Sea turtle hatchling carapace as a source of high quantity and quality DNA

Hatchling DNA provides valuable information on sea turtles. Samples can be obtained from dead hatchlings or embryo, or, when live animal samples are needed, from blood, flipper or carapace. We compared 120 DNA extractions from flipper and carapace tissue of dead and live hatchlings. There were signi...

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Autores principales:	González-Garza,Blanca I, Sánchez-Teyer,L. Felipe, Zapata-Pérez,Omar
Lenguaje:	English
Publicado:	Universidad de Valparaíso. Facultad de Ciencias del Mar 2016
Materias:	Carapace DNA extraction sea turtle
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572016000100024
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spelling	oai:scielo:S0718-195720160001000242016-06-24Sea turtle hatchling carapace as a source of high quantity and quality DNAGonzález-Garza,Blanca ISánchez-Teyer,L. FelipeZapata-Pérez,Omar Carapace DNA extraction sea turtle Hatchling DNA provides valuable information on sea turtles. Samples can be obtained from dead hatchlings or embryo, or, when live animal samples are needed, from blood, flipper or carapace. We compared 120 DNA extractions from flipper and carapace tissue of dead and live hatchlings. There were significant differences in DNA yields from the different tissues, but no significant differences in DNA purity. Some flipper samples yielded low amounts of DNA, while the carapace tissue consistently produced high yields of good quality DNA. This suggests that carapace represents the best option for tissue sampling of hatchling sea turtles in genetic research.info:eu-repo/semantics/openAccessUniversidad de Valparaíso. Facultad de Ciencias del MarRevista de biología marina y oceanografía v.51 n.1 20162016-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572016000100024en10.4067/S0718-19572016000100024
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Carapace DNA extraction sea turtle
spellingShingle	Carapace DNA extraction sea turtle González-Garza,Blanca I Sánchez-Teyer,L. Felipe Zapata-Pérez,Omar Sea turtle hatchling carapace as a source of high quantity and quality DNA
description	Hatchling DNA provides valuable information on sea turtles. Samples can be obtained from dead hatchlings or embryo, or, when live animal samples are needed, from blood, flipper or carapace. We compared 120 DNA extractions from flipper and carapace tissue of dead and live hatchlings. There were significant differences in DNA yields from the different tissues, but no significant differences in DNA purity. Some flipper samples yielded low amounts of DNA, while the carapace tissue consistently produced high yields of good quality DNA. This suggests that carapace represents the best option for tissue sampling of hatchling sea turtles in genetic research.
author	González-Garza,Blanca I Sánchez-Teyer,L. Felipe Zapata-Pérez,Omar
author_facet	González-Garza,Blanca I Sánchez-Teyer,L. Felipe Zapata-Pérez,Omar
author_sort	González-Garza,Blanca I
title	Sea turtle hatchling carapace as a source of high quantity and quality DNA
title_short	Sea turtle hatchling carapace as a source of high quantity and quality DNA
title_full	Sea turtle hatchling carapace as a source of high quantity and quality DNA
title_fullStr	Sea turtle hatchling carapace as a source of high quantity and quality DNA
title_full_unstemmed	Sea turtle hatchling carapace as a source of high quantity and quality DNA
title_sort	sea turtle hatchling carapace as a source of high quantity and quality dna
publisher	Universidad de Valparaíso. Facultad de Ciencias del Mar
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572016000100024
work_keys_str_mv	AT gonzalezgarzablancai seaturtlehatchlingcarapaceasasourceofhighquantityandqualitydna AT sanchezteyerlfelipe seaturtlehatchlingcarapaceasasourceofhighquantityandqualitydna AT zapataperezomar seaturtlehatchlingcarapaceasasourceofhighquantityandqualitydna
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Sea turtle hatchling carapace as a source of high quantity and quality DNA

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