Structure of denitrifying communities reducing N2O at suboxic waters off northern Chile and Perú
The nosZ gene, which encodes for N2O reduction to N2, was used to study the structure of denitrifying communities in the oxygen minimum zone off Chilean and Peruvian coast throughout terminal restriction fragment length polymorphism (TRFLP) and cloning of nosZ genes. TRFLP analysis showed little div...
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| Autores principales: | , , |
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| Lenguaje: | English |
| Publicado: |
Universidad de Valparaíso. Facultad de Ciencias del Mar
2015
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| Materias: | |
| Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572015000100008 |
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| Sumario: | The nosZ gene, which encodes for N2O reduction to N2, was used to study the structure of denitrifying communities in the oxygen minimum zone off Chilean and Peruvian coast throughout terminal restriction fragment length polymorphism (TRFLP) and cloning of nosZ genes. TRFLP analysis showed little diversity of nosZ genes at suboxic depths (Oxygen Minimum Zone´s core) compared with depths where O2 largely varied (upper limit of OMZ or ULOMZ). The nosZ-denitrifying communities showed differences in its structure between geographical locations and time sampling suggesting an association with the shift in the environmental conditions. The canonical correspondence analysis showed that the environmental parameters selected as predictor variables (N2O, O2, NH4+ and NO2-) explained well the differences in nosZ-denitrifying community composition among sampling sites. The phylogenetic analysis showed little nosZ sequence diversity and grouped 81% of nosZ-clones near the cluster of sediments sequences from Pacific. Our sequences did not branch with any known denitrifying bacteria or seawater nosZ-sequences available, demonstrating the novelty of phylotypes founded in this area. |
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