MODERN APPROACHES FOR THE STUDY OF s-TRIAZINE HERBICIDE BIOREMEDIATION IN AGRICULTURAL SOILS

The extensive use of s-triazine herbicides in diverse countries causes environmental and health concern. Simazine and atrazine are s-triazines widely used in agriculture and forestry. Although, natural dissipation of s-triazines in soils by physicochemical processes has been described, the main mech...

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Autores principales:	Hernández,Marcela, Morgante,Verónica, Flores,Cecilia, Villalobos,Patricio, González,Myriam, Miralles,Pola, Dinamarca,Alejandro, Seeger,Michael
Lenguaje:	English
Publicado:	Sociedad Chilena de la Ciencia del Suelo 2008
Materias:	í-triazine simazine biodegradation bioremediation herbicide
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-27912008000200004
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spelling	oai:scielo:S0718-279120080002000042009-12-23MODERN APPROACHES FOR THE STUDY OF s-TRIAZINE HERBICIDE BIOREMEDIATION IN AGRICULTURAL SOILSHernández,MarcelaMorgante,VerónicaFlores,CeciliaVillalobos,PatricioGonzález,MyriamMiralles,PolaDinamarca,AlejandroSeeger,Michael í-triazine simazine biodegradation bioremediation herbicide The extensive use of s-triazine herbicides in diverse countries causes environmental and health concern. Simazine and atrazine are s-triazines widely used in agriculture and forestry. Although, natural dissipation of s-triazines in soils by physicochemical processes has been described, the main mechanism for their removal is biological degradation by microorganisms. Bioremediation is a successful strategy for the removal of i-triazines in soil. For bioaugmentation processes, s-triazine-degrading bacteria are required, which isolation from agricultural soils was described in this report. Studies of s-triazine adsorption and leaching in soil are useful to determine the bioavailability of these herbicides. The detection of s-triazine-degrading catabolic activity by most-probable-number (MPN) and the reduction of the respiration indicator 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) were presented. The relative abundances of s-triazine catabolic genes in soil were analyzed by the MPN-PCR technique. Culture-independent molecular methods such as FISH, T-RFLP and clone libraries are useful to study the effects of herbicide application and bioaugmentation on soil microbial communities and their dynamics. These experimental methods allow the design of biotechnological strategies for the clean-up of s-triazine contaminated soils.info:eu-repo/semantics/openAccessSociedad Chilena de la Ciencia del SueloRevista de la ciencia del suelo y nutrición vegetal v.8 n.2 20082008-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-27912008000200004en10.4067/S0718-27912008000200004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	í-triazine simazine biodegradation bioremediation herbicide
spellingShingle	í-triazine simazine biodegradation bioremediation herbicide Hernández,Marcela Morgante,Verónica Flores,Cecilia Villalobos,Patricio González,Myriam Miralles,Pola Dinamarca,Alejandro Seeger,Michael MODERN APPROACHES FOR THE STUDY OF s-TRIAZINE HERBICIDE BIOREMEDIATION IN AGRICULTURAL SOILS
description	The extensive use of s-triazine herbicides in diverse countries causes environmental and health concern. Simazine and atrazine are s-triazines widely used in agriculture and forestry. Although, natural dissipation of s-triazines in soils by physicochemical processes has been described, the main mechanism for their removal is biological degradation by microorganisms. Bioremediation is a successful strategy for the removal of i-triazines in soil. For bioaugmentation processes, s-triazine-degrading bacteria are required, which isolation from agricultural soils was described in this report. Studies of s-triazine adsorption and leaching in soil are useful to determine the bioavailability of these herbicides. The detection of s-triazine-degrading catabolic activity by most-probable-number (MPN) and the reduction of the respiration indicator 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) were presented. The relative abundances of s-triazine catabolic genes in soil were analyzed by the MPN-PCR technique. Culture-independent molecular methods such as FISH, T-RFLP and clone libraries are useful to study the effects of herbicide application and bioaugmentation on soil microbial communities and their dynamics. These experimental methods allow the design of biotechnological strategies for the clean-up of s-triazine contaminated soils.
author	Hernández,Marcela Morgante,Verónica Flores,Cecilia Villalobos,Patricio González,Myriam Miralles,Pola Dinamarca,Alejandro Seeger,Michael
author_facet	Hernández,Marcela Morgante,Verónica Flores,Cecilia Villalobos,Patricio González,Myriam Miralles,Pola Dinamarca,Alejandro Seeger,Michael
author_sort	Hernández,Marcela
title	MODERN APPROACHES FOR THE STUDY OF s-TRIAZINE HERBICIDE BIOREMEDIATION IN AGRICULTURAL SOILS
title_short	MODERN APPROACHES FOR THE STUDY OF s-TRIAZINE HERBICIDE BIOREMEDIATION IN AGRICULTURAL SOILS
title_full	MODERN APPROACHES FOR THE STUDY OF s-TRIAZINE HERBICIDE BIOREMEDIATION IN AGRICULTURAL SOILS
title_fullStr	MODERN APPROACHES FOR THE STUDY OF s-TRIAZINE HERBICIDE BIOREMEDIATION IN AGRICULTURAL SOILS
title_full_unstemmed	MODERN APPROACHES FOR THE STUDY OF s-TRIAZINE HERBICIDE BIOREMEDIATION IN AGRICULTURAL SOILS
title_sort	modern approaches for the study of s-triazine herbicide bioremediation in agricultural soils
publisher	Sociedad Chilena de la Ciencia del Suelo
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-27912008000200004
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MODERN APPROACHES FOR THE STUDY OF s-TRIAZINE HERBICIDE BIOREMEDIATION IN AGRICULTURAL SOILS

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