DNA barcoding of marine polychaetes species of southern Patagonian fjords

Accurate species identification remains a basic first step in any study of biodiversity, particularly for global changes and their consequences. Thus, there is a pressing need for taxonomic expertise in a broad range of taxa. DNA barcoding has proved to be a powerful alternative method to traditiona...

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Autores principales:	Maturana,Claudia S, Moreno,Rodrigo A, Labra,Fabio A, González-Wevar,Claudio A, Rozbaczylo,Nicolás, Carrasco,Franklin D, Poulin,Elie
Lenguaje:	English
Publicado:	Universidad de Valparaíso. Facultad de Ciencias del Mar 2011
Materias:	Molecular taxonomy Polychaeta Cytochrome c Oxydase Subunit I CIMAR-13 Fjords Chile
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572011000100005
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spelling	oai:scielo:S0718-195720110001000052011-06-09DNA barcoding of marine polychaetes species of southern Patagonian fjordsMaturana,Claudia SMoreno,Rodrigo ALabra,Fabio AGonzález-Wevar,Claudio ARozbaczylo,NicolásCarrasco,Franklin DPoulin,Elie Molecular taxonomy Polychaeta Cytochrome c Oxydase Subunit I CIMAR-13 Fjords Chile Accurate species identification remains a basic first step in any study of biodiversity, particularly for global changes and their consequences. Thus, there is a pressing need for taxonomic expertise in a broad range of taxa. DNA barcoding has proved to be a powerful alternative method to traditional morphological approaches, allowing to complement identification techniques for living organisms. In this study, we assess intraspecific and interspecific genetic divergence among marine polychaetes from Patagonian fjords of southern Chile, using mitochondrial Cytochrome c Oxidase Subunit I (COI) gene. Our results showed that a total of 13 polychaetes species identified in this study exhibited high levels of interspecific variation among 31 analyzed sequences. Mean pairwise sequence distances comparisons based on K2P within species ranged from 0.2 to 0.4%. In contrast, interspecific comparisons were much higher and ranged between 18 to 47%, with the exception of the congeneric species Asychis chilensis and Asychis amphiglypta that showed high levels of genetic similarities and absence of reciprocal monophyly. This study presents the first information on DNA barcoding for polychaetes species in the southern Chile, and it establishes the effectiveness of DNA barcoding for identification of marine polychaetes species from Patagonian Fjords, thus making it available to a much broader range of scientists.info:eu-repo/semantics/openAccessUniversidad de Valparaíso. Facultad de Ciencias del MarRevista de biología marina y oceanografía v.46 n.1 20112011-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572011000100005en10.4067/S0718-19572011000100005
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Molecular taxonomy Polychaeta Cytochrome c Oxydase Subunit I CIMAR-13 Fjords Chile
spellingShingle	Molecular taxonomy Polychaeta Cytochrome c Oxydase Subunit I CIMAR-13 Fjords Chile Maturana,Claudia S Moreno,Rodrigo A Labra,Fabio A González-Wevar,Claudio A Rozbaczylo,Nicolás Carrasco,Franklin D Poulin,Elie DNA barcoding of marine polychaetes species of southern Patagonian fjords
description	Accurate species identification remains a basic first step in any study of biodiversity, particularly for global changes and their consequences. Thus, there is a pressing need for taxonomic expertise in a broad range of taxa. DNA barcoding has proved to be a powerful alternative method to traditional morphological approaches, allowing to complement identification techniques for living organisms. In this study, we assess intraspecific and interspecific genetic divergence among marine polychaetes from Patagonian fjords of southern Chile, using mitochondrial Cytochrome c Oxidase Subunit I (COI) gene. Our results showed that a total of 13 polychaetes species identified in this study exhibited high levels of interspecific variation among 31 analyzed sequences. Mean pairwise sequence distances comparisons based on K2P within species ranged from 0.2 to 0.4%. In contrast, interspecific comparisons were much higher and ranged between 18 to 47%, with the exception of the congeneric species Asychis chilensis and Asychis amphiglypta that showed high levels of genetic similarities and absence of reciprocal monophyly. This study presents the first information on DNA barcoding for polychaetes species in the southern Chile, and it establishes the effectiveness of DNA barcoding for identification of marine polychaetes species from Patagonian Fjords, thus making it available to a much broader range of scientists.
author	Maturana,Claudia S Moreno,Rodrigo A Labra,Fabio A González-Wevar,Claudio A Rozbaczylo,Nicolás Carrasco,Franklin D Poulin,Elie
author_facet	Maturana,Claudia S Moreno,Rodrigo A Labra,Fabio A González-Wevar,Claudio A Rozbaczylo,Nicolás Carrasco,Franklin D Poulin,Elie
author_sort	Maturana,Claudia S
title	DNA barcoding of marine polychaetes species of southern Patagonian fjords
title_short	DNA barcoding of marine polychaetes species of southern Patagonian fjords
title_full	DNA barcoding of marine polychaetes species of southern Patagonian fjords
title_fullStr	DNA barcoding of marine polychaetes species of southern Patagonian fjords
title_full_unstemmed	DNA barcoding of marine polychaetes species of southern Patagonian fjords
title_sort	dna barcoding of marine polychaetes species of southern patagonian fjords
publisher	Universidad de Valparaíso. Facultad de Ciencias del Mar
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572011000100005
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DNA barcoding of marine polychaetes species of southern Patagonian fjords

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