Developing a model system in vitro to understand tracheary element development in Douglas-fir (Pseudostuga mensziesii)

Callus cells were initiated on cambial strips obtained from 4 to 8 y old Douglas-fir (Pseudostuga menziesii) trees, cultured on solidified Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyaceticacid (2,4-D) and benzylaminopurine (BA). The cultures could be maintained by sub-cultu...

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Autores principales:	Pillai,Karthik V, McDonald,Armando G, Wagner,Francis G
Lenguaje:	English
Publicado:	Universidad del Bío-Bío 2011
Materias:	Callus culture cell differentiation Douglas-fir plant cell walls tracheary elements
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-221X2011000100001
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spelling	oai:scielo:S0718-221X20110001000012011-05-30Developing a model system in vitro to understand tracheary element development in Douglas-fir (Pseudostuga mensziesii)Pillai,Karthik VMcDonald,Armando GWagner,Francis G Callus culture cell differentiation Douglas-fir plant cell walls tracheary elements Callus cells were initiated on cambial strips obtained from 4 to 8 y old Douglas-fir (Pseudostuga menziesii) trees, cultured on solidified Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyaceticacid (2,4-D) and benzylaminopurine (BA). The cultures could be maintained by sub-culturing on fresh medium every four weeks. When the callus cells were subsequently transferred to liquid MS medium supplemented with different phytohormones, suspension cultures could be initiated and maintained by periodic sub-culture. Approximately 65% of the callus cells cultured on liquid MS medium supplemented with 2,4-D, when maintained for 6-7 weeks without sub-culture, differentiated to tracheary element (TE) like cells. The formation of TE like cells was confirmed histochemically by staining with phloroglucinol-HCl. Secondary thickening of the cell walls were confirmed by polarized light microscopy, which showed strong birefringence of the cell wall due the presence of crystalline cellulose. The presence of lignin was determined by pyrolysis-GC-MS and FTIR spectroscopy. The lignin content in differentiated cell wall samples was quantified at 21% by the lignothioglycolic acid assay. Analysis of monosaccharide composition of cell wall samples after acid hydrolysis showed that the percentage of glucose, xylose and mannose had increased in the differentiated cell walls. These increases correspond to the formation of cellulose, glucomannan and xylan, primarily associated with secondary cell walls.info:eu-repo/semantics/openAccessUniversidad del Bío-BíoMaderas. Ciencia y tecnología v.13 n.1 20112011-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-221X2011000100001en10.4067/S0718-221X2011000100001
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Callus culture cell differentiation Douglas-fir plant cell walls tracheary elements
spellingShingle	Callus culture cell differentiation Douglas-fir plant cell walls tracheary elements Pillai,Karthik V McDonald,Armando G Wagner,Francis G Developing a model system in vitro to understand tracheary element development in Douglas-fir (Pseudostuga mensziesii)
description	Callus cells were initiated on cambial strips obtained from 4 to 8 y old Douglas-fir (Pseudostuga menziesii) trees, cultured on solidified Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyaceticacid (2,4-D) and benzylaminopurine (BA). The cultures could be maintained by sub-culturing on fresh medium every four weeks. When the callus cells were subsequently transferred to liquid MS medium supplemented with different phytohormones, suspension cultures could be initiated and maintained by periodic sub-culture. Approximately 65% of the callus cells cultured on liquid MS medium supplemented with 2,4-D, when maintained for 6-7 weeks without sub-culture, differentiated to tracheary element (TE) like cells. The formation of TE like cells was confirmed histochemically by staining with phloroglucinol-HCl. Secondary thickening of the cell walls were confirmed by polarized light microscopy, which showed strong birefringence of the cell wall due the presence of crystalline cellulose. The presence of lignin was determined by pyrolysis-GC-MS and FTIR spectroscopy. The lignin content in differentiated cell wall samples was quantified at 21% by the lignothioglycolic acid assay. Analysis of monosaccharide composition of cell wall samples after acid hydrolysis showed that the percentage of glucose, xylose and mannose had increased in the differentiated cell walls. These increases correspond to the formation of cellulose, glucomannan and xylan, primarily associated with secondary cell walls.
author	Pillai,Karthik V McDonald,Armando G Wagner,Francis G
author_facet	Pillai,Karthik V McDonald,Armando G Wagner,Francis G
author_sort	Pillai,Karthik V
title	Developing a model system in vitro to understand tracheary element development in Douglas-fir (Pseudostuga mensziesii)
title_short	Developing a model system in vitro to understand tracheary element development in Douglas-fir (Pseudostuga mensziesii)
title_full	Developing a model system in vitro to understand tracheary element development in Douglas-fir (Pseudostuga mensziesii)
title_fullStr	Developing a model system in vitro to understand tracheary element development in Douglas-fir (Pseudostuga mensziesii)
title_full_unstemmed	Developing a model system in vitro to understand tracheary element development in Douglas-fir (Pseudostuga mensziesii)
title_sort	developing a model system in vitro to understand tracheary element development in douglas-fir (pseudostuga mensziesii)
publisher	Universidad del Bío-Bío
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-221X2011000100001
work_keys_str_mv	AT pillaikarthikv developingamodelsysteminvitrotounderstandtrachearyelementdevelopmentindouglasfirpseudostugamensziesii AT mcdonaldarmandog developingamodelsysteminvitrotounderstandtrachearyelementdevelopmentindouglasfirpseudostugamensziesii AT wagnerfrancisg developingamodelsysteminvitrotounderstandtrachearyelementdevelopmentindouglasfirpseudostugamensziesii
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Developing a model system in vitro to understand tracheary element development in Douglas-fir (Pseudostuga mensziesii)

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