Phenolics content and antioxidant activity of wood extractives from three clones of Acacia hybrid (Acacia mangium × Acacia auriculiformis)

Abstract: Breeding strategy of Acacia hybrid is being developed by Center for Forest Biotechnology and Tree Improvement Indonesia and has produced three superior clones in growth (Clone 16, 25, and 44). Understanding the extractives of the new clones might determine future development steps to impro...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Verick Purba,Brandon Aristo, Sunarti,Sri, Lukmandaru,Ganis
Lenguaje:	English
Publicado:	Universidad del Bío-Bío 2021
Materias:	Acacia hybrid heartwood phenolic contents sapwood wood extractives.
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-221X2021000100428
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0718-221X2021000100428
record_format	dspace
spelling	oai:scielo:S0718-221X20210001004282021-05-30Phenolics content and antioxidant activity of wood extractives from three clones of Acacia hybrid (Acacia mangium × Acacia auriculiformis)Verick Purba,Brandon AristoSunarti,SriLukmandaru,Ganis Acacia hybrid heartwood phenolic contents sapwood wood extractives. Abstract: Breeding strategy of Acacia hybrid is being developed by Center for Forest Biotechnology and Tree Improvement Indonesia and has produced three superior clones in growth (Clone 16, 25, and 44). Understanding the extractives of the new clones might determine future development steps to improve its resistance to diseases especially heartrot. The objective of this study was to investigate the extractive content in three radial directions (SW = sapwood; OHW = outer heartwood; IHW = inner heartwood); total phenolic, flavonoid, flavanol contents (colorimetric assay); and antioxidant activity (1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay). Extractions were done with three different solvents in successive (n-hexane = H; methanol = M; hot water = W), yielded 0,69 % - 1,70 %; 1,51 % - 10,86 %; and 0,51 % - 1,16 % of extractive contents, respectively. The total phenolic content (TPC) from TPC-H, TPC-M, and TPC-W ranged between 3,68 mg of gallic acid equivalent (GAE)/g - 10,41 mg GAE/g; 76,83 mg GAE/g - 448,35 mg GAE/g; and 43,28 mg GAE/g - 198,92 mg GAE/g, respectively; the total flavonoid content (TFC) from TFC-H, TFC-M, and TFC-W between 4,23 mg of quercetin equivalent (QE)/g - 41,51 mg QE/g; 29,55 mg QE/g - 133,71 mg QE/g; and 7,70 mg QE/g - 29,37 mg QE/g, respectively; total flavanol content (TVC) from TVC-H, TVC-M, and TVC-W ranged between 28,74 mg of catechin equivalent (CE)/g - 66,90 mg CE/g; 83,39 mg CE/g - 247,18 mg CE/g; and 7,08 mg CE/g - 29,21 mg CE/g, respectively. Furthermore, the antioxidant activity was found to be significantly affected by the radial factor with the strongest activity exhibited by inner heartwood extract with an IC50 value of 255,77 μg/ml (gallic acid IC50 showed a value of 39,00 μg/ml). Among clones, clone 16 was determined to have the highest extractive, total flavonoid as well as flavanol contents. Thus, clone 16 was hypothesized to be more resistance against heart rot disease.info:eu-repo/semantics/openAccessUniversidad del Bío-BíoMaderas. Ciencia y tecnología v.23 20212021-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-221X2021000100428en10.4067/s0718-221x2021000100428
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Acacia hybrid heartwood phenolic contents sapwood wood extractives.
spellingShingle	Acacia hybrid heartwood phenolic contents sapwood wood extractives. Verick Purba,Brandon Aristo Sunarti,Sri Lukmandaru,Ganis Phenolics content and antioxidant activity of wood extractives from three clones of Acacia hybrid (Acacia mangium × Acacia auriculiformis)
