Atherinopsidae) from Iquique, Chile
title_sort	cytogenetic characterization of the silverside fish odontesthes regia (humboldt, 1833) (teleostei: atheriniformes: atherinopsidae) from iquique, chile
publisher	Universidad de Valparaíso. Facultad de Ciencias del Mar
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572006000100008
work_keys_str_mv	AT munozcarlos cytogeneticcharacterizationofthesilversidefishodontesthesregiahumboldt1833teleosteiatheriniformesatherinopsidaefromiquiquechile AT nirchiomauro cytogeneticcharacterizationofthesilversidefishodontesthesregiahumboldt1833teleosteiatheriniformesatherinopsidaefromiquiquechile AT perezjulioe cytogeneticcharacterizationofthesilversidefishodontesthesregiahumboldt1833teleosteiatheriniformesatherinopsidaefromiquiquechile AT ronernesto cytogeneticcharacterizationofthesilversidefishodontesthesregiahumboldt1833teleosteiatheriniformesatherinopsidaefromiquiquechile AT oliveiraclaudio cytogeneticcharacterizationofthesilversidefishodontesthesregiahumboldt1833teleosteiatheriniformesatherinopsidaefromiquiquechile AT ferreirairania cytogeneticcharacterizationofthesilversidefishodontesthesregiahumboldt1833teleosteiatheriniformesatherinopsidaefromiquiquechile
_version_	1714202257130520576

Cytogenetic characterization of the silverside fish Odontesthes regia (Humboldt, 1833) (Teleostei: Atheriniformes: Atherinopsidae) from Iquique, Chile

Ejemplares similares