Acute hyperosmotic stress test for vigor assessment of first-feeding larvae of spotted sand bass Paralabrax maculatofasciatus and spotted rose snapper Lutjanus guttatus

Abstract: This study investigated the tolerance of first-feeding larvae of spotted sand bass Paralabrax maculatofasciatus and spotted rose snapper Lutjanus guttatus to acute hyperosmotic stress. P. maculatofasciatus and L. guttatus embryos were incubated in 48-well microplates at 23 and 28°C, respec...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Martínez-Brown,Juan M., Ibarra-Castro,Leonardo, Rojo-Cebreros,Angel H., López-Cabanillas,Jonathan, Rodríguez-Trejo,Mariana, Ortíz-Galindo,José L.
Lenguaje:	English
Publicado:	Universidad de Valparaíso. Facultad de Ciencias del Mar 2018
Materias:	Larvae quality osmotic stress stress test salinity tolerance Probit analysis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572018000100087
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0718-19572018000100087
record_format	dspace
spelling	oai:scielo:S0718-195720180001000872018-09-11Acute hyperosmotic stress test for vigor assessment of first-feeding larvae of spotted sand bass Paralabrax maculatofasciatus and spotted rose snapper Lutjanus guttatusMartínez-Brown,Juan M.Ibarra-Castro,LeonardoRojo-Cebreros,Angel H.López-Cabanillas,JonathanRodríguez-Trejo,MarianaOrtíz-Galindo,José L. Larvae quality osmotic stress stress test salinity tolerance Probit analysis Abstract: This study investigated the tolerance of first-feeding larvae of spotted sand bass Paralabrax maculatofasciatus and spotted rose snapper Lutjanus guttatus to acute hyperosmotic stress. P. maculatofasciatus and L. guttatus embryos were incubated in 48-well microplates at 23 and 28°C, respectively. The first-feeding larvae were exposed in triplicate to salinities of 35 to 95 g L-1 or 35 to 85 g L-1, and survival was monitored from 0.5-3.5 h or 0.5-53 h, for P. maculatofasciatus and L. guttatus, respectively. The results showed that mortality increased differently among treatments from the first exposure time in each species. Extreme salinities were lethal in 30 min. Salinities close to the control were sub lethal. At intermediate salinities, mortality was fitted to a potential or linear model. For P. maculatofasciatus, the LC50 values obtained between 0.5 and 3.5 h were 73.4 to 65.4 g L-1. For L. guttatus the LC50 values obtained between 2 and 5 h were 57.3 to 54.5 g L-1. Comparison of LC50 between species showed that P. maculatofasciatus larvae had a higher tolerance to hyperosmotic stress. Based on the results for each species, it is recommended to expose to 70 g L-1 for 1 h for P. maculatofasciatus and 57 g L-1 for 2 h for L. guttatus when conducting hyperosmotic stress tests. This stress test is objective, simple, and can be used in both studies on broodstock nutrition and management and in hatcheries as a quality control method.info:eu-repo/semantics/openAccessUniversidad de Valparaíso. Facultad de Ciencias del MarRevista de biología marina y oceanografía v.53 n.1 20182018-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572018000100087en10.4067/S0718-19572018000100087
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Larvae quality osmotic stress stress test salinity tolerance Probit analysis
spellingShingle	Larvae quality osmotic stress stress test salinity tolerance Probit analysis Martínez-Brown,Juan M. Ibarra-Castro,Leonardo Rojo-Cebreros,Angel H. López-Cabanillas,Jonathan Rodríguez-Trejo,Mariana Ortíz-Galindo,José L. Acute hyperosmotic stress test for vigor assessment of first-feeding larvae of spotted sand bass Paralabrax maculatofasciatus and spotted rose snapper Lutjanus guttatus
