Response to oxidative stress induced by high light and carbon dioxide (CO2) in the biodiesel producer model Nannochloropsis salina (Ochrophyta, Eustigmatales)

Due to overconsumption of fossil fuels, microalgae have arrived as an alternative source of biofuel. Looking forward to generate a sustainable process, it is proposed to couple the cultures to CO2 emission sources, reaching in this way higher biomass performance and helping in the way with the captu...

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Autores principales:	Yangüez,Karen, Lovazzano,Carlos, Contreras-Porcia,Loretto, Ehrenfeld,Nicole
Lenguaje:	English
Publicado:	Universidad de Valparaíso. Facultad de Ciencias del Mar 2015
Materias:	Nannochloropsis salina reactive oxygen species antioxidant enzymes carbon dioxide qPCR
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572015000200003
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spelling	oai:scielo:S0718-195720150002000032015-07-24Response to oxidative stress induced by high light and carbon dioxide (CO2) in the biodiesel producer model Nannochloropsis salina (Ochrophyta, Eustigmatales)Yangüez,KarenLovazzano,CarlosContreras-Porcia,LorettoEhrenfeld,Nicole Nannochloropsis salina reactive oxygen species antioxidant enzymes carbon dioxide qPCR Due to overconsumption of fossil fuels, microalgae have arrived as an alternative source of biofuel. Looking forward to generate a sustainable process, it is proposed to couple the cultures to CO2 emission sources, reaching in this way higher biomass performance and helping in the way with the capture of carbon released by the combustion processes. Nannochloropsis salina is a microalgae from the Monodopsidaceae family, which is easy to grow and produces high value compounds like essential pigments, polyunsaturated fatty acids and high amounts of lipids. Previous studies showed that adding CO2 to cultures (until 2%) generated an increment in biomass and in the production of fatty acids. However, these conditions also induce acidification of the media, a condition that may promote the generation of oxygen reactive species. In this work, the antioxidant performance of N. salina was studied under different culture conditions involving CO2 through 3 different approaches: analysis of antioxidant enzymatic activities (catalase, ascorbate peroxidase and peroxiredoxine), analysis of gene expression and the quantification of H2O2, phenolic compounds and lipoperoxides (e.g., cell damage marker). The results obtained suggest that an increase in the CO2 concentration in the cultures (15,000 ppm), together with high light (1,000 µmol m-1 s-1) induces an oxidative stress condition in N. salina cells. However, the antioxidant response observed in the microalgae manages to soften this stress, adapting themselves to these conditions without affecting their global performance.info:eu-repo/semantics/openAccessUniversidad de Valparaíso. Facultad de Ciencias del MarRevista de biología marina y oceanografía v.50 suppl.1 20152015-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572015000200003en10.4067/S0718-19572015000200003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Nannochloropsis salina reactive oxygen species antioxidant enzymes carbon dioxide qPCR
spellingShingle	Nannochloropsis salina reactive oxygen species antioxidant enzymes carbon dioxide qPCR Yangüez,Karen Lovazzano,Carlos Contreras-Porcia,Loretto Ehrenfeld,Nicole Response to oxidative stress induced by high light and carbon dioxide (CO2) in the biodiesel producer model Nannochloropsis salina (Ochrophyta, Eustigmatales)
description	Due to overconsumption of fossil fuels, microalgae have arrived as an alternative source of biofuel. Looking forward to generate a sustainable process, it is proposed to couple the cultures to CO2 emission sources, reaching in this way higher biomass performance and helping in the way with the capture of carbon released by the combustion processes. Nannochloropsis salina is a microalgae from the Monodopsidaceae family, which is easy to grow and produces high value compounds like essential pigments, polyunsaturated fatty acids and high amounts of lipids. Previous studies showed that adding CO2 to cultures (until 2%) generated an increment in biomass and in the production of fatty acids. However, these conditions also induce acidification of the media, a condition that may promote the generation of oxygen reactive species. In this work, the antioxidant performance of N. salina was studied under different culture conditions involving CO2 through 3 different approaches: analysis of antioxidant enzymatic activities (catalase, ascorbate peroxidase and peroxiredoxine), analysis of gene expression and the quantification of H2O2, phenolic compounds and lipoperoxides (e.g., cell damage marker). The results obtained suggest that an increase in the CO2 concentration in the cultures (15,000 ppm), together with high light (1,000 µmol m-1 s-1) induces an oxidative stress condition in N. salina cells. However, the antioxidant response observed in the microalgae manages to soften this stress, adapting themselves to these conditions without affecting their global performance.
author	Yangüez,Karen Lovazzano,Carlos Contreras-Porcia,Loretto Ehrenfeld,Nicole
author_facet	Yangüez,Karen Lovazzano,Carlos Contreras-Porcia,Loretto Ehrenfeld,Nicole
author_sort	Yangüez,Karen
title	Response to oxidative stress induced by high light and carbon dioxide (CO2) in the biodiesel producer model Nannochloropsis salina (Ochrophyta, Eustigmatales)
title_short	Response to oxidative stress induced by high light and carbon dioxide (CO2) in the biodiesel producer model Nannochloropsis salina (Ochrophyta, Eustigmatales)
title_full	Response to oxidative stress induced by high light and carbon dioxide (CO2) in the biodiesel producer model Nannochloropsis salina (Ochrophyta, Eustigmatales)
title_fullStr	Response to oxidative stress induced by high light and carbon dioxide (CO2) in the biodiesel producer model Nannochloropsis salina (Ochrophyta, Eustigmatales)
title_full_unstemmed	Response to oxidative stress induced by high light and carbon dioxide (CO2) in the biodiesel producer model Nannochloropsis salina (Ochrophyta, Eustigmatales)
title_sort	response to oxidative stress induced by high light and carbon dioxide (co2) in the biodiesel producer model nannochloropsis salina (ochrophyta, eustigmatales)
publisher	Universidad de Valparaíso. Facultad de Ciencias del Mar
publishDate	2015
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572015000200003
work_keys_str_mv	AT yanguezkaren responsetooxidativestressinducedbyhighlightandcarbondioxideco2inthebiodieselproducermodelnannochloropsissalinaochrophytaeustigmatales AT lovazzanocarlos responsetooxidativestressinducedbyhighlightandcarbondioxideco2inthebiodieselproducermodelnannochloropsissalinaochrophytaeustigmatales AT contrerasporcialoretto responsetooxidativestressinducedbyhighlightandcarbondioxideco2inthebiodieselproducermodelnannochloropsissalinaochrophytaeustigmatales AT ehrenfeldnicole responsetooxidativestressinducedbyhighlightandcarbondioxideco2inthebiodieselproducermodelnannochloropsissalinaochrophytaeustigmatales
_version_	1714202346593976320

Response to oxidative stress induced by high light and carbon dioxide (CO2) in the biodiesel producer model Nannochloropsis salina (Ochrophyta, Eustigmatales)

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