Molecular cloning, tissue distribution and ontogenetic expression of growth hormone in cobia, Rachycentron canadum

Worldwide, the growth of marine aquaculture is limited by a number of factors. One of the most important is the development of larviculture protocols to produce adequate numbers of juveniles to stock grow-out systems at a convenient cost. In order to develop a tool to assess the nutritional status o...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ibarra-Castro,Leonardo, Webb Jr,Kenneth A, Joan Holt,G
Lenguaje:	English
Publicado:	Universidad de Valparaíso. Facultad de Ciencias del Mar 2016
Materias:	Cobia growth hormone molecular cloning larvae ontogenetic expression
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572016000200018
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0718-19572016000200018
record_format	dspace
spelling	oai:scielo:S0718-195720160002000182016-09-05Molecular cloning, tissue distribution and ontogenetic expression of growth hormone in cobia, Rachycentron canadumIbarra-Castro,LeonardoWebb Jr,Kenneth AJoan Holt,G Cobia growth hormone molecular cloning larvae ontogenetic expression Worldwide, the growth of marine aquaculture is limited by a number of factors. One of the most important is the development of larviculture protocols to produce adequate numbers of juveniles to stock grow-out systems at a convenient cost. In order to develop a tool to assess the nutritional status of cobia larvae, we have cloned and sequenced the Growth Hormone (GH) from the pituitary gland of adult fish, and examined the ontogenetic expression in embryonic and larval specimens by qPCR, as well as tissue distribution in wild adult animals by RT-PCR. The cobia GH sequence showed similarity to the GH sequence of Coryphaena hippurus (mahi mahi), Seriola dumerili (Greater amberjack) and Seriola quinqueradiata (Yellowtail). GH gene expression was studied in 18 different tissues, but was only detected in the pituitary gland, eyes, gill and red muscle. Expression levels were very low in embryos and in early larvae but just before the first feeding, gene expression increased dramatically (~1000 fold) and remained high for the rest of the collection period (to 300 h post fertilization). This pattern of expression is similar to that seen in other rapidly growing temperate marine fish and underscores the rapid somatic growth that begins with the onset of feeding in cobia.info:eu-repo/semantics/openAccessUniversidad de Valparaíso. Facultad de Ciencias del MarRevista de biología marina y oceanografía v.51 n.2 20162016-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572016000200018en10.4067/S0718-19572016000200018
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Cobia growth hormone molecular cloning larvae ontogenetic expression
spellingShingle	Cobia growth hormone molecular cloning larvae ontogenetic expression Ibarra-Castro,Leonardo Webb Jr,Kenneth A Joan Holt,G Molecular cloning, tissue distribution and ontogenetic expression of growth hormone in cobia, Rachycentron canadum
description	Worldwide, the growth of marine aquaculture is limited by a number of factors. One of the most important is the development of larviculture protocols to produce adequate numbers of juveniles to stock grow-out systems at a convenient cost. In order to develop a tool to assess the nutritional status of cobia larvae, we have cloned and sequenced the Growth Hormone (GH) from the pituitary gland of adult fish, and examined the ontogenetic expression in embryonic and larval specimens by qPCR, as well as tissue distribution in wild adult animals by RT-PCR. The cobia GH sequence showed similarity to the GH sequence of Coryphaena hippurus (mahi mahi), Seriola dumerili (Greater amberjack) and Seriola quinqueradiata (Yellowtail). GH gene expression was studied in 18 different tissues, but was only detected in the pituitary gland, eyes, gill and red muscle. Expression levels were very low in embryos and in early larvae but just before the first feeding, gene expression increased dramatically (~1000 fold) and remained high for the rest of the collection period (to 300 h post fertilization). This pattern of expression is similar to that seen in other rapidly growing temperate marine fish and underscores the rapid somatic growth that begins with the onset of feeding in cobia.
author	Ibarra-Castro,Leonardo Webb Jr,Kenneth A Joan Holt,G
author_facet	Ibarra-Castro,Leonardo Webb Jr,Kenneth A Joan Holt,G
author_sort	Ibarra-Castro,Leonardo
title	Molecular cloning, tissue distribution and ontogenetic expression of growth hormone in cobia, Rachycentron canadum
title_short	Molecular cloning, tissue distribution and ontogenetic expression of growth hormone in cobia, Rachycentron canadum
title_full	Molecular cloning, tissue distribution and ontogenetic expression of growth hormone in cobia, Rachycentron canadum
title_fullStr	Molecular cloning, tissue distribution and ontogenetic expression of growth hormone in cobia, Rachycentron canadum
title_full_unstemmed	Molecular cloning, tissue distribution and ontogenetic expression of growth hormone in cobia, Rachycentron canadum
title_sort	molecular cloning, tissue distribution and ontogenetic expression of growth hormone in cobia, rachycentron canadum
publisher	Universidad de Valparaíso. Facultad de Ciencias del Mar
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572016000200018
work_keys_str_mv	AT ibarracastroleonardo molecularcloningtissuedistributionandontogeneticexpressionofgrowthhormoneincobiarachycentroncanadum AT webbjrkennetha molecularcloningtissuedistributionandontogeneticexpressionofgrowthhormoneincobiarachycentroncanadum AT joanholtg molecularcloningtissuedistributionandontogeneticexpressionofgrowthhormoneincobiarachycentroncanadum
_version_	1714202360623923200

Molecular cloning, tissue distribution and ontogenetic expression of growth hormone in cobia, Rachycentron canadum

Ejemplares similares