Development of six novel microsatellite markers for the Chilean red alga Pyropia orbicularis

Abstract: Mariculture of edible Porphyra/Pyropia species, which are cosmopolitan red foliose algae, represent an important source of income for the pharmaceutical and food industries. In Chile, the most common alga of this complex is Pyropia orbicularis (Bangiales, Rhodophyta). Here we report 6 micr...

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Autores principales:	Pérez-Lara,Gabriel, Meynard,Andrés, Alvear,Paula, Bulboa-Contador,Cristian, Contreras-Porcia,Loretto
Lenguaje:	English
Publicado:	Universidad de Valparaíso. Facultad de Ciencias del Mar 2020
Materias:	Pyropia orbicularis microsatellite markers Rhodophyta
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572020000100085
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spelling	oai:scielo:S0718-195720200001000852020-09-11Development of six novel microsatellite markers for the Chilean red alga Pyropia orbicularisPérez-Lara,GabrielMeynard,AndrésAlvear,PaulaBulboa-Contador,CristianContreras-Porcia,Loretto Pyropia orbicularis microsatellite markers Rhodophyta Abstract: Mariculture of edible Porphyra/Pyropia species, which are cosmopolitan red foliose algae, represent an important source of income for the pharmaceutical and food industries. In Chile, the most common alga of this complex is Pyropia orbicularis (Bangiales, Rhodophyta). Here we report 6 microsatellite markers obtained from P. orbicularis through a genomic library from 10 individuals of the gametophytic phase by next generation (Illumina) sequencing. Polymorphism analyses were done from 10 individuals of the conchocelis phase, revealing an allelic diversity ranging from three to six alleles per locus, and observed heterozygosity (HO) and expected heterozygosity (HE) ranging from 0.200 to 0.995, and 0.250 to 0.610, respectively. A large proportion of genetic variance (61%) in P. orbicularis was among individuals within population, and 39% genetic variance was among populations. Nonetheless, these results should be interpreted with caution; further research using larger sample sizes is required. These polymorphic markers could be useful in future studies on population genetic structure, for conservation and applied purposes.info:eu-repo/semantics/openAccessUniversidad de Valparaíso. Facultad de Ciencias del MarRevista de biología marina y oceanografía v.55 n.1 20202020-08-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572020000100085en10.22370/rbmo.2020.55.1.2354
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Pyropia orbicularis microsatellite markers Rhodophyta
spellingShingle	Pyropia orbicularis microsatellite markers Rhodophyta Pérez-Lara,Gabriel Meynard,Andrés Alvear,Paula Bulboa-Contador,Cristian Contreras-Porcia,Loretto Development of six novel microsatellite markers for the Chilean red alga Pyropia orbicularis
description	Abstract: Mariculture of edible Porphyra/Pyropia species, which are cosmopolitan red foliose algae, represent an important source of income for the pharmaceutical and food industries. In Chile, the most common alga of this complex is Pyropia orbicularis (Bangiales, Rhodophyta). Here we report 6 microsatellite markers obtained from P. orbicularis through a genomic library from 10 individuals of the gametophytic phase by next generation (Illumina) sequencing. Polymorphism analyses were done from 10 individuals of the conchocelis phase, revealing an allelic diversity ranging from three to six alleles per locus, and observed heterozygosity (HO) and expected heterozygosity (HE) ranging from 0.200 to 0.995, and 0.250 to 0.610, respectively. A large proportion of genetic variance (61%) in P. orbicularis was among individuals within population, and 39% genetic variance was among populations. Nonetheless, these results should be interpreted with caution; further research using larger sample sizes is required. These polymorphic markers could be useful in future studies on population genetic structure, for conservation and applied purposes.
author	Pérez-Lara,Gabriel Meynard,Andrés Alvear,Paula Bulboa-Contador,Cristian Contreras-Porcia,Loretto
author_facet	Pérez-Lara,Gabriel Meynard,Andrés Alvear,Paula Bulboa-Contador,Cristian Contreras-Porcia,Loretto
author_sort	Pérez-Lara,Gabriel
title	Development of six novel microsatellite markers for the Chilean red alga Pyropia orbicularis
title_short	Development of six novel microsatellite markers for the Chilean red alga Pyropia orbicularis
title_full	Development of six novel microsatellite markers for the Chilean red alga Pyropia orbicularis
title_fullStr	Development of six novel microsatellite markers for the Chilean red alga Pyropia orbicularis
title_full_unstemmed	Development of six novel microsatellite markers for the Chilean red alga Pyropia orbicularis
title_sort	development of six novel microsatellite markers for the chilean red alga pyropia orbicularis
publisher	Universidad de Valparaíso. Facultad de Ciencias del Mar
publishDate	2020
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-19572020000100085
work_keys_str_mv	AT perezlaragabriel developmentofsixnovelmicrosatellitemarkersforthechileanredalgapyropiaorbicularis AT meynardandres developmentofsixnovelmicrosatellitemarkersforthechileanredalgapyropiaorbicularis AT alvearpaula developmentofsixnovelmicrosatellitemarkersforthechileanredalgapyropiaorbicularis AT bulboacontadorcristian developmentofsixnovelmicrosatellitemarkersforthechileanredalgapyropiaorbicularis AT contrerasporcialoretto developmentofsixnovelmicrosatellitemarkersforthechileanredalgapyropiaorbicularis
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Development of six novel microsatellite markers for the Chilean red alga Pyropia orbicularis

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