Enterovirus en aguas naturales de Valparaíso: una propuesta metodológica para su análisis

In Chile, water sanitary quality is determined by the concentration of fecal indicator bacteria. However, numerous investigations have shown that achieving minimum fecal coliform standards do not predict viral contamination, such as enteric viruses. Enteroviruses are microorganisms causing several i...

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Autores principales: Soler,Maritza, Lobos,Soledad, Lorca,Marcos, Navarrete,Ema
Lenguaje:Spanish / Castilian
Publicado: Universidad de Valparaíso. Facultad de Ciencias del Mar 2009
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572009000200023
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spelling oai:scielo:S0718-195720090002000232010-04-08Enterovirus en aguas naturales de Valparaíso: una propuesta metodológica para su análisisSoler,MaritzaLobos,SoledadLorca,MarcosNavarrete,Ema Aquatic pollution cell culture RT-PCR public health virus infections In Chile, water sanitary quality is determined by the concentration of fecal indicator bacteria. However, numerous investigations have shown that achieving minimum fecal coliform standards do not predict viral contamination, such as enteric viruses. Enteroviruses are microorganisms causing several infections and massive outbreaks of illness which represent a serious risk for public health. In the present study, performed during 2001-2003 in Valparaiso, central Chile, natural water samples were collected from five streams and processed for enterovirus detection by tissue culture and reverse transcription (RT) associated to polymerase chain reaction (PCR). None enterovirus was found in any of the natural stream waters. Simulation of an infection, in drinking water and water from one stream was used to check RT-PCR detection of enterovirus.info:eu-repo/semantics/openAccessUniversidad de Valparaíso. Facultad de Ciencias del MarRevista de biología marina y oceanografía v.44 n.2 20092009-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572009000200023es10.4067/S0718-19572009000200023
institution Scielo Chile
collection Scielo Chile
language Spanish / Castilian
topic Aquatic pollution
cell culture
RT-PCR
public health
virus infections
spellingShingle Aquatic pollution
cell culture
RT-PCR
public health
virus infections
Soler,Maritza
Lobos,Soledad
Lorca,Marcos
Navarrete,Ema
Enterovirus en aguas naturales de Valparaíso: una propuesta metodológica para su análisis
description In Chile, water sanitary quality is determined by the concentration of fecal indicator bacteria. However, numerous investigations have shown that achieving minimum fecal coliform standards do not predict viral contamination, such as enteric viruses. Enteroviruses are microorganisms causing several infections and massive outbreaks of illness which represent a serious risk for public health. In the present study, performed during 2001-2003 in Valparaiso, central Chile, natural water samples were collected from five streams and processed for enterovirus detection by tissue culture and reverse transcription (RT) associated to polymerase chain reaction (PCR). None enterovirus was found in any of the natural stream waters. Simulation of an infection, in drinking water and water from one stream was used to check RT-PCR detection of enterovirus.
author Soler,Maritza
Lobos,Soledad
Lorca,Marcos
Navarrete,Ema
author_facet Soler,Maritza
Lobos,Soledad
Lorca,Marcos
Navarrete,Ema
author_sort Soler,Maritza
title Enterovirus en aguas naturales de Valparaíso: una propuesta metodológica para su análisis
title_short Enterovirus en aguas naturales de Valparaíso: una propuesta metodológica para su análisis
title_full Enterovirus en aguas naturales de Valparaíso: una propuesta metodológica para su análisis
title_fullStr Enterovirus en aguas naturales de Valparaíso: una propuesta metodológica para su análisis
title_full_unstemmed Enterovirus en aguas naturales de Valparaíso: una propuesta metodológica para su análisis
title_sort enterovirus en aguas naturales de valparaíso: una propuesta metodológica para su análisis
publisher Universidad de Valparaíso. Facultad de Ciencias del Mar
publishDate 2009
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572009000200023
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AT lobossoledad enterovirusenaguasnaturalesdevalparaisounapropuestametodologicaparasuanalisis
AT lorcamarcos enterovirusenaguasnaturalesdevalparaisounapropuestametodologicaparasuanalisis
AT navarreteema enterovirusenaguasnaturalesdevalparaisounapropuestametodologicaparasuanalisis
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