Diversidad de nematodos marinos de Chile continental y antártico: una evaluación morfológica y molecular

Free-living nematodes are an important component of the marine benthos. In Chile there have been few studies on this group, and the majority has focused on the morphological aspect only. In this study, ribosomal 18S RNA and mitochondrial COI genes were used as genetic markers to complement morpholog...

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Autores principales:	Valderrama-Aravena,Natalia, Pérez-Araneda,Karla, Avaria-Llautureo,Jorge, Hernández,Cristián E, Lee,Matthew, Brante,Antonio
Lenguaje:	Spanish / Castilian
Publicado:	Universidad de Valparaíso. Facultad de Ciencias del Mar 2014
Materias:	Meiofauna COI 18S nematode
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572014000100017
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

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spelling	oai:scielo:S0718-195720140001000172014-08-18Diversidad de nematodos marinos de Chile continental y antártico: una evaluación morfológica y molecularValderrama-Aravena,NataliaPérez-Araneda,KarlaAvaria-Llautureo,JorgeHernández,Cristián ELee,MatthewBrante,Antonio Meiofauna COI 18S nematode Free-living nematodes are an important component of the marine benthos. In Chile there have been few studies on this group, and the majority has focused on the morphological aspect only. In this study, ribosomal 18S RNA and mitochondrial COI genes were used as genetic markers to complement morphological analyses to study the continental and Antarctic Chilean marine nematofauna. Different protocols for fixing, extracting and amplifying DNA were also tested. Not all the possible combinations produced good results. In fact, only the 18S gene showed consistently results. Phylogenetic analyses showed some discordance between classical taxonomy (i.e., based on morphology) and molecular data. These results suggest that taxonomic classification using integrative approaches including morphological characteristics and molecular information is needed to study the diversity and evolution of this complex group.info:eu-repo/semantics/openAccessUniversidad de Valparaíso. Facultad de Ciencias del MarRevista de biología marina y oceanografía v.49 n.1 20142014-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572014000100017es10.4067/S0718-19572014000100017
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Meiofauna COI 18S nematode
spellingShingle	Meiofauna COI 18S nematode Valderrama-Aravena,Natalia Pérez-Araneda,Karla Avaria-Llautureo,Jorge Hernández,Cristián E Lee,Matthew Brante,Antonio Diversidad de nematodos marinos de Chile continental y antártico: una evaluación morfológica y molecular
description	Free-living nematodes are an important component of the marine benthos. In Chile there have been few studies on this group, and the majority has focused on the morphological aspect only. In this study, ribosomal 18S RNA and mitochondrial COI genes were used as genetic markers to complement morphological analyses to study the continental and Antarctic Chilean marine nematofauna. Different protocols for fixing, extracting and amplifying DNA were also tested. Not all the possible combinations produced good results. In fact, only the 18S gene showed consistently results. Phylogenetic analyses showed some discordance between classical taxonomy (i.e., based on morphology) and molecular data. These results suggest that taxonomic classification using integrative approaches including morphological characteristics and molecular information is needed to study the diversity and evolution of this complex group.
author	Valderrama-Aravena,Natalia Pérez-Araneda,Karla Avaria-Llautureo,Jorge Hernández,Cristián E Lee,Matthew Brante,Antonio
author_facet	Valderrama-Aravena,Natalia Pérez-Araneda,Karla Avaria-Llautureo,Jorge Hernández,Cristián E Lee,Matthew Brante,Antonio
author_sort	Valderrama-Aravena,Natalia
title	Diversidad de nematodos marinos de Chile continental y antártico: una evaluación morfológica y molecular
title_short	Diversidad de nematodos marinos de Chile continental y antártico: una evaluación morfológica y molecular
title_full	Diversidad de nematodos marinos de Chile continental y antártico: una evaluación morfológica y molecular
title_fullStr	Diversidad de nematodos marinos de Chile continental y antártico: una evaluación morfológica y molecular
title_full_unstemmed	Diversidad de nematodos marinos de Chile continental y antártico: una evaluación morfológica y molecular
title_sort	diversidad de nematodos marinos de chile continental y antártico: una evaluación morfológica y molecular
publisher	Universidad de Valparaíso. Facultad de Ciencias del Mar
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572014000100017
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Diversidad de nematodos marinos de Chile continental y antártico: una evaluación morfológica y molecular

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