ISSR molecular markers for the study of the genetic diversity of Mimosa caesalpiniaefolia Benth.

Knowledge of the genetic diversity of the threatened tree species in the Brazilian semiarid region may contribute to the creation of effective strategies for their preservation and future use. The aim of this study was to select ISSR (Inter-Simple Sequence Repeat) primers capable of detecting geneti...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	dos Santos Araújo,Fernando, Vasconcelos Pacheco,Mauro, de Almeida Vieira,Fábio, dos Santos Ferrari,Cibele, Cardoso Félix,Francival, Teixeira das Chagas,Kyvia Pontes
Lenguaje:	English
Publicado:	Universidad de Tarapacá. Facultad de Ciencias Agronómicas 2016
Materias:	semiarid forest species primers polymorphism
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-34292016000300007
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0718-34292016000300007
record_format	dspace
spelling	oai:scielo:S0718-342920160003000072016-07-01ISSR molecular markers for the study of the genetic diversity of Mimosa caesalpiniaefolia Benth.dos Santos Araújo,FernandoVasconcelos Pacheco,Maurode Almeida Vieira,Fábiodos Santos Ferrari,CibeleCardoso Félix,FrancivalTeixeira das Chagas,Kyvia Pontes semiarid forest species primers polymorphism Knowledge of the genetic diversity of the threatened tree species in the Brazilian semiarid region may contribute to the creation of effective strategies for their preservation and future use. The aim of this study was to select ISSR (Inter-Simple Sequence Repeat) primers capable of detecting genetic polymorphism among Mimosa caesalpiniaefolia Benth. individuals for a study of the genetic diversity of this species. A total of 27 ISSR primers were tested for their ability to amplify genomic DNA of three individuals (bulk); all of them amplified regions between the simple sequence repeats of the genome, however seven ISSR primers showed a good standard of amplification compared to the others. The seven primers selected were used to amplify the genomic DNA of nine individuals and generated a total of 78 loci, of which 52.7% were polymorphic. The content of polymorphic information of the primers ranged from 0.261 to 0.489, showing that selected primers are moderately informative; bootstrap analysis determined that 51 loci were enough to estimate the genetic diversity of the samples of individuals. We concluded that the markers generated by the primers UBC 807, UBC 824, UBC 827, UBC 840, UBC 851, UBC 873 and UBC 881 allow detection of the genetic polymorphism among individuals of M. caesalpiniaefolia, being useful to determine the genetic diversity of this species.info:eu-repo/semantics/openAccessUniversidad de Tarapacá. Facultad de Ciencias AgronómicasIdesia (Arica) v.34 n.3 20162016-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-34292016000300007en10.4067/S0718-34292016000300007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	semiarid forest species primers polymorphism
spellingShingle	semiarid forest species primers polymorphism dos Santos Araújo,Fernando Vasconcelos Pacheco,Mauro de Almeida Vieira,Fábio dos Santos Ferrari,Cibele Cardoso Félix,Francival Teixeira das Chagas,Kyvia Pontes ISSR molecular markers for the study of the genetic diversity of Mimosa caesalpiniaefolia Benth.
description	Knowledge of the genetic diversity of the threatened tree species in the Brazilian semiarid region may contribute to the creation of effective strategies for their preservation and future use. The aim of this study was to select ISSR (Inter-Simple Sequence Repeat) primers capable of detecting genetic polymorphism among Mimosa caesalpiniaefolia Benth. individuals for a study of the genetic diversity of this species. A total of 27 ISSR primers were tested for their ability to amplify genomic DNA of three individuals (bulk); all of them amplified regions between the simple sequence repeats of the genome, however seven ISSR primers showed a good standard of amplification compared to the others. The seven primers selected were used to amplify the genomic DNA of nine individuals and generated a total of 78 loci, of which 52.7% were polymorphic. The content of polymorphic information of the primers ranged from 0.261 to 0.489, showing that selected primers are moderately informative; bootstrap analysis determined that 51 loci were enough to estimate the genetic diversity of the samples of individuals. We concluded that the markers generated by the primers UBC 807, UBC 824, UBC 827, UBC 840, UBC 851, UBC 873 and UBC 881 allow detection of the genetic polymorphism among individuals of M. caesalpiniaefolia, being useful to determine the genetic diversity of this species.
author	dos Santos Araújo,Fernando Vasconcelos Pacheco,Mauro de Almeida Vieira,Fábio dos Santos Ferrari,Cibele Cardoso Félix,Francival Teixeira das Chagas,Kyvia Pontes
author_facet	dos Santos Araújo,Fernando Vasconcelos Pacheco,Mauro de Almeida Vieira,Fábio dos Santos Ferrari,Cibele Cardoso Félix,Francival Teixeira das Chagas,Kyvia Pontes
author_sort	dos Santos Araújo,Fernando
title	ISSR molecular markers for the study of the genetic diversity of Mimosa caesalpiniaefolia Benth.
title_short	ISSR molecular markers for the study of the genetic diversity of Mimosa caesalpiniaefolia Benth.
title_full	ISSR molecular markers for the study of the genetic diversity of Mimosa caesalpiniaefolia Benth.
title_fullStr	ISSR molecular markers for the study of the genetic diversity of Mimosa caesalpiniaefolia Benth.
title_full_unstemmed	ISSR molecular markers for the study of the genetic diversity of Mimosa caesalpiniaefolia Benth.
title_sort	issr molecular markers for the study of the genetic diversity of mimosa caesalpiniaefolia benth.
publisher	Universidad de Tarapacá. Facultad de Ciencias Agronómicas
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-34292016000300007
work_keys_str_mv	AT dossantosaraujofernando issrmolecularmarkersforthestudyofthegeneticdiversityofmimosacaesalpiniaefoliabenth AT vasconcelospachecomauro issrmolecularmarkersforthestudyofthegeneticdiversityofmimosacaesalpiniaefoliabenth AT dealmeidavieirafabio issrmolecularmarkersforthestudyofthegeneticdiversityofmimosacaesalpiniaefoliabenth AT dossantosferraricibele issrmolecularmarkersforthestudyofthegeneticdiversityofmimosacaesalpiniaefoliabenth AT cardosofelixfrancival issrmolecularmarkersforthestudyofthegeneticdiversityofmimosacaesalpiniaefoliabenth AT teixeiradaschagaskyviapontes issrmolecularmarkersforthestudyofthegeneticdiversityofmimosacaesalpiniaefoliabenth
_version_	1714203719733608448

ISSR molecular markers for the study of the genetic diversity of Mimosa caesalpiniaefolia Benth.

Ejemplares similares