A fast and efficient method for total DNA extraction from soil filamentous fungi
Extraction of high quality of DNA from fungus can be affected because the presence of the complex cell wall, high content of polysaccharides or secondary metabolites. In this research, we adapt a simple method of DNA extraction and purification of from fungus isolated from soil for Polymerase Chain...
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Universidad de Tarapacá. Facultad de Ciencias Agronómicas
2014
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oai:scielo:S0718-342920140002000102014-06-25A fast and efficient method for total DNA extraction from soil filamentous fungiHuanca-Mamani,WilsonSalvatierra Martínez,RicardoSepúlveda-Chavera,Germán DNA extraction fungi soil PCR Extraction of high quality of DNA from fungus can be affected because the presence of the complex cell wall, high content of polysaccharides or secondary metabolites. In this research, we adapt a simple method of DNA extraction and purification of from fungus isolated from soil for Polymerase Chain Reaction (PCR) applications. The methodology described is both rapid and cost effective.info:eu-repo/semantics/openAccessUniversidad de Tarapacá. Facultad de Ciencias AgronómicasIdesia (Arica) v.32 n.2 20142014-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-34292014000200010en10.4067/S0718-34292014000200010 |
institution |
Scielo Chile |
collection |
Scielo Chile |
language |
English |
topic |
DNA extraction fungi soil PCR |
spellingShingle |
DNA extraction fungi soil PCR Huanca-Mamani,Wilson Salvatierra Martínez,Ricardo Sepúlveda-Chavera,Germán A fast and efficient method for total DNA extraction from soil filamentous fungi |
description |
Extraction of high quality of DNA from fungus can be affected because the presence of the complex cell wall, high content of polysaccharides or secondary metabolites. In this research, we adapt a simple method of DNA extraction and purification of from fungus isolated from soil for Polymerase Chain Reaction (PCR) applications. The methodology described is both rapid and cost effective. |
author |
Huanca-Mamani,Wilson Salvatierra Martínez,Ricardo Sepúlveda-Chavera,Germán |
author_facet |
Huanca-Mamani,Wilson Salvatierra Martínez,Ricardo Sepúlveda-Chavera,Germán |
author_sort |
Huanca-Mamani,Wilson |
title |
A fast and efficient method for total DNA extraction from soil filamentous fungi |
title_short |
A fast and efficient method for total DNA extraction from soil filamentous fungi |
title_full |
A fast and efficient method for total DNA extraction from soil filamentous fungi |
title_fullStr |
A fast and efficient method for total DNA extraction from soil filamentous fungi |
title_full_unstemmed |
A fast and efficient method for total DNA extraction from soil filamentous fungi |
title_sort |
fast and efficient method for total dna extraction from soil filamentous fungi |
publisher |
Universidad de Tarapacá. Facultad de Ciencias Agronómicas |
publishDate |
2014 |
url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-34292014000200010 |
work_keys_str_mv |
AT huancamamaniwilson afastandefficientmethodfortotaldnaextractionfromsoilfilamentousfungi AT salvatierramartinezricardo afastandefficientmethodfortotaldnaextractionfromsoilfilamentousfungi AT sepulvedachaveragerman afastandefficientmethodfortotaldnaextractionfromsoilfilamentousfungi AT huancamamaniwilson fastandefficientmethodfortotaldnaextractionfromsoilfilamentousfungi AT salvatierramartinezricardo fastandefficientmethodfortotaldnaextractionfromsoilfilamentousfungi AT sepulvedachaveragerman fastandefficientmethodfortotaldnaextractionfromsoilfilamentousfungi |
_version_ |
1714203697930567680 |