Structure of denitrifying communities reducing N2O at suboxic waters off northern Chile and Perú

The nosZ gene, which encodes for N2O reduction to N2, was used to study the structure of denitrifying communities in the oxygen minimum zone off Chilean and Peruvian coast throughout terminal restriction fragment length polymorphism (TRFLP) and cloning of nosZ genes. TRFLP analysis showed little div...

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Autores principales:	Castro-González,Maribeb, Ulloa,Osvaldo, Farías,Laura
Lenguaje:	English
Publicado:	Universidad de Valparaíso. Facultad de Ciencias del Mar 2015
Materias:	Eastern South Pacific nosZ gene Oxygen Minimum Zone
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572015000100008
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spelling	oai:scielo:S0718-195720150001000082015-08-11Structure of denitrifying communities reducing N2O at suboxic waters off northern Chile and PerúCastro-González,MaribebUlloa,OsvaldoFarías,Laura Eastern South Pacific nosZ gene Oxygen Minimum Zone The nosZ gene, which encodes for N2O reduction to N2, was used to study the structure of denitrifying communities in the oxygen minimum zone off Chilean and Peruvian coast throughout terminal restriction fragment length polymorphism (TRFLP) and cloning of nosZ genes. TRFLP analysis showed little diversity of nosZ genes at suboxic depths (Oxygen Minimum Zone´s core) compared with depths where O2 largely varied (upper limit of OMZ or ULOMZ). The nosZ-denitrifying communities showed differences in its structure between geographical locations and time sampling suggesting an association with the shift in the environmental conditions. The canonical correspondence analysis showed that the environmental parameters selected as predictor variables (N2O, O2, NH4+ and NO2-) explained well the differences in nosZ-denitrifying community composition among sampling sites. The phylogenetic analysis showed little nosZ sequence diversity and grouped 81% of nosZ-clones near the cluster of sediments sequences from Pacific. Our sequences did not branch with any known denitrifying bacteria or seawater nosZ-sequences available, demonstrating the novelty of phylotypes founded in this area.info:eu-repo/semantics/openAccessUniversidad de Valparaíso. Facultad de Ciencias del MarRevista de biología marina y oceanografía v.50 n.1 20152015-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572015000100008en10.4067/S0718-19572015000100008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Eastern South Pacific nosZ gene Oxygen Minimum Zone
spellingShingle	Eastern South Pacific nosZ gene Oxygen Minimum Zone Castro-González,Maribeb Ulloa,Osvaldo Farías,Laura Structure of denitrifying communities reducing N2O at suboxic waters off northern Chile and Perú
description	The nosZ gene, which encodes for N2O reduction to N2, was used to study the structure of denitrifying communities in the oxygen minimum zone off Chilean and Peruvian coast throughout terminal restriction fragment length polymorphism (TRFLP) and cloning of nosZ genes. TRFLP analysis showed little diversity of nosZ genes at suboxic depths (Oxygen Minimum Zone´s core) compared with depths where O2 largely varied (upper limit of OMZ or ULOMZ). The nosZ-denitrifying communities showed differences in its structure between geographical locations and time sampling suggesting an association with the shift in the environmental conditions. The canonical correspondence analysis showed that the environmental parameters selected as predictor variables (N2O, O2, NH4+ and NO2-) explained well the differences in nosZ-denitrifying community composition among sampling sites. The phylogenetic analysis showed little nosZ sequence diversity and grouped 81% of nosZ-clones near the cluster of sediments sequences from Pacific. Our sequences did not branch with any known denitrifying bacteria or seawater nosZ-sequences available, demonstrating the novelty of phylotypes founded in this area.
author	Castro-González,Maribeb Ulloa,Osvaldo Farías,Laura
author_facet	Castro-González,Maribeb Ulloa,Osvaldo Farías,Laura
author_sort	Castro-González,Maribeb
title	Structure of denitrifying communities reducing N2O at suboxic waters off northern Chile and Perú
title_short	Structure of denitrifying communities reducing N2O at suboxic waters off northern Chile and Perú
title_full	Structure of denitrifying communities reducing N2O at suboxic waters off northern Chile and Perú
title_fullStr	Structure of denitrifying communities reducing N2O at suboxic waters off northern Chile and Perú
title_full_unstemmed	Structure of denitrifying communities reducing N2O at suboxic waters off northern Chile and Perú
title_sort	structure of denitrifying communities reducing n2o at suboxic waters off northern chile and perú
publisher	Universidad de Valparaíso. Facultad de Ciencias del Mar
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572015000100008
work_keys_str_mv	AT castrogonzalezmaribeb structureofdenitrifyingcommunitiesreducingn2oatsuboxicwatersoffnorthernchileandperu AT ulloaosvaldo structureofdenitrifyingcommunitiesreducingn2oatsuboxicwatersoffnorthernchileandperu AT fariaslaura structureofdenitrifyingcommunitiesreducingn2oatsuboxicwatersoffnorthernchileandperu
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Structure of denitrifying communities reducing N2O at suboxic waters off northern Chile and Perú

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