Crecimiento y supervivencia de larvas D, pedivéligeras y postlarvas de Gari solida (Mollusca: Psammobiidae), provenientes de reproductores acondicionados

Adults of Gari solida were maintained at a room temperature of around 14°C for approximately 90 days, fed with a microalgae mix 300,000 cell ml-1, after which spawning was induced in 20 specimens. The oocytes obtained were fertilized at a 10:1 sperm/oocyte ratio and the embryos were incubated in a s...

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Autores principales:	Contreras-Guzmán,Ramiro A, Puebla-Arce,Claudia E, Pacheco-Sánchez,Elisa G, Contreras-Saldaña,Francisco J
Lenguaje:	Spanish / Castilian
Publicado:	Universidad de Valparaíso. Facultad de Ciencias del Mar 2014
Materias:	Bivalves larval development clams
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572014000300018
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spelling	oai:scielo:S0718-195720140003000182015-01-14Crecimiento y supervivencia de larvas D, pedivéligeras y postlarvas de Gari solida (Mollusca: Psammobiidae), provenientes de reproductores acondicionadosContreras-Guzmán,Ramiro APuebla-Arce,Claudia EPacheco-Sánchez,Elisa GContreras-Saldaña,Francisco J Bivalves larval development clams Adults of Gari solida were maintained at a room temperature of around 14°C for approximately 90 days, fed with a microalgae mix 300,000 cell ml-1, after which spawning was induced in 20 specimens. The oocytes obtained were fertilized at a 10:1 sperm/oocyte ratio and the embryos were incubated in a specially designed system (`hatching system') for a period of 96 h, until D-shape larvae without gelatinous coating were obtained. Larval culture was undertaken in cylindrical 200 L tanks at 13 ± 1.3°C, with seawater filtered to 1 µm and UV irradiation, at an initial density of 9.7 larvae ml-1, with water renewal every 2-3 days; D-shape larvae were fed with Isochrysis aff. galbana and throughout the umbonate, pediveliger and postlarval stages, with a mix of Isochrysis aff. galbana and Chaetoceros calcitrans (7:3), where concentration was proportioned in accordance with culture density. The umbonate larval stage was detected at day 11 of culture and the pediveliger stage, at day 22, starting from the free-swimming D-shape larvae. Metamorphosis was induced with an epinephrine bath at 0.005 N concentration and postlarvae obtained at day 26 of culture were subsequently cultured up to day 35. Average sizes reached were 122 ± 13 µm, 158 ± 12 µm, 185 ± 5 µm and 216 ± 24 µm for the `D', umbonate, pediveliger and postlarval stages, respectively. Survival up to postlarvae stage was 0.005%. Development of G. solida larvae and postlarvae culture is in its initial phases and improvements in all stages are necessary, from breeder conditioning onwards.info:eu-repo/semantics/openAccessUniversidad de Valparaíso. Facultad de Ciencias del MarRevista de biología marina y oceanografía v.49 n.3 20142014-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572014000300018es10.4067/S0718-19572014000300018
institution	Scielo Chile
collection	Scielo Chile
language	Spanish / Castilian
topic	Bivalves larval development clams
spellingShingle	Bivalves larval development clams Contreras-Guzmán,Ramiro A Puebla-Arce,Claudia E Pacheco-Sánchez,Elisa G Contreras-Saldaña,Francisco J Crecimiento y supervivencia de larvas D, pedivéligeras y postlarvas de Gari solida (Mollusca: Psammobiidae), provenientes de reproductores acondicionados
description	Adults of Gari solida were maintained at a room temperature of around 14°C for approximately 90 days, fed with a microalgae mix 300,000 cell ml-1, after which spawning was induced in 20 specimens. The oocytes obtained were fertilized at a 10:1 sperm/oocyte ratio and the embryos were incubated in a specially designed system (`hatching system') for a period of 96 h, until D-shape larvae without gelatinous coating were obtained. Larval culture was undertaken in cylindrical 200 L tanks at 13 ± 1.3°C, with seawater filtered to 1 µm and UV irradiation, at an initial density of 9.7 larvae ml-1, with water renewal every 2-3 days; D-shape larvae were fed with Isochrysis aff. galbana and throughout the umbonate, pediveliger and postlarval stages, with a mix of Isochrysis aff. galbana and Chaetoceros calcitrans (7:3), where concentration was proportioned in accordance with culture density. The umbonate larval stage was detected at day 11 of culture and the pediveliger stage, at day 22, starting from the free-swimming D-shape larvae. Metamorphosis was induced with an epinephrine bath at 0.005 N concentration and postlarvae obtained at day 26 of culture were subsequently cultured up to day 35. Average sizes reached were 122 ± 13 µm, 158 ± 12 µm, 185 ± 5 µm and 216 ± 24 µm for the `D', umbonate, pediveliger and postlarval stages, respectively. Survival up to postlarvae stage was 0.005%. Development of G. solida larvae and postlarvae culture is in its initial phases and improvements in all stages are necessary, from breeder conditioning onwards.
author	Contreras-Guzmán,Ramiro A Puebla-Arce,Claudia E Pacheco-Sánchez,Elisa G Contreras-Saldaña,Francisco J
author_facet	Contreras-Guzmán,Ramiro A Puebla-Arce,Claudia E Pacheco-Sánchez,Elisa G Contreras-Saldaña,Francisco J
author_sort	Contreras-Guzmán,Ramiro A
title	Crecimiento y supervivencia de larvas D, pedivéligeras y postlarvas de Gari solida (Mollusca: Psammobiidae), provenientes de reproductores acondicionados
title_short	Crecimiento y supervivencia de larvas D, pedivéligeras y postlarvas de Gari solida (Mollusca: Psammobiidae), provenientes de reproductores acondicionados
title_full	Crecimiento y supervivencia de larvas D, pedivéligeras y postlarvas de Gari solida (Mollusca: Psammobiidae), provenientes de reproductores acondicionados
title_fullStr	Crecimiento y supervivencia de larvas D, pedivéligeras y postlarvas de Gari solida (Mollusca: Psammobiidae), provenientes de reproductores acondicionados
title_full_unstemmed	Crecimiento y supervivencia de larvas D, pedivéligeras y postlarvas de Gari solida (Mollusca: Psammobiidae), provenientes de reproductores acondicionados
title_sort	crecimiento y supervivencia de larvas d, pedivéligeras y postlarvas de gari solida (mollusca: psammobiidae), provenientes de reproductores acondicionados
publisher	Universidad de Valparaíso. Facultad de Ciencias del Mar
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572014000300018
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Crecimiento y supervivencia de larvas D, pedivéligeras y postlarvas de Gari solida (Mollusca: Psammobiidae), provenientes de reproductores acondicionados

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