Nuclear DNA content in Gelidium chilense (Gelidiales, Rhodophyta) from the Chilean coast

There has been progress in novel additions of algal data to the Plant DNA C-values database during recent years; however more information is still required. Specifically, in the case of red algae (Rhodophyta), from ~7000 species described up to date, DNA C-values for only 196 species have been incor...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Salvador-Soler,Noemi, Macaya,Erasmo C, Rull-Lluch,Jordi, Gómez-Garreta,Amelia
Lenguaje:	English
Publicado:	Universidad de Valparaíso. Facultad de Ciencias del Mar 2016
Materias:	DNA content endoreduplication Gelidium life history polyploidy
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572016000100011
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0718-19572016000100011
record_format	dspace
spelling	oai:scielo:S0718-195720160001000112016-06-24Nuclear DNA content in Gelidium chilense (Gelidiales, Rhodophyta) from the Chilean coastSalvador-Soler,NoemiMacaya,Erasmo CRull-Lluch,JordiGómez-Garreta,Amelia DNA content endoreduplication Gelidium life history polyploidy There has been progress in novel additions of algal data to the Plant DNA C-values database during recent years; however more information is still required. Specifically, in the case of red algae (Rhodophyta), from ~7000 species described up to date, DNA C-values for only 196 species have been incorporated (~3%). This research represents the first estimation of genome size for the Southeast Pacific endemic red alga Gelidium chilense and provides nuclear features such as number per cell and size. Nuclear DNA content estimates were obtained from measurements of 153 DAPI-stained nuclei. The cells of G. chilense showed intra-plant variation with DNA content values ranging from 0.2-4.0 pg and a total of 6 ploidy levels were found. The lowest level (1C) was observed only in outer cortical cells whereas tetrasporangia displayed the highest levels (16C). The nuclear DNA contents obtained in tetrasporangia indicated that the genome size increases during tetrasporogenesis by endopolyploidy (from 4C to 16C). In addition, the minimum value observed in tetraspores corresponds to a 3C. Our results confirm the hypothesis that meiosis does not occur within the sporangia in G. chilense. This study contributes to knowledge of reproductive strategies related with the life history of Gelidiales.info:eu-repo/semantics/openAccessUniversidad de Valparaíso. Facultad de Ciencias del MarRevista de biología marina y oceanografía v.51 n.1 20162016-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572016000100011en10.4067/S0718-19572016000100011
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	DNA content endoreduplication Gelidium life history polyploidy
spellingShingle	DNA content endoreduplication Gelidium life history polyploidy Salvador-Soler,Noemi Macaya,Erasmo C Rull-Lluch,Jordi Gómez-Garreta,Amelia Nuclear DNA content in Gelidium chilense (Gelidiales, Rhodophyta) from the Chilean coast
description	There has been progress in novel additions of algal data to the Plant DNA C-values database during recent years; however more information is still required. Specifically, in the case of red algae (Rhodophyta), from ~7000 species described up to date, DNA C-values for only 196 species have been incorporated (~3%). This research represents the first estimation of genome size for the Southeast Pacific endemic red alga Gelidium chilense and provides nuclear features such as number per cell and size. Nuclear DNA content estimates were obtained from measurements of 153 DAPI-stained nuclei. The cells of G. chilense showed intra-plant variation with DNA content values ranging from 0.2-4.0 pg and a total of 6 ploidy levels were found. The lowest level (1C) was observed only in outer cortical cells whereas tetrasporangia displayed the highest levels (16C). The nuclear DNA contents obtained in tetrasporangia indicated that the genome size increases during tetrasporogenesis by endopolyploidy (from 4C to 16C). In addition, the minimum value observed in tetraspores corresponds to a 3C. Our results confirm the hypothesis that meiosis does not occur within the sporangia in G. chilense. This study contributes to knowledge of reproductive strategies related with the life history of Gelidiales.
author	Salvador-Soler,Noemi Macaya,Erasmo C Rull-Lluch,Jordi Gómez-Garreta,Amelia
author_facet	Salvador-Soler,Noemi Macaya,Erasmo C Rull-Lluch,Jordi Gómez-Garreta,Amelia
author_sort	Salvador-Soler,Noemi
title	Nuclear DNA content in Gelidium chilense (Gelidiales, Rhodophyta) from the Chilean coast
title_short	Nuclear DNA content in Gelidium chilense (Gelidiales, Rhodophyta) from the Chilean coast
title_full	Nuclear DNA content in Gelidium chilense (Gelidiales, Rhodophyta) from the Chilean coast
title_fullStr	Nuclear DNA content in Gelidium chilense (Gelidiales, Rhodophyta) from the Chilean coast
title_full_unstemmed	Nuclear DNA content in Gelidium chilense (Gelidiales, Rhodophyta) from the Chilean coast
title_sort	nuclear dna content in gelidium chilense (gelidiales, rhodophyta) from the chilean coast
publisher	Universidad de Valparaíso. Facultad de Ciencias del Mar
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572016000100011
work_keys_str_mv	AT salvadorsolernoemi nucleardnacontentingelidiumchilensegelidialesrhodophytafromthechileancoast AT macayaerasmoc nucleardnacontentingelidiumchilensegelidialesrhodophytafromthechileancoast AT rulllluchjordi nucleardnacontentingelidiumchilensegelidialesrhodophytafromthechileancoast AT gomezgarretaamelia nucleardnacontentingelidiumchilensegelidialesrhodophytafromthechileancoast
_version_	1714202355243679744

Nuclear DNA content in Gelidium chilense (Gelidiales, Rhodophyta) from the Chilean coast

Ejemplares similares