Antagonistic activity of terrestrial Streptomyces sp. VITNK9 against Gram negative bacterial pathogens affecting the fish and shellfish in aquaculture

Abstract A total of 72 morphologically different actinomycetes isolates were isolated from samples collected at different regions of Vellore, Tamil Nadu, India and screened for its antibacterial activity against fish and shellfish pathogens. All actinomycetes isolates were screened for antibacterial...

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Autores principales:	Ishaque Nabila,Mohammed, Kannabiran,Krishnan
Lenguaje:	English
Publicado:	Universidad de Valparaíso. Facultad de Ciencias del Mar 2018
Materias:	Streptomyces fish and shellfish pathogens antibacterial activity secondary metabolites phylogenetic analysis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572018000200171
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spelling	oai:scielo:S0718-195720180002001712019-01-04Antagonistic activity of terrestrial Streptomyces sp. VITNK9 against Gram negative bacterial pathogens affecting the fish and shellfish in aquacultureIshaque Nabila,MohammedKannabiran,Krishnan Streptomyces fish and shellfish pathogens antibacterial activity secondary metabolites phylogenetic analysis Abstract A total of 72 morphologically different actinomycetes isolates were isolated from samples collected at different regions of Vellore, Tamil Nadu, India and screened for its antibacterial activity against fish and shellfish pathogens. All actinomycetes isolates were screened for antibacterial activity by cross streak method against the selected fish and shellfish bacterial pathogens including Aeromonas caviae, Aeromonas hydroplila, Edwardsiella tarda, Vibrio anguillarum and Vibrio harveyi. Secondary screening of antagonistic isolates by well diffusion method leads to the identification of potential isolate. Culture conditions for the potential isolate were optimized for maximal growth and yield of the ethyl acetate (EA) crude extract. The potential isolate was characterized by molecular taxonomy and phylogeny and identified as Streptomyces species and named as Streptomyces sp. VITNK9. The 16S rDNA nucleotide sequence was searched through the GenBank database and showed 83% similarity to Streptomyces vinaceusdrappus. The EA extract prepared from Streptomyces sp. VITNK9 showed moderate antagonistic activity accessed by the formation of zone of growth inhibition against, Aeromonas caviae (15.33 mm), Aeromonas hydrophila (17.66 mm), Edwardsiella tarda (18.33 mm), Vibrio anguillarum (14.33 mm) and Vibrio harveyi (14.33 mm). The MIC value of EA extract was ranged between 0.03 - 0.125 mg mL-1. The GC-MS spectrum of the ethyl acetate extract revealed the presence of two major compounds, pyrrolo [1,2-A] pyrazine-1,4-Dione (56.67%) and Hexahydro-3-(2-Methylpropyl) (27.91%), respectively. The results of the study suggest that Streptomyces sp. VITNK9 is a potential source for antagonistic secondary metabolites against fish and shellfish bacterial pathogens.info:eu-repo/semantics/openAccessUniversidad de Valparaíso. Facultad de Ciencias del MarRevista de biología marina y oceanografía v.53 n.2 20182018-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572018000200171en10.22370/rbmo.2018.53.2.1291
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Streptomyces fish and shellfish pathogens antibacterial activity secondary metabolites phylogenetic analysis
spellingShingle	Streptomyces fish and shellfish pathogens antibacterial activity secondary metabolites phylogenetic analysis Ishaque Nabila,Mohammed Kannabiran,Krishnan Antagonistic activity of terrestrial Streptomyces sp. VITNK9 against Gram negative bacterial pathogens affecting the fish and shellfish in aquaculture
description	Abstract A total of 72 morphologically different actinomycetes isolates were isolated from samples collected at different regions of Vellore, Tamil Nadu, India and screened for its antibacterial activity against fish and shellfish pathogens. All actinomycetes isolates were screened for antibacterial activity by cross streak method against the selected fish and shellfish bacterial pathogens including Aeromonas caviae, Aeromonas hydroplila, Edwardsiella tarda, Vibrio anguillarum and Vibrio harveyi. Secondary screening of antagonistic isolates by well diffusion method leads to the identification of potential isolate. Culture conditions for the potential isolate were optimized for maximal growth and yield of the ethyl acetate (EA) crude extract. The potential isolate was characterized by molecular taxonomy and phylogeny and identified as Streptomyces species and named as Streptomyces sp. VITNK9. The 16S rDNA nucleotide sequence was searched through the GenBank database and showed 83% similarity to Streptomyces vinaceusdrappus. The EA extract prepared from Streptomyces sp. VITNK9 showed moderate antagonistic activity accessed by the formation of zone of growth inhibition against, Aeromonas caviae (15.33 mm), Aeromonas hydrophila (17.66 mm), Edwardsiella tarda (18.33 mm), Vibrio anguillarum (14.33 mm) and Vibrio harveyi (14.33 mm). The MIC value of EA extract was ranged between 0.03 - 0.125 mg mL-1. The GC-MS spectrum of the ethyl acetate extract revealed the presence of two major compounds, pyrrolo [1,2-A] pyrazine-1,4-Dione (56.67%) and Hexahydro-3-(2-Methylpropyl) (27.91%), respectively. The results of the study suggest that Streptomyces sp. VITNK9 is a potential source for antagonistic secondary metabolites against fish and shellfish bacterial pathogens.
author	Ishaque Nabila,Mohammed Kannabiran,Krishnan
author_facet	Ishaque Nabila,Mohammed Kannabiran,Krishnan
author_sort	Ishaque Nabila,Mohammed
title	Antagonistic activity of terrestrial Streptomyces sp. VITNK9 against Gram negative bacterial pathogens affecting the fish and shellfish in aquaculture
title_short	Antagonistic activity of terrestrial Streptomyces sp. VITNK9 against Gram negative bacterial pathogens affecting the fish and shellfish in aquaculture
title_full	Antagonistic activity of terrestrial Streptomyces sp. VITNK9 against Gram negative bacterial pathogens affecting the fish and shellfish in aquaculture
title_fullStr	Antagonistic activity of terrestrial Streptomyces sp. VITNK9 against Gram negative bacterial pathogens affecting the fish and shellfish in aquaculture
title_full_unstemmed	Antagonistic activity of terrestrial Streptomyces sp. VITNK9 against Gram negative bacterial pathogens affecting the fish and shellfish in aquaculture
title_sort	antagonistic activity of terrestrial streptomyces sp. vitnk9 against gram negative bacterial pathogens affecting the fish and shellfish in aquaculture
publisher	Universidad de Valparaíso. Facultad de Ciencias del Mar
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572018000200171
work_keys_str_mv	AT ishaquenabilamohammed antagonisticactivityofterrestrialstreptomycesspvitnk9againstgramnegativebacterialpathogensaffectingthefishandshellfishinaquaculture AT kannabirankrishnan antagonisticactivityofterrestrialstreptomycesspvitnk9againstgramnegativebacterialpathogensaffectingthefishandshellfishinaquaculture
_version_	1714202378863902720

Antagonistic activity of terrestrial Streptomyces sp. VITNK9 against Gram negative bacterial pathogens affecting the fish and shellfish in aquaculture

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