A fast and efficient method for total DNA extraction from soil filamentous fungi

Extraction of high quality of DNA from fungus can be affected because the presence of the complex cell wall, high content of polysaccharides or secondary metabolites. In this research, we adapt a simple method of DNA extraction and purification of from fungus isolated from soil for Polymerase Chain...

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Autores principales: Huanca-Mamani,Wilson, Salvatierra Martínez,Ricardo, Sepúlveda-Chavera,Germán
Lenguaje:English
Publicado: Universidad de Tarapacá. Facultad de Ciencias Agronómicas 2014
Materias:
PCR
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-34292014000200010
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spelling oai:scielo:S0718-342920140002000102014-06-25A fast and efficient method for total DNA extraction from soil filamentous fungiHuanca-Mamani,WilsonSalvatierra Martínez,RicardoSepúlveda-Chavera,Germán DNA extraction fungi soil PCR Extraction of high quality of DNA from fungus can be affected because the presence of the complex cell wall, high content of polysaccharides or secondary metabolites. In this research, we adapt a simple method of DNA extraction and purification of from fungus isolated from soil for Polymerase Chain Reaction (PCR) applications. The methodology described is both rapid and cost effective.info:eu-repo/semantics/openAccessUniversidad de Tarapacá. Facultad de Ciencias AgronómicasIdesia (Arica) v.32 n.2 20142014-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-34292014000200010en10.4067/S0718-34292014000200010
institution Scielo Chile
collection Scielo Chile
language English
topic DNA extraction
fungi
soil
PCR
spellingShingle DNA extraction
fungi
soil
PCR
Huanca-Mamani,Wilson
Salvatierra Martínez,Ricardo
Sepúlveda-Chavera,Germán
A fast and efficient method for total DNA extraction from soil filamentous fungi
description Extraction of high quality of DNA from fungus can be affected because the presence of the complex cell wall, high content of polysaccharides or secondary metabolites. In this research, we adapt a simple method of DNA extraction and purification of from fungus isolated from soil for Polymerase Chain Reaction (PCR) applications. The methodology described is both rapid and cost effective.
author Huanca-Mamani,Wilson
Salvatierra Martínez,Ricardo
Sepúlveda-Chavera,Germán
author_facet Huanca-Mamani,Wilson
Salvatierra Martínez,Ricardo
Sepúlveda-Chavera,Germán
author_sort Huanca-Mamani,Wilson
title A fast and efficient method for total DNA extraction from soil filamentous fungi
title_short A fast and efficient method for total DNA extraction from soil filamentous fungi
title_full A fast and efficient method for total DNA extraction from soil filamentous fungi
title_fullStr A fast and efficient method for total DNA extraction from soil filamentous fungi
title_full_unstemmed A fast and efficient method for total DNA extraction from soil filamentous fungi
title_sort fast and efficient method for total dna extraction from soil filamentous fungi
publisher Universidad de Tarapacá. Facultad de Ciencias Agronómicas
publishDate 2014
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-34292014000200010
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