description	Abstract: Breeding strategy of Acacia hybrid is being developed by Center for Forest Biotechnology and Tree Improvement Indonesia and has produced three superior clones in growth (Clone 16, 25, and 44). Understanding the extractives of the new clones might determine future development steps to improve its resistance to diseases especially heartrot. The objective of this study was to investigate the extractive content in three radial directions (SW = sapwood; OHW = outer heartwood; IHW = inner heartwood); total phenolic, flavonoid, flavanol contents (colorimetric assay); and antioxidant activity (1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay). Extractions were done with three different solvents in successive (n-hexane = H; methanol = M; hot water = W), yielded 0,69 % - 1,70 %; 1,51 % - 10,86 %; and 0,51 % - 1,16 % of extractive contents, respectively. The total phenolic content (TPC) from TPC-H, TPC-M, and TPC-W ranged between 3,68 mg of gallic acid equivalent (GAE)/g - 10,41 mg GAE/g; 76,83 mg GAE/g - 448,35 mg GAE/g; and 43,28 mg GAE/g - 198,92 mg GAE/g, respectively; the total flavonoid content (TFC) from TFC-H, TFC-M, and TFC-W between 4,23 mg of quercetin equivalent (QE)/g - 41,51 mg QE/g; 29,55 mg QE/g - 133,71 mg QE/g; and 7,70 mg QE/g - 29,37 mg QE/g, respectively; total flavanol content (TVC) from TVC-H, TVC-M, and TVC-W ranged between 28,74 mg of catechin equivalent (CE)/g - 66,90 mg CE/g; 83,39 mg CE/g - 247,18 mg CE/g; and 7,08 mg CE/g - 29,21 mg CE/g, respectively. Furthermore, the antioxidant activity was found to be significantly affected by the radial factor with the strongest activity exhibited by inner heartwood extract with an IC50 value of 255,77 μg/ml (gallic acid IC50 showed a value of 39,00 μg/ml). Among clones, clone 16 was determined to have the highest extractive, total flavonoid as well as flavanol contents. Thus, clone 16 was hypothesized to be more resistance against heart rot disease.
author	Verick Purba,Brandon Aristo Sunarti,Sri Lukmandaru,Ganis
author_facet	Verick Purba,Brandon Aristo Sunarti,Sri Lukmandaru,Ganis
author_sort	Verick Purba,Brandon Aristo
title	Phenolics content and antioxidant activity of wood extractives from three clones of Acacia hybrid (Acacia mangium × Acacia auriculiformis)
title_short	Phenolics content and antioxidant activity of wood extractives from three clones of Acacia hybrid (Acacia mangium × Acacia auriculiformis)
title_full	Phenolics content and antioxidant activity of wood extractives from three clones of Acacia hybrid (Acacia mangium × Acacia auriculiformis)
title_fullStr	Phenolics content and antioxidant activity of wood extractives from three clones of Acacia hybrid (Acacia mangium × Acacia auriculiformis)
title_full_unstemmed	Phenolics content and antioxidant activity of wood extractives from three clones of Acacia hybrid (Acacia mangium × Acacia auriculiformis)
title_sort	phenolics content and antioxidant activity of wood extractives from three clones of acacia hybrid (acacia mangium × acacia auriculiformis)
publisher	Universidad del Bío-Bío
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-221X2021000100428
work_keys_str_mv	AT verickpurbabrandonaristo phenolicscontentandantioxidantactivityofwoodextractivesfromthreeclonesofacaciahybridacaciamangiumacaciaauriculiformis AT sunartisri phenolicscontentandantioxidantactivityofwoodextractivesfromthreeclonesofacaciahybridacaciamangiumacaciaauriculiformis AT lukmandaruganis phenolicscontentandantioxidantactivityofwoodextractivesfromthreeclonesofacaciahybridacaciamangiumacaciaauriculiformis
_version_	1714202701637615616

Phenolics content and antioxidant activity of wood extractives from three clones of Acacia hybrid (Acacia mangium × Acacia auriculiformis)

Ejemplares similares