Decreasing of bacterial content in Isochrysis galbana cultures by using some antibiotics

The axenic microalgae cultures are a difficult task and they are hard to maintain. Microalgae cultures with reduced bacterial load can be an option to axenic microalgae cultures to produce compounds with biotechnological and pharmaceutical potential. Also they can be used for cryopreservation and in...

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Autores principales:	Molina-Cárdenas,Ceres A, Sánchez-Saavedra,M. del Pilar, Licea-Navarro,Alexei Fedorovish
Lenguaje:	English
Publicado:	Universidad de Valparaíso. Facultad de Ciencias del Mar 2016
Materias:	Bacterial load Isochrysis galbana antibiotics centrifugation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572016000100010
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spelling	oai:scielo:S0718-195720160001000102016-06-24Decreasing of bacterial content in Isochrysis galbana cultures by using some antibioticsMolina-Cárdenas,Ceres ASánchez-Saavedra,M. del PilarLicea-Navarro,Alexei Fedorovish Bacterial load Isochrysis galbana antibiotics centrifugation The axenic microalgae cultures are a difficult task and they are hard to maintain. Microalgae cultures with reduced bacterial load can be an option to axenic microalgae cultures to produce compounds with biotechnological and pharmaceutical potential. Also they can be used for cryopreservation and in biochemical, physiology, ecology and genetic studies. The aim of this study was to develop a protocol to decrease the bacterial load in an Isochrysis galbana culture, through washes by centrifugation and the administration of various antibiotics (ampicillin, neomycin, kanamycin, chloramphenicol, sulphate G418, streptomycin, and carbencillin), at several doses and combinations. The concentrations of heterotrophic bacteria and I. galbana cell densities were monitored daily. Maximum non-lethal concentration and lethal concentration 50% (LC50) were calculated. Individually, antibiotics and washes by centrifugation failed to reduce bacterial load, but their combination removed bacteria from the cultures. Peak survival (84.6 ± 1.4%) and reduction of bacterial load in I. galbana cultures were effected with the combination of 5 washes by centrifugation and administration of a cocktail, comprising ampicillin, kanamycin, neomycin, and streptomycin at 48 h. Values of maximum non-lethal concentration varied from 75 to 106 µg mL-1 and LC50 between 194 and 332 µg mL-1, thus, our protocol is an effective and rapid method of producing I. galbana cultures with reduced bacterial load.info:eu-repo/semantics/openAccessUniversidad de Valparaíso. Facultad de Ciencias del MarRevista de biología marina y oceanografía v.51 n.1 20162016-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572016000100010en10.4067/S0718-19572016000100010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Bacterial load Isochrysis galbana antibiotics centrifugation
spellingShingle	Bacterial load Isochrysis galbana antibiotics centrifugation Molina-Cárdenas,Ceres A Sánchez-Saavedra,M. del Pilar Licea-Navarro,Alexei Fedorovish Decreasing of bacterial content in Isochrysis galbana cultures by using some antibiotics
description	The axenic microalgae cultures are a difficult task and they are hard to maintain. Microalgae cultures with reduced bacterial load can be an option to axenic microalgae cultures to produce compounds with biotechnological and pharmaceutical potential. Also they can be used for cryopreservation and in biochemical, physiology, ecology and genetic studies. The aim of this study was to develop a protocol to decrease the bacterial load in an Isochrysis galbana culture, through washes by centrifugation and the administration of various antibiotics (ampicillin, neomycin, kanamycin, chloramphenicol, sulphate G418, streptomycin, and carbencillin), at several doses and combinations. The concentrations of heterotrophic bacteria and I. galbana cell densities were monitored daily. Maximum non-lethal concentration and lethal concentration 50% (LC50) were calculated. Individually, antibiotics and washes by centrifugation failed to reduce bacterial load, but their combination removed bacteria from the cultures. Peak survival (84.6 ± 1.4%) and reduction of bacterial load in I. galbana cultures were effected with the combination of 5 washes by centrifugation and administration of a cocktail, comprising ampicillin, kanamycin, neomycin, and streptomycin at 48 h. Values of maximum non-lethal concentration varied from 75 to 106 µg mL-1 and LC50 between 194 and 332 µg mL-1, thus, our protocol is an effective and rapid method of producing I. galbana cultures with reduced bacterial load.
author	Molina-Cárdenas,Ceres A Sánchez-Saavedra,M. del Pilar Licea-Navarro,Alexei Fedorovish
author_facet	Molina-Cárdenas,Ceres A Sánchez-Saavedra,M. del Pilar Licea-Navarro,Alexei Fedorovish
author_sort	Molina-Cárdenas,Ceres A
title	Decreasing of bacterial content in Isochrysis galbana cultures by using some antibiotics
title_short	Decreasing of bacterial content in Isochrysis galbana cultures by using some antibiotics
title_full	Decreasing of bacterial content in Isochrysis galbana cultures by using some antibiotics
title_fullStr	Decreasing of bacterial content in Isochrysis galbana cultures by using some antibiotics
title_full_unstemmed	Decreasing of bacterial content in Isochrysis galbana cultures by using some antibiotics
title_sort	decreasing of bacterial content in isochrysis galbana cultures by using some antibiotics
publisher	Universidad de Valparaíso. Facultad de Ciencias del Mar
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572016000100010
work_keys_str_mv	AT molinacardenasceresa decreasingofbacterialcontentinisochrysisgalbanaculturesbyusingsomeantibiotics AT sanchezsaavedramdelpilar decreasingofbacterialcontentinisochrysisgalbanaculturesbyusingsomeantibiotics AT liceanavarroalexeifedorovish decreasingofbacterialcontentinisochrysisgalbanaculturesbyusingsomeantibiotics
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Decreasing of bacterial content in Isochrysis galbana cultures by using some antibiotics

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