Antitumor activity of Actinobacteria isolated in marine sediment from Todos Santos Bay, Baja California, Mexico

The bioactivity of culturable Actinobacteria from marine sediment of the Todos Santos Bay, Mexico was studied, and bacteria were identified by 16S rRNA gene sequences. The strains were cultured in 1 L of culture media A1 and tested for the effects of seawater on growth. The organic components of cul...

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Autores principales:	Becerril-Espinosa,Amayaly, Guerra-Rivas,Graciela, Ayala-Sánchez,Nahara, Soria-Mercado,Irma E
Lenguaje:	English
Publicado:	Universidad de Valparaíso. Facultad de Ciencias del Mar 2012
Materias:	Marine bacteria antitumor activity bacterial diversity phylogeny
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572012000200013
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spelling	oai:scielo:S0718-195720120002000132012-11-29Antitumor activity of Actinobacteria isolated in marine sediment from Todos Santos Bay, Baja California, MexicoBecerril-Espinosa,AmayalyGuerra-Rivas,GracielaAyala-Sánchez,NaharaSoria-Mercado,Irma E Marine bacteria antitumor activity bacterial diversity phylogeny The bioactivity of culturable Actinobacteria from marine sediment of the Todos Santos Bay, Mexico was studied, and bacteria were identified by 16S rRNA gene sequences. The strains were cultured in 1 L of culture media A1 and tested for the effects of seawater on growth. The organic components of cultures were extracted with XAD-7 amberlite and eluted with acetone. The extracts were separated by flash chromatography on C-18 silica gel. The crude extracts and the fractions were tested against colorectal cancer cells HCT-116. In total, twenty six strains were cultured and identified, divided into ten operational taxonomic units (OTUs) with 100% sequence identity, representing 3 families in the order Actinomycetales, belonging to the genus Streptomyces (77%), Micromonospora (20%) and Nocardia (3%); six of those OTUs had not been reported before. Nineteen percent of the crude extracts of the Streptomyces genus showed cytotoxic activity in a range of IC50 of 69.0 to ≤ 0.076 mg·-1. The most active fractions contained compounds identified by their molecular weight and fragmentation patterns as monactin and pamamycin derivatives.info:eu-repo/semantics/openAccessUniversidad de Valparaíso. Facultad de Ciencias del MarRevista de biología marina y oceanografía v.47 n.2 20122012-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572012000200013en10.4067/S0718-19572012000200013
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Marine bacteria antitumor activity bacterial diversity phylogeny
spellingShingle	Marine bacteria antitumor activity bacterial diversity phylogeny Becerril-Espinosa,Amayaly Guerra-Rivas,Graciela Ayala-Sánchez,Nahara Soria-Mercado,Irma E Antitumor activity of Actinobacteria isolated in marine sediment from Todos Santos Bay, Baja California, Mexico
description	The bioactivity of culturable Actinobacteria from marine sediment of the Todos Santos Bay, Mexico was studied, and bacteria were identified by 16S rRNA gene sequences. The strains were cultured in 1 L of culture media A1 and tested for the effects of seawater on growth. The organic components of cultures were extracted with XAD-7 amberlite and eluted with acetone. The extracts were separated by flash chromatography on C-18 silica gel. The crude extracts and the fractions were tested against colorectal cancer cells HCT-116. In total, twenty six strains were cultured and identified, divided into ten operational taxonomic units (OTUs) with 100% sequence identity, representing 3 families in the order Actinomycetales, belonging to the genus Streptomyces (77%), Micromonospora (20%) and Nocardia (3%); six of those OTUs had not been reported before. Nineteen percent of the crude extracts of the Streptomyces genus showed cytotoxic activity in a range of IC50 of 69.0 to ≤ 0.076 mg·-1. The most active fractions contained compounds identified by their molecular weight and fragmentation patterns as monactin and pamamycin derivatives.
author	Becerril-Espinosa,Amayaly Guerra-Rivas,Graciela Ayala-Sánchez,Nahara Soria-Mercado,Irma E
author_facet	Becerril-Espinosa,Amayaly Guerra-Rivas,Graciela Ayala-Sánchez,Nahara Soria-Mercado,Irma E
author_sort	Becerril-Espinosa,Amayaly
title	Antitumor activity of Actinobacteria isolated in marine sediment from Todos Santos Bay, Baja California, Mexico
title_short	Antitumor activity of Actinobacteria isolated in marine sediment from Todos Santos Bay, Baja California, Mexico
title_full	Antitumor activity of Actinobacteria isolated in marine sediment from Todos Santos Bay, Baja California, Mexico
title_fullStr	Antitumor activity of Actinobacteria isolated in marine sediment from Todos Santos Bay, Baja California, Mexico
title_full_unstemmed	Antitumor activity of Actinobacteria isolated in marine sediment from Todos Santos Bay, Baja California, Mexico
title_sort	antitumor activity of actinobacteria isolated in marine sediment from todos santos bay, baja california, mexico
publisher	Universidad de Valparaíso. Facultad de Ciencias del Mar
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572012000200013
work_keys_str_mv	AT becerrilespinosaamayaly antitumoractivityofactinobacteriaisolatedinmarinesedimentfromtodossantosbaybajacaliforniamexico AT guerrarivasgraciela antitumoractivityofactinobacteriaisolatedinmarinesedimentfromtodossantosbaybajacaliforniamexico AT ayalasancheznahara antitumoractivityofactinobacteriaisolatedinmarinesedimentfromtodossantosbaybajacaliforniamexico AT soriamercadoirmae antitumoractivityofactinobacteriaisolatedinmarinesedimentfromtodossantosbaybajacaliforniamexico
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Antitumor activity of Actinobacteria isolated in marine sediment from Todos Santos Bay, Baja California, Mexico

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