description	Abstract: This study investigated the tolerance of first-feeding larvae of spotted sand bass Paralabrax maculatofasciatus and spotted rose snapper Lutjanus guttatus to acute hyperosmotic stress. P. maculatofasciatus and L. guttatus embryos were incubated in 48-well microplates at 23 and 28°C, respectively. The first-feeding larvae were exposed in triplicate to salinities of 35 to 95 g L-1 or 35 to 85 g L-1, and survival was monitored from 0.5-3.5 h or 0.5-53 h, for P. maculatofasciatus and L. guttatus, respectively. The results showed that mortality increased differently among treatments from the first exposure time in each species. Extreme salinities were lethal in 30 min. Salinities close to the control were sub lethal. At intermediate salinities, mortality was fitted to a potential or linear model. For P. maculatofasciatus, the LC50 values obtained between 0.5 and 3.5 h were 73.4 to 65.4 g L-1. For L. guttatus the LC50 values obtained between 2 and 5 h were 57.3 to 54.5 g L-1. Comparison of LC50 between species showed that P. maculatofasciatus larvae had a higher tolerance to hyperosmotic stress. Based on the results for each species, it is recommended to expose to 70 g L-1 for 1 h for P. maculatofasciatus and 57 g L-1 for 2 h for L. guttatus when conducting hyperosmotic stress tests. This stress test is objective, simple, and can be used in both studies on broodstock nutrition and management and in hatcheries as a quality control method.
author	Martínez-Brown,Juan M. Ibarra-Castro,Leonardo Rojo-Cebreros,Angel H. López-Cabanillas,Jonathan Rodríguez-Trejo,Mariana Ortíz-Galindo,José L.
author_facet	Martínez-Brown,Juan M. Ibarra-Castro,Leonardo Rojo-Cebreros,Angel H. López-Cabanillas,Jonathan Rodríguez-Trejo,Mariana Ortíz-Galindo,José L.
author_sort	Martínez-Brown,Juan M.
title	Acute hyperosmotic stress test for vigor assessment of first-feeding larvae of spotted sand bass Paralabrax maculatofasciatus and spotted rose snapper Lutjanus guttatus
title_short	Acute hyperosmotic stress test for vigor assessment of first-feeding larvae of spotted sand bass Paralabrax maculatofasciatus and spotted rose snapper Lutjanus guttatus
title_full	Acute hyperosmotic stress test for vigor assessment of first-feeding larvae of spotted sand bass Paralabrax maculatofasciatus and spotted rose snapper Lutjanus guttatus
title_fullStr	Acute hyperosmotic stress test for vigor assessment of first-feeding larvae of spotted sand bass Paralabrax maculatofasciatus and spotted rose snapper Lutjanus guttatus
title_full_unstemmed	Acute hyperosmotic stress test for vigor assessment of first-feeding larvae of spotted sand bass Paralabrax maculatofasciatus and spotted rose snapper Lutjanus guttatus
title_sort	acute hyperosmotic stress test for vigor assessment of first-feeding larvae of spotted sand bass paralabrax maculatofasciatus and spotted rose snapper lutjanus guttatus
publisher	Universidad de Valparaíso. Facultad de Ciencias del Mar
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572018000100087
work_keys_str_mv	AT martinezbrownjuanm acutehyperosmoticstresstestforvigorassessmentoffirstfeedinglarvaeofspottedsandbassparalabraxmaculatofasciatusandspottedrosesnapperlutjanusguttatus AT ibarracastroleonardo acutehyperosmoticstresstestforvigorassessmentoffirstfeedinglarvaeofspottedsandbassparalabraxmaculatofasciatusandspottedrosesnapperlutjanusguttatus AT rojocebrerosangelh acutehyperosmoticstresstestforvigorassessmentoffirstfeedinglarvaeofspottedsandbassparalabraxmaculatofasciatusandspottedrosesnapperlutjanusguttatus AT lopezcabanillasjonathan acutehyperosmoticstresstestforvigorassessmentoffirstfeedinglarvaeofspottedsandbassparalabraxmaculatofasciatusandspottedrosesnapperlutjanusguttatus AT rodrigueztrejomariana acutehyperosmoticstresstestforvigorassessmentoffirstfeedinglarvaeofspottedsandbassparalabraxmaculatofasciatusandspottedrosesnapperlutjanusguttatus AT ortizgalindojosel acutehyperosmoticstresstestforvigorassessmentoffirstfeedinglarvaeofspottedsandbassparalabraxmaculatofasciatusandspottedrosesnapperlutjanusguttatus
_version_	1714202377534308352

Acute hyperosmotic stress test for vigor assessment of first-feeding larvae of spotted sand bass Paralabrax maculatofasciatus and spotted rose snapper Lutjanus guttatus

Ejemplares similares